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Cell Biochemistry and Biophysics

, Volume 71, Issue 1, pp 481–489 | Cite as

Heat Shock Protein 90 Stimulates Rat Mesenchymal Stem Cell Migration via PI3K/Akt and ERK1/2 Pathways

  • Feng Gao
  • Xinyang Hu
  • Xiaojie Xie
  • Xianbao Liu
  • Jianan WangEmail author
Original Paper

Abstract

The objective of this study was to determine the role of Hsp90α in regulating the migration of mesenchymal stem cells (MSCs) and to investigate the underlying mechanisms of this effect. MSCs migration was assessed by wound healing assay and transwell migration assay. Hsp90α expression was silenced in MSC by siRNA (sirHsp90α). The activity of secreted metalloproteases MMP-2 and MMP-9, and their expression levels in MSC were evaluated using gelatin zymography, Western blot analysis and real-time PCR. Gene expression of VCAM-1 and CXCR4 cytokines was evaluated by real-time PCR. Akt and ERK activity were analyzed by Western blotting using antibodies against phosphorylated forms of these proteins. Treatment with Hsp90α significantly enhanced MSC migration, and this effect was blocked by transfecting MSC with sirHsp90α. Treating the cells with recombinant human Hsp90α (rhHsp90α) enhanced gene expression and protein levels of MMP-2 and MMP-9, as well as their secretion and activity. MSC incubated with rhHsp90α exhibited increased gene expression of CXCR4 and VCAM-1. Finally, the levels of phosphorylated Akt and Erk were markedly increased by rhHsp90α treatment. These findings indicate that Hsp90α promotes MSCs migration via PI3K/Akt and ERK signaling pathways, and that this effect is possibly mediated by MMPs, SDF-1/CXCR4 pathway, and VCAM-1.

Keywords

Heat shock protein Mesenchymal stem cell Migration Cytokines 

Notes

Acknowledgments

This project was supported by the National Natural Science Foundation of China (Nos. 30670868, 30770887, and 30770887/H0220), the Traditional Chinese Medicine of Zhejiang Province (No. 2014ZA005), and the Zhejiang Provincial Natural Science foundation of China (Y2100362) and Science Technology Department of Zhejiang Province (2010R10G2010160). China.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Feng Gao
    • 1
    • 2
    • 3
  • Xinyang Hu
    • 1
    • 2
  • Xiaojie Xie
    • 1
    • 2
  • Xianbao Liu
    • 1
    • 2
  • Jianan Wang
    • 1
    • 2
    Email author
  1. 1.Department of Cardiology, Second Affiliated HospitalZhejiang University College of MedicineHangzhouPeople’s Republic of China
  2. 2.Cardiovascular Key Laboratory of Zhejiang Province, Second Affiliated HospitalZhejiang University College of MedicineHangzhouPeople’s Republic of China
  3. 3.Department of CardiologyZhejiang Provincial People’s HospitalHangzhouPeople’s Republic of China

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