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Phage Display Libraries: From Binders to Targeted Drug Delivery and Human Therapeutics

  • Mouldy SioudEmail author
Review
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Abstract

The application of repertoire selection technologies for the study and characterization of tumor heterogeneity is an area of great interest in the field of tumor biology and immunotherapy. Among the most promising approaches, phage display has been successfully used to select peptides and antibody fragments to a variety of different targets, including cancer cells, immune cells and cytokines. Peptides selected from phage display have been used to guide the delivery of lytic peptides, cytotoxic drugs, and nanoparticles to cancer cells with the aim to obtain more efficient and less toxic therapeutics. Additionally, antibodies developed through phage display are being used in the treatment of autoimmune diseases and in some cases metastatic cancers. This review provides a short description of how phage libraries are designed, and highlights the conversion of the isolated binders into human therapeutics and use in targeted therapies.

Keywords

Phage display Affinity selection Peptides Antibodies Targeted therapies Drug delivery Immunotherapy Biomarkers 

Abbreviations

cDNA

Complementary DNA

CDR

Complementarity-determining region

EGF

Epidermal growth factor

Fab

Antigen-binding fragment

FDA

Food and drug administration

Ig

Immunoglobulin

mAb

monoclonal antibody

NSCLC

Non-small cell lung cancer

scFv

Single-chain variable fragment

TNF

Tumor necrosis factor

VEGF

Vascular endothelial growth factor

VH

Variable heavy chain

VL

Variable light chain

Notes

Acknowledgements

This work was supported by the Norwegian Cancer Society (Grant No. 182593).

Compliance with Ethical Standards

Conflict of interest

The author has no potential conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Cancer ImmunologyOslo University Hospital, The Norwegian Radium Hospital, MontebelloOsloNorway

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