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Synthesis, characterization and anticancer activity of selenium nanobiocomposite of l-asparaginase

  • G BaskarEmail author
  • K Lalitha
  • Garrick Bikku George
Article

Abstract

Selenium is a rare earth nonmetal and an essential micronutrient for animals, and a trace nutrient for humans. It is known to function as a cofactor for reduction of antioxidant enzymes, such as glutathione peroxidase. The selenium nanobiocomposite synthesized using a co-precipitation method with sizes 20–30 nm were confirmed using scanning electron microscopy and they showed maximum absorption between 300 and 400 nm in the ultra-violet spectrum. Fourier transform infrared and H-nuclear magnetic resonance analysis revealed the involvement of various functional groups in the binding of asparaginase to selenium nanoparticles to form the selenium nanobiocomposite. X-ray diffraction analysis confirmed the hexagonal structure of the selenium nanobiocomposite. Methylthiazolyl diphenyl-tetrazolium bromide assay on HT-29, MG-63 and HEPTO cells loaded with the selenium nanobiocomposite revealed a toxicity of 93.62, 94.24 and 87.68%, respectively, for a selenium nanobiocomposite concentration of \(1000\,\upmu \hbox {g ml}^{-1}\). The selenium nanobiocomposite of l-asparaginase opens a new arena in cancer treatment.

Keywords

Selenium nanobiocomposite asparaginase anticancer activity colon cancer human osteosarcoma liver cancer 

Notes

Acknowledgements

This work was financially supported under the scheme Pilot Project Grant for Young Investigators in Cancer Biology (Sanction No. 6242-P96/RGCB/PMD/DBT/GBKR/2015) by the Department of Biotechnology, Government of India.

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of BiotechnologySt. Joseph’s College of EngineeringChennaiIndia

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