Maternal cinnamon intake during lactation led to visceral obesity and hepatic metabolic dysfunction in the adult male offspring
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Studies with foods, known to promote health benefits in addition to the nutritive value, show that their consumption by pregnant and/or lactating females could induce negative outcomes to the offspring. It is well characterized that cinnamon intake promotes benefits to energy homeostasis. The present study aimed to analyze the effects of the consumption of an aqueous extract of cinnamon by lactating female rats on the endocrine-metabolic outcomes in the adult offspring.
Lactating dams (Wistar rats) were supplemented with cinnamon aqueous extract (400 mg/kg body weight/day) for the entire lactating period. The male adult offspring were evaluated at 180 days old (CinLac).
The offspring presented visceral obesity (P = 0.001), hyperleptinemia (P = 0.002), and hyperinsulinemia (P = 0.016). In the liver, CinLac exhibited reduced p-IRβ (P = 0.018) suggesting insulin resistance. However, phosphorylation of IRS1 (P = 0.041) and AKT (P = 0.050) were increased. JAK2 (P = 0.030) and p-STAT3 (P = 0.015) expressions were higher, suggesting that the activation of IRS1/AKT in the CinLac group could have resulted from the increased activation of leptin signaling. Although we observed no changes in the gluconeogenic pathway, the CinLac group exhibited lower hepatic glycogen content (P = 0.005) accompanied by increased p-GSK3β (P = 0.011). In addition, the CinLac group showed increased hepatic triacylglycerol content (P = 0.049) and a mild steatosis (P = 0.001), accompanied by reduced PPARα mRNA expression (P = 0.005).
We conclude that maternal intake of aqueous extract of cinnamon induces long-term molecular, metabolic, and hormonal changes in the adult progeny, including visceral obesity, higher lipid accumulation, and lower glycogen content in the liver.
KeywordsLactation Metabolic programming Cinnamon Liver Insulin Leptin
This study was funded by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ; K.J.O., grant number 102.982) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). J.G.O.N. was recipient of a FAPERJ fellowship. T.B.B. was recipient of a CAPES fellowship.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal use and experimental procedures were approved by Ethics Committee on Animal Use of Fluminense Federal University under number 0120/11, and complied with the ethical guidelines of the Brazilian Society of Laboratory Animal Science.
- 2.B. Hanley, J. Dijane, M. Fewtrell, A. Grynberg, S. Hummel, C. Junien, B. Koletzko, S. Lewis, H. Renz, M. Symonds, M. Gros, L. Harthoorn, K. Mace, F. Samuels, E.M. van Der, Beek, Metabolic imprinting, programming and epigenetics—a review of present priorities and future opportunities. Br. J. Nutr. 104, S1 (2010)CrossRefPubMedGoogle Scholar
- 11.M.S. Figueiredo, M.C. da Fonseca Passos, I.H. Trevenzoli, A.A. Troina, A.S. Carlos, C.C. Alves Nascimento-Saba, M.C. Fraga, A.C. Manhães, E. de Oliveira, P.C. Lisboa, E.G. de Moura, Adipocyte morphology and leptin signaling in rat offspring from mothers supplemented with flaxseed during lactation. Nutrition 28, 307 (2012)CrossRefPubMedGoogle Scholar
- 13.P. B. Wilson, A population-representative analysis of dietary supplementation among Americans with diabetes mellitus. J. Diabetes (2018). https://doi.org/10.1111/jdb.12815
- 14.T. Sartorius, A. Peter, N. Schulz, A. Drescher, I. Bergheim, J. Machann, F. Schick, D. Siegel-Axel, A. Schürmann, C. Weigert, H.-U. Häring, A.M. Hennige, Cinnamon extract improves insulin sensitivity in the brain and lowers liver fat in mouse models of obesity. PLoS One 9, e92358 (2014)CrossRefPubMedPubMedCentralGoogle Scholar
- 19.B.P. Lopes, T.G. Gaique, L.L. Souza, G.S.M. Paula, G.E.G. Kluck, G.C. Atella, A.C.C. Gomes, N.K. Simas, R.M. Kuster, T.M. Ortiga-Carvalho, C.C. Pazos-Moura, K.J. Oliveira, Cinnamon extract improves the body composition and attenuates lipogenic processes in the liver and adipose tissue of rats. Food Funct. 6, 3257 (2015)CrossRefPubMedGoogle Scholar
- 20.P. Ranasinghe, R. Jayawardana, P. Galappaththy, G.R. Constantine, N. de Vas Gunawardana, P. Katulanda, Efficacy and safety of ‘true’ cinnamon ( Cinnamomum zeylanicum ) as a pharmaceutical agent in diabetes: a systematic review and meta-analysis: efficacy and safety of ‘true’ cinnamon in diabetes. Diabet. Med. 29, 1480 (2012)CrossRefPubMedGoogle Scholar
- 29.A. Mann, A. Thompson, N. Robbins, A.L. Blomkalns, Localization, identification, and excision of murine adipose depots. J. Vis. Exp 94, e52174 (2014)Google Scholar
- 30.T.G. Gaique, B.P. Lopes, L.L. Souza, G.S.M. Paula, C.C. Pazos-Moura, K.J. Oliveira, Cinnamon intake reduces serum T3 level and modulates tissue-specific expression of thyroid hormone receptor and target genes in rats: Cinnamon and thyroid function. J. Sci. Food Agric. 96, 2889 (2016)CrossRefPubMedGoogle Scholar
- 33.E.D.C. Frantz, R.F. Medeiros, I.G. Giori, J.B.S. Lima, T. Bento-Bernardes, T.G. Gaique, C. Fernandes-Santos, T. Fernandes, E.M. Oliveira, C.P. Vieira, C.A. Conte-Junior, K.J. Oliveira, A.C.L. Nobrega, Exercise training modulates the hepatic renin-angiotensin system in fructose-fed rats: exercise training modulates hepatic renin-angiotensin system. Exp. Physiol. 102, 1208 (2017)CrossRefPubMedGoogle Scholar
- 35.S. Sam, Differential effect of subcutaneous abdominal and visceral adipose tissue on cardiometabolic risk. Horm. Mol. Biol. Clin. Investig. 33, (2018). https://doi.org/10.1515/hmbci-2018-0014
- 37.S. Fruhwürth, H. Vogel, A. Schürmann, K.J. Williams, Novel insights into how overnutrition disrupts the hypothalamic actions of leptin. Front. Endocrinol. 9, (2018). https://doi.org/10.3389/fendo.2018.00089
- 56.P.-T. Xu, Z. Song, W.-C. Zhang, B. Jiao, Z.-B. Yu, Impaired translocation of GLUT4 results in insulin resistance of atrophic soleus muscle. Biomed. Res. Int. 2015, 1 (2015)Google Scholar
- 61.C.P. Dias-Rocha, M.M. Almeida, E.M. Santana, J.C.B. Costa, J.G. Franco, C.C. Pazos-Moura, I.H. Trevenzoli, Maternal high-fat diet induces sex-specific endocannabinoid system changes in newborn rats and programs adiposity, energy expenditure and food preference in adulthood. J. Nutr. Biochem. 51, 56–68 (2018)CrossRefPubMedGoogle Scholar
- 62.V.S.T. Rodrigues, E.G. Moura, D.N. Bernardino, J.C. Carvalho, P.N. Soares, T.C. Peixoto, N. Peixoto-Silva, E. Oliveira, P.C. Lisboa, Supplementation of suckling rats with cow’s milk induces hyperphagia and higher visceral adiposity in females at adulthood, but not in males. J. Nutr. Biochem. 55, 89–103 (2018)CrossRefPubMedGoogle Scholar