Suppression of LPS-Induced Hepato- and Cardiotoxic Effects by Pulicaria petiolaris via NF-κB Dependent Mechanism
- 28 Downloads
Recently, there is an increasing interest in searching for harmless natural products isolated from plant materials that can be used as beneficial dietary supplements and/or therapeutic drug candidates. The present study aimed to test the potential protective role of Pulicaria petiolaris (PP, Asteraceae) against hepatic and cardiotoxic effects associated with lipopolysaccharide (LPS) injection. PP was given orally for 5 days at two different doses before LPS injection. Results have shown that LPS induced remarkable hepatic and cardiac injurious effects in mice. Hepatic damage was evident through increased serum transaminases, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and activity. Estimation of high levels of serum creatine kinase-MB (CK-MB) and cardiac troponin I indicated cardiac damage. Histopathological examination of liver and heart confirmed the biochemical results. Increase in oxidative stress along with a depressed antioxidant status of liver and heart were observed in LPS-intoxicated animals. Furthermore, LPS induced activation of nuclear factor-κB (NF-κB) and subsequent elevation of inflammatory cytokines (TNF-α, IL-6). On the other hand, PP treatment successfully safeguards both organs against LPS-induced injury as indicated by the improvement of the biochemical and histopathological parameters. These results suggest that PP ameliorates LPS-induced hepatic and cardiac oxidative injurious effects via antioxidant and anti-inflammatory effects.
KeywordsPulicaria petiolaris Asteraceae Lipopolysaccharide Hepatotoxicity Cardiotoxicity NF-κB
Inducible nitric oxide synthase
- IL-1, IL-6, IL-8, and IL-12
Interleukins 1, 6, 8, and 12
Reactive oxygen species
Reactive nitrogen species
Tumor necrosis factor-α
The authors acknowledge the Deanship of Scientific Research (DSR), Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia, for assistance.
GAM and SRMI were responsible for the collection of the plant and preparation of the extract. DSE, NA, GAM, HA, and SRMI conceived and designed the research. DSE, NA, HA and ME conducted the experiments and analyzed data. DSE, GAM, HA, and SRMI wrote the manuscript. All authors read and approved the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest regarding the publication of this manuscript.
- 5.Zhang, W.-B., Zhang, H.-Y., Zhang, Q., Jiao, F.-Z., Zhang, H., Wang, L.-W., et al. (2017). Glutamine ameliorates lipopolysaccharide-induced cardiac dysfunction by regulating the toll-like receptor 4/mitogen-activated protein kinase/nuclear factor-kB signaling pathway. Experimental and Therapeutic Medicine, 14, 5825–5832.Google Scholar
- 6.Zhang, W. B., Zhang, H. Y., Zhang, Q., Jiao, F. Z., Zhang, H., Wang, L. W., et al. (2017). Glutamine ameliorates lipopolysaccharide-induced cardiac dysfunction by regulating the toll-like receptor 4/mitogen-activated protein kinase/nuclear factor-kB signaling pathway. Experimental and Therapeutic Medicine, 14, 5825–5832.Google Scholar
- 16.Yusufoglu, H. S., Foudah, A. I., Alam, A., & Soliman, G. A. (2016). Cardioprotective and nephroprotective activities of methanolic extracts from Pulicaria somalensis herbs against carbon tetrachloride induced toxicity in rats. Planta Medica, 82(S01), S1–S381.Google Scholar
- 20.Collenette S (1999) Wild Flowers of Saudi Arabia. National Commission for Wild life Conservation and Development (NCWCD) & Sheila Collenette, King Fahd National Library, King of Saudi Arabia, pp. 169.Google Scholar
- 29.Ajuwon, O. R., Oguntibeju, O. O., & Marnewick, J. L. (2014). Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress. BMC Complementary and Alternative Medicine, 14, 392.CrossRefGoogle Scholar
- 30.Raish, M., Ahmad, A., Alkharfy, K. M., Ahamad, S. R., Mohsin, K., Al-Jenoobi, F. I., et al. (2016). Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model. BMC Complementary and Alternative Medicine, 16, 501.CrossRefGoogle Scholar
- 31.Wu, H., Pang, H., Chen, Y., Huang, L., Liu, H., Zheng, Y., et al. (2018). Anti-inflammatory effect of a polyphenol-enriched fraction from Acalypha wilkesiana on lipopolysaccharide-stimulated RAW 264.7 macrophages and acetaminophen-induced liver injury in mice. Oxidative Medicine and Cellular Longevity, 2018, 17.Google Scholar
- 34.Sugimoto, K., Sakamoto, S., Nakagawa, K., Hayashi, S., Harada, N., Yamaji, R., et al. (2011). Suppression of inducible nitric oxide synthase expression and amelioration of lipopolysaccharide-induced liver injury by polyphenolic compounds in Eucalyptus globulus leaf extract. Food Chemistry, 125, 442–446.CrossRefGoogle Scholar
- 36.Kao, E. S., Hsu, J. D., Wang, C. J., Yang, S. H., Cheng, S. Y., & Lee, H. J. (2009). Polyphenols extracted from Hibiscus sabdariffa L. inhibited lipopolysaccharide-induced inflammation by improving antioxidative conditions and regulating cyclooxygenase-2 expression. Bioscience, Biotechnology, and Biochemistry, 73, 385–390.CrossRefGoogle Scholar