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Members of B-box Protein Family from Malus domestica Enhanced Abiotic Stresses Tolerance in Escherichia coli

  • Xin Liu
  • Yaqing Dai
  • Rong Li
  • Li Yuan
  • Xuesen Chen
  • Xiaoyun WangEmail author
Original paper
  • 37 Downloads

Abstract

The B-box proteins (BBXs) are zinc finger proteins containing one or two B-box domain(s) and involved in regulation of development processes as transcription factors in plants. Here, seven BBX genes in Malus domestica genome (MdBBXs) were identified and found to be up-regulated under abiotic stresses, with 2–12 folds in roots. All recombinant MdBBXs expressed in Escherichia coli (E. coli) enhanced the cell’s tolerance to salt and osmotic stresses, respectively. Deficiency of B-box domain of MdBBX10 led to the loss of anti-stress functions. Five conservative cysteines in B-box domain played crucial roles in stress resistance, which are involved in two of metal iron binding sites of zinc finger motifs in BBXs. All the above results suggested MdBBXs confer stress tolerance to E. coli cell against abiotic stresses.

Keywords

B-box domain Stress tolerance Zinc finger motifs Conservative cysteine 

Notes

Acknowledgements

This work was supported by Special Research Fund of Public Welfare of China Agricultural Ministry (201303093).

Author Contributions

Designed the experiments: XYW and XL. Performed the experiments: XL, YQD, RL, and LY. Analyzed the data: XL, YQD, and XSC. Wrote the paper: XYW and XL. All authors read and approved the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interests.

Supplementary material

12033_2019_172_MOESM1_ESM.docx (1.8 mb)
Supplementary material 1 (DOCX 1795 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Xin Liu
    • 1
  • Yaqing Dai
    • 1
  • Rong Li
    • 1
  • Li Yuan
    • 1
  • Xuesen Chen
    • 1
  • Xiaoyun Wang
    • 1
    Email author
  1. 1.College of Life Science, State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anPeople’s Republic of China

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