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Toxic Effects of Particulate Matter Derived from Dust Samples Near the Dzhidinski Ore Processing Mill, Eastern Siberia, Russia

  • Katherine E. Zychowski
  • Abigail Wheeler
  • Bethany Sanchez
  • Molly Harmon
  • Christina R. Steadman Tyler
  • Guy Herbert
  • Selita N. Lucas
  • Abdul-Mehdi Ali
  • Sumant Avasarala
  • Nitesh Kunda
  • Paul Robinson
  • Pavan Muttil
  • Jose M. Cerrato
  • Barry Bleske
  • Olga Smirnova
  • Matthew J. CampenEmail author
Article
  • 36 Downloads

Abstract

Ambient particulate matter (PM) is associated with increased mortality and morbidity, an effect influenced by the metal components of the PM. We characterized five sediment samples obtained near a tungsten–molybdenum ore-processing complex in Zakamensk, Russia for elemental composition and PM toxicity with regard to pulmonary, vascular, and neurological outcomes. Elemental and trace metals analysis of complete sediment and PM10 (the respirable fraction, < 10 µm mass mean aerodynamic diameter) were performed using inductively coupled plasma optical emission spectrometry (ICP-OES) and mass spectrometry (ICP-MS). Sediment samples and PM10 consisted largely of silicon and iron and silicon and sodium, respectively. Trace metals including manganese and uranium in the complete sediment, as well as copper and lead in the PM10 were observed. Notably, metal concentrations were approximately 10 × higher in the PM10 than in the sediment. Exposure to 100 µg of PM10 via oropharyngeal aspiration in C56BL/6 mice resulted in pulmonary inflammation across all groups. In addition, mice exposed to three of the five PM10 samples exhibited impaired endothelial-dependent relaxation, and correlative analysis revealed associations between pulmonary inflammation and levels of lead and cadmium. A tendency for elevated cortical ccl2 and Tnf-α mRNA expression was induced by all samples and significant upregulation was noted following exposure to PM10 samples Z3 and Z4, respectively. Cortical Nqo1 mRNA levels were significantly upregulated in mice exposed to PM10 Z2. In conclusion, pulmonary exposure to PM samples from the Zakamensk region sediments induced varied pulmonary and systemic effects that may be influenced by elemental PM composition. Further investigation is needed to pinpoint putative drivers of neurological outcomes.

Keywords

Cardiovascular Toxicology Neuroinflammation Particulate matter Respiratory toxicology PM10 

Notes

Acknowledgements

This work has been supported by RFBR (Grant No. 16-05-01041) and NIEHS (Grant No. ES026673). Katherine E. Zychowski received funding support from NIGMS (Grant No. K12GM088021) through the ASERT-IRACDA program at UNM and NIEHS (Grant No. K99ES029104). We thank Dr. Jesse Denson for editing this manuscript. A special thank you to Vladimir Belogolovov for collection of the dust samples. The BRO-Baikal is a civil society focused on the protection of Lake Baikal and its tributaries based in Ulan-Ude, the capital of the Republic of Buryatia, Russia. Their US partner is the Southwest Research and Information Center (SRIC), is focused on human and natural resource impacts of mining including the prevention of air and water contamination from residual mining waste and remediation of mines in the arid southwestern US. This fantastic collaboration made this work possible.

Supplementary material

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Supplementary material 1 (DOCX 19 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Katherine E. Zychowski
    • 1
  • Abigail Wheeler
    • 1
  • Bethany Sanchez
    • 1
  • Molly Harmon
    • 1
  • Christina R. Steadman Tyler
    • 2
  • Guy Herbert
    • 1
  • Selita N. Lucas
    • 1
  • Abdul-Mehdi Ali
    • 3
  • Sumant Avasarala
    • 3
  • Nitesh Kunda
    • 1
  • Paul Robinson
    • 4
  • Pavan Muttil
    • 1
  • Jose M. Cerrato
    • 3
  • Barry Bleske
    • 5
  • Olga Smirnova
    • 6
  • Matthew J. Campen
    • 1
    Email author
  1. 1.Department of Pharmaceutical SciencesUniversity of New Mexico-Health Sciences CenterAlbuquerqueUSA
  2. 2.Bioscience DivisionLos Alamos National LaboratoriesLos AlamosUSA
  3. 3.Department of Civil EngineeringUniversity of New MexicoAlbuquerqueUSA
  4. 4.Southwest Research and Information CenterAlbuquerqueUSA
  5. 5.Pharmacy Practice and Administrative SciencesUniversity of New Mexico-Health Sciences CenterAlbuquerqueUSA
  6. 6.Geological Institute, Siberian BranchRussian Academy of SciencesMoscowRussia

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