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Journal of Molecular Neuroscience

, Volume 66, Issue 2, pp 157–162 | Cite as

Genetic Variation of the Kalirin Gene is Associated with ICAS in the Chinese Population

  • Meizheng Dang
  • Yue Song
  • Qunying Li
  • Chao Zhang
  • Yanqing Peng
  • Zhenli Wei
  • Pintong Huang
Article

Abstract

Intracranial atherosclerotic stenosis (ICAS) is one of the most common causes of ischemic stroke (IS), especially in recurrent patients. Several studies have demonstrated an intimate association between the genetic mutation of the KALRN gene and IS. The main aim of this study was to clarify the relationship between three single nucleotide polymorphisms (SNPs) of KALRN and ICAS in a northern Chinese population and further explore the underlining mechanism. The relationship between each SNP and ICAS and the related components were examined in five models (co-dominant, dominant, recessive, heterozygous, and additive). Multivariate logistic regression models were applied and adjusted for 10,000 permutation tests. Haplotype regression analysis was performed to determine the associations between haplotypes and the severity of ICAS. qRT-PCR was used to confirm the mRNA level of Kalirin. SNP rs6438833 of the Kalirin gene in the additive model was associated with ICAS (OR = 1.642, 95%CI: 1.129-2.388, PDom = 0.048). After performing 10,000 permutations, the result showed a trend toward statistical significance (Pper = 0.048). Haplotypes of the KALRN gene were not significantly associated with ICAS (global significance, p = 0.090). qRT-PCR showed that the expression of KALRN 9 in IS patients was almost threefold the control group (p < 0.0005). SNPs of the KALRN gene are associated with ICAS in the northern Chinese population.

Keywords

Ischemic stroke Intracranial atherosclerotic stenosis KALRN SNP 

Notes

Acknowledgements

The study was supported by the National Natural Science Foundation of China (Grant No. 81527803, 81420108018). We would like to express our appreciation to the participants of the study for their participation and full cooperation.

Compliance with Ethical Standards

Conflicts of Interest

None of the authors had any conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Meizheng Dang
    • 1
  • Yue Song
    • 1
  • Qunying Li
    • 1
  • Chao Zhang
    • 1
  • Yanqing Peng
    • 2
  • Zhenli Wei
    • 3
  • Pintong Huang
    • 1
  1. 1.Department of UltrasoundThe 2nd Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina
  2. 2.Department of UltrasoundPeople’s Hospital of Zhejiang ProvinceHangzhouChina
  3. 3.Department of Clinical LaboratoryThe 2nd Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina

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