Neonatal overfeeding impairs differentiation potential of mice subcutaneous adipose mesenchymal stem cells

  • Isabelle Dias
  • Ísis Salviano
  • André Mencalha
  • Simone Nunes de Carvalho
  • Alessandra Alves Thole
  • Laís Carvalho
  • Erika Cortez
  • Ana Carolina Stumbo
Article
  • 1 Downloads

Abstract

Nutritional changes in the development (intrauterine life and postnatal period) may trigger long-term pathophysiological complications such as obesity and cardiovascular disease. Metabolic programming leads to organs and tissues modifications, including adipose tissue, with increased lipogenesis, production of inflammatory cytokines, and decreased glucose uptake. However, stem cells participation in adipose tissue dysfunctions triggered by overfeeding during lactation has not been elucidated. Therefore, this study was the first to evaluate the effect of metabolic programming on adipose mesenchymal stem cells (ASC) from mice submitted to overfeeding during lactation, using the litter reduction model. Cells were evaluated for proliferation capacity, viability, immunophenotyping, and reactive oxygen species (ROS) production. The content of UCP-2 and PGC1-α was determined by Western Blot. ASC differentiation potential in adipogenic and osteogenic environments was also evaluated, as well the markers of adipogenic differentiation (PPAR-γ and FAB4) and osteogenic differentiation (osteocalcin) by RT-qPCR. Results indicated that neonatal overfeeding does not affect ASC proliferation, ROS production, and viability. However, differentiation potential and proteins related to metabolism were altered. ASC from overfed group presented increased adipogenic differentiation, decreased osteogenic differentiation, and also showed increased PGC1-α protein content and reduced UCP-2 expression. Thus, ASC may be involved with the increased adiposity observed in neonatal overfeeding, and its therapeutic potential may be affected.

Keywords

Adipose mesenchymal stem cells Obesity Metabolic programming Perinatal overfeeding Litter size reduction 

Notes

Acknowledgements

This work was supported by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Rio de Janeiro (FAPERJ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors declare that they have no conflict of interest enrolled in this study.

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest enrolled in this study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratório de Pesquisa em Células-Tronco, Departamento de Histologia e Embriologia, Instituto de BiologiaUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Laboratório de Biologia do Câncer, Departamento de Biofísica e Biometria, Instituo de BiologiaUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil

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