Disregulation of miR-216a and miR-217 in Gastric Cancer and Their Clinical Significance
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The majority of gastric cancer (GC) diagnoses occur at the middle or late stage of the disease, indicating that finding novel biomarkers that could be detectable at earlier stage is urgently needed. Accumulating studies have shown that microRNAs, a class of tiny single-stranded RNAs, play important roles in multiple biological processes including cancer development. The present study aimed to evaluate the effect of miR-216a and miR-217 in GC.
Material and Methods
The real-time quantitative reverse-transcription PCR was exploited to identify and compare the expression levels of miR-216a and miR-217 in 37 pairs of samples of gastric cancer tissue and adjacent normal tissue. Superimposed on this, the potential relationship between miR-216a/217 levels and clinicopathological parameters in patients suffering GC was explored.
The results obtained from this study showed that the miR-216a is significantly upregulated in gastric cancer tissues, compared with adjacent normal tissues, but the altered expression of miR-217 was not significant. For miR-216a/217, no significant correlations were detected between expression levels of these miRNAs and clinical and pathological characteristics of patients.
This prospective study proposes that upregulation of miR-216a might represent an important mechanism for the development of gastric cancer.
KeywordsmiR-216a miR-217 Gastric cancer Expression analysis qPCR
We express our gratitude to the patients and the staff within the Endoscopy Department of Tabriz Emam Reza and Sina Hospitals and the faculty and staff at Department of Biology of the Tabriz University for their helpful collaborations.
This work was supported by grants from the Department of Biology, Faculty of Natural Sciences, Tabriz University.
Compliance with Ethical Standards
Conflict of Interest
The authors declare no conflict of interest.
- 2.Malekzadeh R, Derakhshan MH, Malekzadeh Z. Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med. 2009;12(6):576–83.Google Scholar
- 10.Felekkis K, Touvana E, Stefanou C, Deltas C. microRNAs: a newly described class of encoded molecules that play a role in health and disease. Hippokratia. 2010;14(4):236–40.Google Scholar
- 11.Wong N, Wang X. miRDB: an online resource for microRNA target prediction and functional annotations. Nucleic Acids Res. 2014:doi. https://doi.org/10.1093/nar/gku1104.
- 15.Almhanna K, Strosberg J, Malafa M. Targeting AKT protein kinase in gastric cancer. Anticancer Res. 2011;31(12):4387–92.Google Scholar
- 16.Dicken BJ, Bigam DL, Cass C, Mackey JR, Joy AA, Hamilton SM. Gastric adenocarcinoma: review and considerations for future directions. Ann Surg. 2005;241(1):27–39.Google Scholar
- 20.Volkomorov V, Grigoryeva E, Krasnov G, Litviakov N, Tsyganov M, Karbyshev M, et al. Search for potential gastric cancer markers using miRNA databases and gene expression analysis. Exp Oncol. 2013;35(1):2–7.Google Scholar
- 26.Inui M, Martello G, Piccolo S. MicroRNA control of signal transduction. Nat Rev Mol Cell Biol. 2010;11(4):252–63. http://www.nature.com/nrm/journal/v11/n4/suppinfo/nrm2868_S1.html.
- 28.Piguet A-C, Dufour J-F. PI(3)K/PTEN/AKT pathway. Journal of Hepatology. 54(6):1317–9. https://doi.org/10.1016/j.jhep.2010.12.013.