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Cardiovascular Toxicology

, Volume 19, Issue 1, pp 48–55 | Cite as

Long-Term IL-2 Incubation-Induced L-type Calcium Channels Activation in Rat Ventricle Cardiomyocytes

  • Tatiana Filatova
  • Vadim Mitrokhin
  • Olga Kamkina
  • Irina Lovchikova
  • Mitko MladenovEmail author
  • Andre Kamkin
Article

Abstract

The following study examined the impact of IL-2 on Ca2+ channel activity in the event of several hours’ incubation in IL-2. The right ventricle free wall for action potential measurements was isolated and perfused with Tyrode solution. The whole-cell voltage clamp experiments were performed on enzymatically isolated single cardiomyocytes. The whole-cell voltage clamp recording of Ca2+ currents was performed using the Cs+-based pipette and bath solutions. The protocol with depolarizing prepulse (− 40 mV) was used to inactivate both Na+ current and Ca2+T-type current. The L-type Ca2+ current was elicited by a series of 250 ms depolarizing square pulses with 10 mV increments. At the 15th minute of continuous recording, the peak density at 0 mV was − 3.036 ± 0.3015 pA/pF under IL-2 and − 3.008 ± 0.3452 pA/pF in control conditions. The IL-2 in moderate concentration (1 ng/mL) has no acute effects on ICa.L in rat ventricular cells. In contrast, to the lack of acute effects, the long-term incubation with IL-2 (2 h or more) produced a prominent enhancement of Ca2+L-type current. In rat, ventricular myocardium IL-2 (1 ng/mL) produced a very gradual prolongation of subendocardial APs which reached a maximal extent after 3–4 h of treatment. The patch clamp study shows an IL-2-induced ICa.L current activation, while the action potential studies on multicellular ventricular preparations suggest an IL-2-induced L-type Ca2+ channel participation in the development of AP.

Keywords

Interleukin 2 L-type Ca2+ current (ICa.LAction potential duration (APD) Right ventricle Heart Rat 

Notes

Acknowledgements

This work was supported by the Russian Science Foundation.

Author Contributions

All authors contributed toward data analysis, drafting, and revising the paper and agree to be accountable for all aspects of the work.

Funding

The present study was supported by a grant from the Russian Science Foundation (Grant No. 16-14-10372).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Tatiana Filatova
    • 1
    • 2
  • Vadim Mitrokhin
    • 1
  • Olga Kamkina
    • 1
  • Irina Lovchikova
    • 1
  • Mitko Mladenov
    • 1
    • 3
    Email author
  • Andre Kamkin
    • 1
  1. 1.Department of Fundamental and Applied PhysiologyRussian National Research Medical UniversityMoscowRussia
  2. 2.Department of Human and Animal PhysiologyBiological Faculty of the Moscow State UniversityMoscowRussia
  3. 3.Faculty of Natural Sciences and Mathematics, Institute of Biology“Ss. Cyril and Methodius” UniversitySkopjeMacedonia

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