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Endocrine

pp 1–10 | Cite as

Early weaning leads to specific glucocorticoid signalling in fat depots of adult rats

  • Rosiane Aparecida Miranda
  • Carla Bruna Pietrobon
  • Iala Milene Bertasso
  • Vanessa S. Tavares Rodrigues
  • Bruna Pereira Lopes
  • Camila Calvino
  • Elaine de Oliveira
  • Egberto Gaspar de Moura
  • Patrícia C. LisboaEmail author
Original Article

Abstract

Purpose

Early weaning (EW) is a stressful condition that programmes a child to be overweight in adult life. Fat mass depends on glucocorticoids (GC) to regulate adipogenesis and lipogenesis. We hypothesised that the increased adiposity in models of EW was due to a disturbed HPA axis and/or disrupted GC function.

Methods

We used two experimental models, pharmacological early weaning (PEW, dams were bromocriptine-treated) and non-pharmacological early weaning (NPEW, dams’ teats were wrapped with a bandage), which were initiated during the last 3 days of lactation. Offspring from both genders was analysed on postnatal day 180.

Results

Offspring in both models were overweight with increased visceral fat mass, but plasma corticosterone was increased in both genders in the PEW group but not the NPEW group. NPEW males had increased GRα expression in visceral adipose tissue (VAT), and GRα expression decreased in PEW males in subcutaneous adipose tissue (SAT). Females in both EW groups had increased 11βHSD1 expression in SAT. PEW males had increased C/EBPβ expression in SAT. PEW females had lower PPARy and FAS expression in VAT than the NPEW females. We detected a sex dimorphism in VAT and SAT in the EW groups regarding 11βHSD1, GRα and C/EBPβ expression.

Conclusions

The accumulated adiposity induced by EW exhibited distinct mechanisms depending on gender, specific fat deposition and GC metabolism and action. The higher proportion of VAT/SAT in both sets of EW males may be related to the action of GC in these tissues, and the higher conversion of GC in SAT in females may explain the differences in the fat distribution.

Keywords

Early weaning Glucocorticoids Adipogenesis and lipogenesis 

Notes

Acknowledgements

All authors are grateful to Mr Ulisses Risso Siqueira for animal care and to Mrs Fabiana Gallaulckydio for technical assistance.

Funding

This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Estado do Rio de Janeiro (FAPERJ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The Institutional Ethical Committee for the use of laboratory animals of the Biology Institute, State University of Rio de Janeiro approved all experimental procedures (CEUA/035/2017). This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rosiane Aparecida Miranda
    • 1
  • Carla Bruna Pietrobon
    • 1
  • Iala Milene Bertasso
    • 1
  • Vanessa S. Tavares Rodrigues
    • 1
  • Bruna Pereira Lopes
    • 1
  • Camila Calvino
    • 1
  • Elaine de Oliveira
    • 1
  • Egberto Gaspar de Moura
    • 1
  • Patrícia C. Lisboa
    • 1
    Email author
  1. 1.Laboratory of Endocrine Physiology, Biology InstituteRio de Janeiro State UniversityRio de JaneiroBrazil

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