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Journal of Molecular Neuroscience

, Volume 66, Issue 2, pp 163–171 | Cite as

p38 Inhibitor Protects Mitochondrial Dysfunction by Induction of DJ-1 Mitochondrial Translocation After Subarachnoid Hemorrhage

  • Liyong Huang
  • Yaqing Hou
  • Lei Wang
  • Xiahui Xu
  • Qingkai Guan
  • Xiangsheng Li
  • Ying Chen
  • Wenke Zhou
Article
  • 93 Downloads

Abstract

p38 mitogen-activated protein kinase (MAPK) is a major player in mitochondrial dysfunction after subarachnoid hemorrhage (SAH). Moreover, DJ-1, which responds to oxidative stress and translocates to mitochondria, maintains mitochondrial homeostasis. Although a few studies have demonstrated that DJ-1 indirectly regulates p38 activation, the relationship between DJ-1 and p38 in mitochondrial dysfunction after SAH has not been delineated. Using an in vitro SAH model, alterations in p38, p-p38, DJ-1, and autophagic-related protein expression were detected. As expected, p38 inhibitor significantly blocked excessive expression of p38 and p-p38 after SAH, whereas total DJ-1 expression and mitochondrial DJ-1 were up-regulated. Further analysis showed that p38 inhibitor significantly blocked oxyhemoglobin (OxyHb) induced mitochondrial dysfunction, including mitochondrial membrane potential depolarization and reactive oxygen species (ROS) release. In addition, p38 inhibitor restored OxyHb-induced abnormal autophagic flux at the initiation and formation stage by regulating Atg5, beclin-1, the ratio of LC3-II/LC3-I, and p62 expression. This study suggested that overexpression of p38 induced the accumulation of mitochondrial dysfunction partly due to abnormal activation of autophagy, which largely relied on DJ-1 mitochondrial translocation.

Keywords

p38 DJ-1 Mitochondrial dysfunction ROS Autophagy 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81541030) and Henan Province of Natural Science Foundation (No. 182300410299).

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflicts of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeurosurgeryThe First Affiliated Hospital of Xinxiang Medical UniversityWeihuiChina
  2. 2.College of Life ScienceHenan Normal UniversityXinxiangChina

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