Immunologic Research

, Volume 66, Issue 6, pp 726–736 | Cite as

Routinely utilized in-house assays for infliximab, adalimumab and their anti-drug antibody levels

  • Manca Ogrič
  • Polona Žigon
  • David Drobne
  • Borut Štabuc
  • Snezna Sodin-Semrl
  • Saša ČučnikEmail author
  • Sonja Praprotnik
Original Article


By monitoring serum concentrations of infliximab (IFX) and adalimumab (ADL) and levels of their antibodies in patients with inflammatory diseases, clinicians can adjust dose and increase safety and effectiveness of treatment. The aim was to develop and validate in-house enzyme-linked immunosorbent assays (ELISAs) for IFX and ADL, together with anti-IFX and anti-ADL ELISAs for routine detection and further analysis with acid dissociation of immune complexes. Furthermore, the objectives were to compare in-house assays with commercial ELISAs and reporter gene assays (RGAs) and to determine cross-reactivity between original Remicade®/Remsima™ and their antibodies. In-house ELISAs were validated (imprecision, accuracy among other criteria) and compared with commercial apDia ELISAs and RGAs. Correlation coefficients, intraclass correlation coefficients, agreement, and bias were calculated. All in-house ELISAs gave precise and repeatable results. The immune complexes between IFX and anti-IFX were found in 3% of samples, while complexes between ADL and anti-ADL were found in 14% of samples. Significant correlations were found between in-house and apDia ELISAs and RGA for IFX, ADL, anti-IFX, and anti-ADL results. Remicade®, Remsima™, and their antibodies could be accurately measured with either apDia or in-house IFX and anti-IFX ELISAs. Accurate and precise in-house ELISAs, highly comparable with commercial ELISAs and RGAs, were developed and validated for routine analysis of samples of patients treated with IFX (Remicade® or Remsima™) or ADL providing substantial cost benefit. Complex dissociation identified samples with anti-IFX-IFX (3%) and anti-ADL-ADL (14%) complexes indicating the benefit of adding acid dissociation to therapeutic drug monitoring of IFX and ADL.


Infliximab Adalimumab Anti-drug antibodies ELISA Reporter gene assay Biosimilars 



The authors would like to thank all the staff in the Department of Rheumatology and the Immunology Laboratory, who helped collect and process the patient samples.

Research funding

The authors received financial support from the Slovenian Research Agency for funding the National Research Program “Systemic Autoimmune Diseases” (No. P3-0314).

Author contributions

All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Compliance with ethical standards

 This study was conducted as part of the National Research Program (No. P3-0314) and has been approved by the Slovenian Ethical Committee (#99/04/15 and #38/01/16). It has therefore been performed in accordance with the ethical standards laid down in 1964 Declaration of Helsinki and its later amendments. Informed consent was obtained from all individuals included in this study.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Manca Ogrič
    • 1
    • 2
  • Polona Žigon
    • 1
  • David Drobne
    • 3
    • 4
  • Borut Štabuc
    • 3
    • 4
  • Snezna Sodin-Semrl
    • 1
    • 5
  • Saša Čučnik
    • 1
    • 2
    Email author
  • Sonja Praprotnik
    • 1
  1. 1.Department of RheumatologyUniversity Medical Centre LjubljanaLjubljanaSlovenia
  2. 2.Faculty of Pharmacy, Chair of Clinical BiochemistryUniversity of LjubljanaLjubljanaSlovenia
  3. 3.Department of GastroenterologyUniversity Medical Centre LjubljanaLjubljanaSlovenia
  4. 4.Faculty of MedicineUniversity of LjubljanaLjubljanaSlovenia
  5. 5.Faculty of Mathematics, Natural Sciences and Information TechnologiesUniversity of PrimorskaKoperSlovenia

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