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Molecular Biotechnology

, Volume 60, Issue 11, pp 833–842 | Cite as

A Novel Molecular Design for a Hybrid Phage-DNA Construct Against DKK1

  • Saeed Khalili
  • Mohamad Javad Rasaee
  • Taravat Bamdad
  • Maysam Mard-Soltani
  • Majid Asadi Ghalehni
  • Abolfazl Jahangiri
  • Mohammad Hassan Pouriayevali
  • Mohammad Reza Aghasadeghi
  • Fatemeh Malaei
Original Paper

Abstract

Nucleic acid immunization has recently exhibited a great promise for immunotherapy of various diseases. However, it is now clear that powerful strategies are imminently needed to improve their efficiency. In this regard, whole bacteriophage particles have been described as efficient DNA vaccine delivery vehicles, capable of circumventing the limitations of naked DNA immunization. Moreover, phage particles could be engineered to display specific peptides on their surfaces. Given these inherent characteristics of phages, we have designed a novel hybrid phage-DNA immunization vector using both M13 and pAAV plasmid elements. Following the construction and in vitro confirmation of the designed vectors, they were used for comparative mice immunization, carrying the same DNA sequence. The results indicated the efficacy of the designed hybrid phage particles, to elicit higher humoral immunity, in comparison to conventional DNA-immunization vectors (pCI). In light of these findings, it could be concluded that using adeno-associated virus (AAV) expression cassette along with displaying TAT peptide on the surface of the phage particle could be deemed as an appealing strategy to enhance the DNA-immunization and vaccination efficacy.

Keywords

DNA vaccination Phage-DNA vaccination Peptide display DKK1 TAT peptide 

Notes

Acknowledgements

The authors wish to thank Tarbiat Modares University for supporting the conduct of this research.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Research Involving Human Participants and/or Animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

12033_2018_115_MOESM1_ESM.docx (690 kb)
Supplementary material 1 (DOCX 690 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Saeed Khalili
    • 1
    • 2
  • Mohamad Javad Rasaee
    • 2
  • Taravat Bamdad
    • 3
  • Maysam Mard-Soltani
    • 4
  • Majid Asadi Ghalehni
    • 5
  • Abolfazl Jahangiri
    • 6
  • Mohammad Hassan Pouriayevali
    • 7
  • Mohammad Reza Aghasadeghi
    • 7
  • Fatemeh Malaei
    • 2
  1. 1.Department of Biology SciencesShahid Rajaee Teacher Training UniversityTehranIran
  2. 2.Department of Medical Biotechnology, Faculty of Medical Sciences, School of Medical SciencesTarbiat Modares UniversityTehranIran
  3. 3.Department of Virology, School of Medical SciencesTarbiat Modares UniversityTehranIran
  4. 4.Department of Clinical Biochemistry, Faculty of Medical SciencesDezful University of Medical SciencesDezfulIran
  5. 5.Department of Medical Biotechnology, School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  6. 6.Applied Microbiology Research Center, Systems Biology and Poisonings InstituteBaqiyatallah University of Medical SciencesTehranIran
  7. 7.Department of Hepatitis and AIDSPasteur Institute of IranTehranIran

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