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Journal of Molecular Neuroscience

, Volume 66, Issue 2, pp 291–305 | Cite as

Continuous Exposure to Inorganic Mercury Affects Neurobehavioral and Physiological Parameters in Mice

  • Hafsa Malqui
  • Hammou Anarghou
  • Fatima Zahra Ouardi
  • Nabila Ouasmi
  • Mohamed Najimi
  • Fatiha Chigr
Article
  • 56 Downloads

Abstract

Contamination with mercury is a real health issue for humans with physiological consequences. The main objective of the present study was to assess the neurotoxicological effect of inorganic mercury: HgCl2. For this, adult mice were exposed prenatally, postnatally, and during the adult period to a low level of the metal, and their behavior and antioxidant status were analyzed. First, we showed that mercury concentrations in brain tissue of treated animals showed significant bioaccumulation, which resulted in behavioral deficits in adult mice. Thus, the treated mice developed an anxiogenic state, as evidenced by open field and elevated plus maze tests. This anxiety-like behavior was accompanied by a decrease in social behavior. Furthermore, an impairment of memory in these treated mice was detected in the object recognition and Y-maze tests. The enzymatic activity of the antioxidant system was assessed in eight brain structures, including the cerebral cortex, olfactory bulb, hippocampus, hypothalamus, mesencephalon, pons, cerebellum, and medulla oblongata. The results show that chronic exposure to HgCl2 caused alterations in the activity of catalase, thioredoxin reductase, glutathione peroxidase, superoxide dismutase, and glutathione S-transferase, accompanied by peroxidation of membrane lipids, indicating a disturbance in intracellular redox homeostasis with subsequent increased intracellular oxidative stress. These changes in oxidative stress were concomitant with a redistribution of essential heavy metals, i.e., iron, copper, zinc, and magnesium, in the brain as a possible response to homeostatic dysfunction following chronic exposure. The alterations observed in overall oxidative stress could constitute the basis of the anxiety-like state and the neurocognitive disorders observed.

Keywords

Mercury Neurotoxicity Antioxidant system Neurobehavior Anxiety 

Notes

Author Contributions

HM, FC, and MN designed the experiments and performed the analysis of the data; HM, HA, NO, and FZO performed the experiments and assembled the figures. All the authors wrote or edited and validated the manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Authors and Affiliations

  1. 1.Biological Engineering Laboratory, Faculty of Sciences and TechniquesSultan Moulay Slimane UniversityBeni MellalMorocco

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