Journal of Molecular Neuroscience

, Volume 69, Issue 1, pp 1–16 | Cite as

Antioxidant and Anti-Apoptotic Activity of Octadecaneuropeptide Against 6-OHDA Toxicity in Cultured Rat Astrocytes

  • Hadhemi Kaddour
  • Yosra Hamdi
  • Fatma Amri
  • Seyma Bahdoudi
  • Ibtissem Bouannee
  • Jérôme Leprince
  • Sami Zekri
  • Hubert Vaudry
  • Marie-Christine Tonon
  • David Vaudry
  • Mohamed Amri
  • Sana Mezghani
  • Olfa Masmoudi-KoukiEmail author


Oxidative stress, associated with various neurodegenerative diseases, promotes ROS generation, impairs cellular antioxidant defenses, and finally, triggers both neurons and astroglial cell death by apoptosis. Astrocytes specifically synthesize and release endozepines, a family of regulatory peptides, including the octadecaneuropeptide (ODN). We have previously reported that ODN acts as a potent neuroprotective agent that prevents 6-OHDA-induced apoptotic neuronal death. The purpose of the present study was to investigate the potential glioprotective effect of ODN on 6-OHDA-induced oxidative stress and cell death in cultured rat astrocytes. Incubation of astrocytes with graded concentrations of ODN (10−14 to 10−8 M) inhibited 6-OHDA-evoked cell death in a concentration- and time-dependent manner. In addition, ODN prevented the decrease of mitochondrial activity and caspase-3 activation induced by 6-OHDA. 6-OHDA-treated cells also exhibited enhanced levels of ROS associated with a generation of H2O2 and O2°-, and a reduction of both superoxide dismutase (SOD) and catalase (CAT) activities. Co-treatment of astrocytes with low concentrations of ODN dose-dependently blocked 6-OHDA-evoked production of ROS and inhibition of antioxidant enzyme activities. Concomitantly, ODN stimulated Mn-SOD, CAT, glutathione peroxidase-1, and sulfiredoxin-1 gene transcription and rescued 6-OHDA-associated reduced expression of endogenous antioxidant enzymes. Taken together, these data indicate that, in rat astrocytes, ODN exerts anti-apoptotic and anti-oxidative activities, and hence prevents 6-OHDA-induced oxidative assault and cell death. ODN is thus a potential candidate to delay neuronal damages in various pathological conditions involving oxidative neurodegeneration.


Astrocytes Octadecaneuropeptide Oxidative stress Cell death 6-hydroxydopamine Neuroprotection 





Central nervous system


Diazepam-binding inhibitor




5,5″,6,6″-tetrachloro-1,1″,3,3″-tetraethylbenzimidazolylcarbocyanine iodide




Dihydrorhodamine 123


Fluorescein diacetate


Glutathione peroxidase


Hydrogen peroxide


Lactate dehydrogenase


Nitrotetrazolium blue chloride


Superoxide anions




Parkinson’s disease


Reactive oxygen species


Superoxide dismutase





This work was supported by the France-Tunisia CMCU-Campus France/PHC Utique 16G0820/34940PK exchange program (to Olfa Masmoudi-Kouki and David Vaudry), Alternance scholarship of Tunisian Higher Education Ministry and the Laboratory of Neurophysiology, Cellular Physiopathology and Biomolecules Valorisation. LR18ES03, INSERM (U1239), the European Regional Development Fund (PACT-CBS and PHEDERCPG projects), and the Region Normandy. Europe gets involved in Normandy with European Regional Development Fund (ERDF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Hadhemi Kaddour
    • 1
    • 2
    • 3
  • Yosra Hamdi
    • 1
  • Fatma Amri
    • 1
  • Seyma Bahdoudi
    • 1
    • 4
  • Ibtissem Bouannee
    • 1
  • Jérôme Leprince
    • 4
    • 5
  • Sami Zekri
    • 6
  • Hubert Vaudry
    • 4
    • 5
  • Marie-Christine Tonon
    • 4
  • David Vaudry
    • 4
    • 5
  • Mohamed Amri
    • 1
  • Sana Mezghani
    • 1
  • Olfa Masmoudi-Kouki
    • 1
    Email author
  1. 1.University Tunis El Manar, Faculty of Sciences of Tunis, LR18ES03, Laboratory of NeurophysiologyCellular Physiopathology and Biomelcules ValorisationTunisTunisia
  2. 2.CIRB, CNRS UMR 7241/INSERM U1050PSL University, Labex MemoLife, Collège de FranceParisFrance
  3. 3.Imagine Institute and Center of Psychiatry and NeuroscienceUniversité Paris Descartes, 102-108 rue de la SantéParisFrance
  4. 4.UNIROUEN, Inserm U1239, Laboratory of Neuronal and Neuroendocrine Communication and DifferentiationNormandie UnivRouenFrance
  5. 5.UNIROUEN, Regional Cell Imaging Platform of Normandy (PRIMACEN)Normandie UnivRouenFrance
  6. 6.USCR Transmission Electron Microscopy, Faculty of MedicineUniversity Tunis El ManarTunisTunisia

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