CpG Islands Methylation Analysis of CDH11, EphA5, and HS3ST2 Genes in Gastric Adenocarcinoma Patients

  • Shirin Eyvazi
  • Amir Mahdi Khamaneh
  • Vahideh Tarhriz
  • Mojgan Bandehpour
  • Mohammad Saeid Hejazi
  • Amir Taher Eftekhar Sadat
  • Bita SepehriEmail author
Original Research



Gastric cancer is an aggressive disease which is the fourth prevalent malignancy in the world. Beside the genetic factors, epigenetic alterations such as promoter CpG island hyper methylation are involved in the emergence of gastric cancer. Herein, we investigated the methylation status of CDH11, EphA5, and HS3ST2 genes in patients with and without gastric adenocarcinoma for the first time.


In the study 40 paraffin-embedded tissue sections from gastric adenocarcinoma patients and 40 specimens from patients with functional dyspepsia were taken. DNA extraction was performed using a modified salting out method. Epizen DNA methylation kit was used to the bisulfite DNA conversion. The methylation status of CDH11, EphA5, and HS3ST2 genes were analyzed by methylation-specific PCR (MSP) technique.


Among the 80 specimens, 71 DNA samples were achieved (34 gastric adenocarcinoma patients and 37 control patients). The results showed that CDH11, EphA5, and HS3ST2 genes are methylated in 28 (82.45%), 19 (55.88%), and 26 (76.47%) of 34 DNA samples from gastric adenocarcinoma patients, respectively, whereas, these genes are methylated in 7 (18.91%), 9 (24.32%) and 7 (18.91%) of 37 samples from noncancerous patients, respectively. Statistical analyses using a chi-squared test showed that there is a statistically significant difference in methylation level of CDH11, EphA5, and HS3ST2 genes between gastric cancer and uncancerous patients (p < 0.05).


To the best of our knowledge, this is the first report on methylation of CDH11, EphA5, and HS3ST2 promoters’ in gastric adenocarcinoma patients using MSP. Identification of novel cancer-related molecular mechanisms can be useful in detection of new treatment strategies.


Gastric adenocarcinoma Hyper methylation MSP CDH11 EphA5 HS3ST2 



This work was funded and supported by Liver and Gastrointestinal Diseases Research Center of Tabriz University of Medical Sciences (Grantnumber: 61877) and Molecular Medicine Research Center, Bio‐medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.

Compliance with Ethical Standards

The present study was approved by the Ethics and Research Committees of the Tabriz University of Medical Sciences.

Conflict of Interest

The authors report no conflicts of interest in this work.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shirin Eyvazi
    • 1
    • 2
  • Amir Mahdi Khamaneh
    • 3
  • Vahideh Tarhriz
    • 4
  • Mojgan Bandehpour
    • 2
  • Mohammad Saeid Hejazi
    • 4
  • Amir Taher Eftekhar Sadat
    • 5
  • Bita Sepehri
    • 6
    Email author
  1. 1.Biotechnology Research CenterTabriz University of Medical SciencesTabrizIran
  2. 2.Department of Biotechnology, School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Department of Molecular Medicine, Faculty of Advanced Medical SciencesTabriz University of Medical SciencesTabrizIran
  4. 4.Molecular Medicine Research CenterTabriz University of Medical SciencesTabrizIran
  5. 5.Pathology DepartmentImam Reza Hospital, Tabriz University of Medical SciencesTabrizIran
  6. 6.Liver and Gastrointestinal Disease Research CenterTabriz University of Medical SciencesTabrizIran

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