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Stem Cell Reviews and Reports

, Volume 15, Issue 5, pp 717–729 | Cite as

A Retrospective Analysis of Safety and Efficacy of Wharton’s Jelly Stem Cell Administration in Children with Spina Bifida

  • Dariusz BoruczkowskiEmail author
  • Izabela Zdolińska-Malinowska
Original Article

Abstract

The aim of this paper was to describe the outcome of therapeutic administration of mesenchymal stem cells (MSC) obtained from Wharton’s jelly (WJ-MSCs) in paediatric patients with spina bifida (SB) during a medical therapeutic experiment. We retrospectively analysed the records of twenty-eight patients aged 1–18 years (median age 4 years) recruited in daily clinical practice. Each patient received 0.9–5.0 × 106 WJ-MSCs/kg (median 2.6 × 106 WJ-MSCs/kg) administered in 1–5 injections as an experimental treatment for SB (allogenic administration). All the patients were examined by the same neurologist (study investigator, SI) on the day of each infusion. Based on the neurological examination, the SI used a six-point Likert scale to assess the quality of life and self-service of each patient. Twenty-six follow-up observations after MSC administration were analysed retrospectively. In addition, the assessments of the parents and other healthcare professionals were obtained for 5 patients and compared with the SI’s assessment. Twenty-one of 26 patients (81%) experienced some improvement in their health status. Twenty-one (81%) patients experienced increased quality of life (median 2.0) and 10 patients (38%) achieved a slight increase in their self-service level (median 1). Improvement was achieved in 12 out of 17 areas. Five were significant in low-power sign test: muscle tension, muscle strength, gross motor development, micturition/defecation control, and cognitive functions. Adverse events were mild and temporary. Age, body mass, single dose or poor response after the first administration were not significant predictors of later response to treatment in contrast to the total cell dose per one kg in the whole treatment course. WJ-MSC administration is a safe and effective procedure that improves motor functions, micturition/defecation control, and cognitive functions, and improves the quality of life in children with SB.

Keywords

Spina bifida Myelomeningocele Stem cell Therapy Treatment MSC Mesenchymal stem cells Congenital spinal malformations 

Notes

Acknowledgements

The authors would like to thank Magdalena Chrościńska-Krawczyk from the Department of Paediatric Neurology, Lublin Medical University, Lublin, Poland, for the qualification of patients, supervision during the therapy and medical examination during follow-ups. They also wish to thank Tomasz Ołdak from the Laboratory at Polski Bank Komórek Macierzystych S.A. (FamiCord Group), Warsaw, Poland, for his supervision during the preparation of Wharton’s jelly-derived mesenchymal stem cells. The collection of biological material was carried out as part of the commercial services provided by Polski Bank Komórek Macierzystych S.A., with the participation of medical staff from public hospitals and employees of Polski Bank Komórek Macierzystych S.A. Language assistance was provided by Marisa Granados.

Funding

The study was sponsored by Polski Bank Komórek Macierzystych S.A. (FamiCord Group), Warsaw, Poland.

Compliance with Ethical Standards

Conflict of Interest

Both authors are employees of Polski Bank Komórek Macierzystych S.A. (FamiCord Group), Warsaw, Poland.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Medical DepartmentPolski Bank Komórek Macierzystych S.A. (FamiCord Group)WarsawPoland

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