Molecular Neurobiology

, Volume 56, Issue 10, pp 6770–6776 | Cite as

Brain Metabolic DNA Is Reverse Transcribed in Cytoplasm: Evidence by Immunofluorescence Analysis

  • Marina Prisco
  • Joyce Casalino
  • Carolina Cefaliello
  • Antonio GiudittaEmail author


In a previous study (Mol Neurobiol 55:7476–7486, 2017), newly synthesized brain metabolic DNA (BMD) from rat subcellular fractions has been shown to behave as a DNA-RNA hybrid when analyzed in cesium gradients at early [3H] thymidine incorporation times but to assume the double-stranded configuration at later times. Conversely, BMD from purified nuclei displayed the dsDNA configuration even at early incorporation times. The results were interpreted to support the BMD origin by reverse transcription in the cytoplasm and its later acquisition of the double-stranded configuration before the partial transfer to the nuclei. This interpretation has now been confirmed by immunofluorescence analyses of newly synthesized BrdU-labeled BMD from the mouse brain that demonstrates its cytoplasmic localization and colocalization with DNA-RNA hybrids. In addition, BrdU-labeled BMD has been shown to colocalize with astroglial anti-GFAP antibodies and with presynaptic anti-synaptophysin antibodies.


Brain metabolic DNA (BMD) DNA synthesis Reverse transcription Astroglia Synaptosomes 



We warmly thank Prof. M. D’Amico and. S. Maione for their generous permission to use the facilities of the Second University of Naples Medical School.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Biology DepartmentUniversity of Naples Federico IINaplesItaly
  2. 2.Department of NeurologyUniversity of Massachusetts Medical SchoolWorcesterUSA
  3. 3.Accademia di Scienze Fisiche e MatematicheNaplesItaly

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