Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization
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Pericytes are essential to vascularization, but the purification and characterization of pericytes remain unclear. Smooth muscle actin alpha (α-SMA) is one maker of pericytes. The aim of this study is to purify the α-SMA positive cells from bone marrow and study the characteristics of these cells and the interaction between α-SMA positive cells and endothelial cells. The bone marrow stromal cells were harvested from α-SMA-GFP transgenic mice, and the α-SMA-GFP positive cells were sorted by FACS. The proliferative characteristics and multilineage differentiation ability of the α-SMA-GFP positive cells were tested. A 3-D culture model was then applied to test their vascularization by loading α-SMA-GFP positive cells and endothelial cells on collagen-fibronectin gel. Results demonstrated that bone marrow stromal cells are mostly α-SMA-GFP positive cells which are pluripotent, and these cells expressed α-SMA during differentiation. The α-SMA-GFP positive cells could stimulate the endothelial cells to form tube-like structures and subsequently robust vascular networks in 3-D culture. In conclusion, the bone marrow derived pluripotent cells are pericytes and can contribute to vascularization.
KeywordsBone marrow stromal cells Pericytes Multilineage differentiation Endothelial cells Vascularization.
This work was funded by the Anthony and Constance Franchi Fund for Pediatric Orthopaedics at the MassGeneral Hospital for Children, The Peabody Foundation Inc., the National Natural Science Foundation of China (30801304), Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD 200977) and Program for New Century Excellent Talents in University (NCET-08-0373).
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