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Cell Biochemistry and Biophysics

, Volume 77, Issue 1, pp 61–67 | Cite as

Antiproliferative Effect of a Novel 4,4’-Disulfonyldiarylidenyl Piperidone in Human Colon Cancer Cells

  • Anjali M. Prabhat
  • M. Lakshmi Kuppusamy
  • Balázs Bognár
  • Tamás Kálai
  • Kálmán Hideg
  • Periannan KuppusamyEmail author
Original Paper

Abstract

The synthesis and antiproliferative effect of a novel curcumin analog, 4,4’-disulfonyldiarylidenyl piperidone, are reported. The design of the molecule is based on the fusion of an antiproliferative segment, namely diarylidenyl piperidone (DAP), with N-hyroxypyrroline, which is known to metabolically convert to nitroxide and protect healthy cells. Cellular uptake, metabolic conversion, cytotoxicity and antiproliferative effect of the DAP derivative against HCT-116 human colon cancer cells have been determined. Based on cell viability and proliferation assays as well as western-blot analysis of major transcription factors and inhibitory proteins, it is determined that the DAP compound is cytotoxic by inhibiting cell survival and proliferation pathways. The findings may have important implications in the design and development of effective anticancer agents.

Keywords

Disulfonyldiarylidenyl piperidone Colon cancer Rreactive oxygen species Nitroxide 

Notes

Acknowledgements

This work was supported by the Hungarian Research Fund Grant OTKA FK 124331 and GINOP 2.3.2-15-2016-00049.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Radiology and Medicine, Norris Cotton Cancer Center, Geisel School of MedicineDartmouth CollegeLebanonUSA
  2. 2.Institute of Organic and Medicinal Chemistry, Medical SchoolUniversity of PécsPécsHungary
  3. 3.Szentágothai Research CentreIfjusag st 20. H-7624PécsHungary

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