Assessment of MMP29 Gene Expression and Silver Nanoparticles Effects on Colon Cancer Cell Line (HT29)
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Colon cancer is a major cause of death around the world. The evaluation of novel approaches on corresponding genes would be a vital strategy toward eradication of cancer cells. In this study, the toxicity of silver nanoparticle on the colon cancer cell line (HT29) and expression of matrix metalloproteinase (MMP29) gene was investigated.
Materials and Methods
The silver nanoparticle (AgNPs) was synthesized and assessed by transmission electron microscopy (TEM). The cytotoxicity of synthesized AgNP on the HT29 cell line was evaluated using the MTT assay. Furthermore, the expression of MMP29 gene was investigated by the quantitative real-time PCR (RT-qPCR).
The TEM results revealed that the fabricated AgNPs were mostly spherical in shape and had an average diameter of 22 nm. The results outlined that AgNPs significantly decreased the viability of cells in a dose- and time-dependent manner (p < 0.001). Additionally, we observed a significant difference among various concentrations.
The findings indicated that the green fabricated AgNPs have the potential as a promising approach toward the colon cancer therapy. Furthered studies are essential to evaluate against other cancer cell lines and genes participating in the cancer progress.
KeywordsAgNPs HT29 Matrix metalloproteinase 9 Colon Cancer Cytotoxicity
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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