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Superoxide Formation in Cardiac Mitochondria and Effect of Phenolic Antioxidants

  • Arina L. Dudylina
  • Marina V. Ivanova
  • Konstantin B. Shumaev
  • Enno K. Ruuge
Original Paper

Abstract

Since mitochondria are the main cellular source of reactive oxygen species, it is important to study the effect of dietary phenolic compounds on the level of ROS in these organelles. Using the EPR spectroscopy and TIRON probe, the ability of the investigated phenols (quercetin, rutin, caffeic acid, curcumin, and resveratrol) to scavenge superoxide anion radicals generated by isolated heart mitochondria of Wistar rats under variable oxygen partial pressure was studied. It was shown that during a 10 min incubation, caffeic acid in concentrations of 10–500 μM most effectively scavenged superoxide radicals formed in the complex III of the mitochondrial respiratory chain. A comparable antioxidant effect of rutin under these experimental conditions was observed at higher concentrations of 1–10 mM. The antioxidant activity of quercetin in the concentration range of 10–500 μM during the first minutes of incubation was higher than that of caffeic acid. Of the phenolic compounds studied, curcumin had the least effect on the superoxide radicals.

Keywords

Reactive oxygen species Heart mitochondria Hypoxia Phenolic compounds Antioxidants EPR spectroscopy 

Abbreviations

Caf-OH

caffeic acid (3,4-dihydroxycinnamic acid)

Cur

curcumin ((1,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione)

LiPc

lithium phthalocyanine

LDL

low density lipoprotein

O2•−

superoxide anion radical

Qu

quercetin (3,3',4',5,7-pentahydroxyflavone)

ROS

reactive oxygen species

Rsv

resveratrol (3,5,4'-trihydroxy-trans-stilbene)

Rut

rutin (vitamin P)

TEMPONE-15N-D16

4-oxo-2,2,6,6-tetramethylpiperidine-D16-1-oxyl-15N

TIRON

4,5-dihydroxybenzene-1,3-disulfonate, disodium salt

Notes

Acknowledgements

This work was supported by the Russian Foundation for Basic Research (grants 15-04-05211 and 18-015-00125).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of PhysicsLomonosov Moscow State UniversityMoscowRussia
  2. 2.National Medical Research Centre for CardiologyMoscowRussia
  3. 3.Bach Institute of Biochemistry, Research Centre of BiotechnologyRussian Academy of SciencesMoscowRussia

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