Molecular Biotechnology

, Volume 60, Issue 11, pp 783–790 | Cite as

Purification and Biochemical Characterization of Phytase Enzyme from Lactobacillus coryniformis (MH121153)

  • Yeliz DemirEmail author
  • Neslihan Dikbaş
  • Şükrü Beydemir
Original Paper


Phytase (myo-inositol hexaphosphate phosphohydrolase) belongs to phosphatases. It catalyzes the hydrolysis of phytate to less-phosphorylated inorganic phosphates and phytate. Phytase is used primarily for the feeding of simple hermit animals in order to increase the usability of amino acids, minerals, phosphorus and energy. In the present study, phytase isolation from the Lactobacillus coryniformis strain, isolated from Lor cheese sources, phytase purification and characterization were studied. The phytase was purified in simple three steps. The enzyme was obtained with 2.60% recovery and a specific activity of 202.25 (EU/mg protein). The molecular mass of the enzyme was determined to be 43.25 kDa with the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The optimum temperature and pH for the enzyme were found as 60 °C and 5.0 and respectively. To defined the substrate specificity of the phytase, the hydrolysis of several phosphorylated compounds by the purified enzyme was studied and sodium phytate showed high specificity. Furthermore, the effects of Ca2+, Ag+, Mg2+, Cu2+, Co2+, Pb2+, Zn2+ and Ni2+ metal ions on the enzyme were studied.


Phytase Enzyme purification Lactobacillus coryniformis Metal ions 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of SciencesAtaturk UniversityErzurumTurkey
  2. 2.Department of Agricultural Biotechnology, Faculty of AgricultureAtaturk UniversityErzurumTurkey
  3. 3.Department of Biochemistry, Faculty of PharmacyAnadolu UniversityEskişehirTurkey

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