Capsaicin And Genistein Override The Action Of Obestatin To Decrease Lipid Accumulation In 3T3-L1 Cells
- 124 Downloads
Satiety peptides convey information about short-term energy reserves in the gut to the hypothalamus and aid in regulation of appetite and food intake. Obestatin is one such gastro peptide that has been shown to upregulate glycerolipid metabolism and PPARγ signalling. Obestatin brings about moderate reduction in circulating and stored triglyceride levels and reduction in gain in body weight in mice. We wanted to test whether obestatin could be further potentiated by co-administration with nutraceuticals genistein and capsaicin that are well known to reduce triglyceride levels. Hence, we chose to administer the compounds individually and pair-wise with obestatin in 3T3-L1 cells at concentrations of 200 nM obestatin, 10 µM capsaicin and 100 µM genistein. When treated along with induction of differentiation, both capsaicin and genistein in combination with obestatin reduced triglyceride levels in 3T3-L1 cells by 25 and 20%, respectively, when accessed on day 14 after induction. The combined administrations were dominated by the effect of the nutraceuticals and showed the same effect as of capsaicin or genistein. Upregulation of Fatty acid synthase (Fasn) and Adipose triglyceride lipase (Atgl/Pnpla2) by obestatin were reversed by both capsaicin and genistein. However, their ability to upregulate Peroxisome proliferation activating receptor gamma (Pparγ), Hormone sensitive lipase (Hsl), Lipoprotein lipase (Lpl) were retained while upregulation of Uncoupling protein 1 (Ucp1) by capsaicin was unchanged upon co-administration. Over expression of the lipases and UCP1 in case of capsaicin could be resulting in net lowering of lipid accumulation in the cells.
KeywordsCapsaicin Obesity Obestatin Genistein 3T3-L1cells
This work was financially supported under the BSC-0202 (WELFO) project of CSIR-CFTRI, funded by the Council of Scientific & Industrial Research, India (CSIR). MSKR would like to acknowledge CSIR for providing fellowship (31/005(0514)/2012-EMR-1).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 14.Kim, H. K., Nelson-Dooley, C., Della-Fera, M. A., Yang, J. Y., Zhang, W., Duan, J., Hartzell, D. L., Hamrick, M. W., Baile, C. A. (2006). Genistein Decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice. The Journal of Nutrition, 136(2), 409–414.Google Scholar
- 17.Ruiz-ojeda, F. J., Rupérez, A. I., Gomez-llorente, C., Gil, A., & Aguilera, C. M. (2016). Cell Models and their application for studying adipogenic differentiation in relation to obesity: a review. Int. J. Mol. Sci., 17(1040), 1–26.Google Scholar
- 20.Folch, J., Lees, M., & Sloane Steyanly, G. H. (1969). A Simple method for isolation and purification of total lipides from animal tissues. The Journal of Biological Chemistry, 4, 273–279. April.Google Scholar
- 21.Lowry, O. H., Rosebrough, N. J., Farr, A., & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry, 193, 265–275.Google Scholar
- 25.Stoops, J. K., Arslanian, M. J., Oh, Y. H., Aune, K. C., Thomas, C., Wakil, S. J., & Wakil, S. J. (2014). Presence of Two polypeptide chains comprising fatty acid synthetase. Proceedings of the National Academy of Sciences of the United States of America, 72(5), 1940–1944.Google Scholar
- 27.Elbrecht, A., Chen, Y., Cullinan, C. A., Hayes, N., Leibowitz, M. D., Moller, D. E., & Berger, J. (2000). Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors g 1 and g 2. Biochemical and Biophysical Research Communications, 437(224), 431–437.Google Scholar
- 31.Kozak, L. P., & Anunciado-Koza. (2009). UCP1: its involvement and utility in obesity. The International Journal of Obesity, 32(Suppl 7), S32–S38.Google Scholar
- 32.Reddy, M. S. K., & Manjappara, U. V. (2019). Capsaicin but not genistein influences modulation of lipid parameters by obestatin in DIO-C57BL/6 mice. International Journal of Peptide Research and Therapeutics. https://doi.org/10.1007/s10989-019-09811-9.