A Graft Mimic Strategy for Verticillium Resistance in Tomato
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Grafting vegetables for disease resistance has increased greatly in popularity over the past 10 years. Verticillium wilt of tomato is commonly controlled through grafting of commercial varieties on resistant rootstocks expressing the Ve1 R-gene. To mimic the grafted plant, proteomic analyses in tomato were used to identify a suitable root-specific promoter (TMVi), which was used to express the Ve1-allele in susceptible Craigella (Cs) tomato plants. The results indicate that when infected with Verticillim dahliae, race 1, the transformed plants are comparable to resistant cultivars (Cr) or grafted plants.
KeywordsPlant grafting Proteome Tissue-specific expression Tomato Verticillium wilt Ve R-locus Ve1-gene
We thank Drs. X. Luo and K.V. Soman (UTMB Proteomics Center) for the peptide mass spectrometry and help with the proteomic analyses. This study was supported by NSERC Canada (R.N.N. and E.J.R.) and NIH, NHLBI (A.K.).
RNN and JR conceived the experiments and prepared the manuscript, MM conducted the experiments. RNN conducted the data analyses and AK was responsible for the peptide mass spectrometry.
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