Evaluation of Cadmium Levels in Dental Calculus of Male Oral SCC Patients with Betel-Quid Chewing in Hunan Province of China
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Cadmium (Cd) is a widespread trace toxic heavy metal with long biological half-life and may induce higher risk of cancer on multiple organs of human body. Recent studies have confirmed that dental calculus has enormous potential for investigation of exposure to Cd in the human mouth by acting as a time capsule. We aimed to examine relationship between Cd levels in dental calculus due to betel-quid chewing and risk of oral cancer. This study included 85 male oral squamous cell carcinoma (SCC) cases with betel-quid chewing and smoking as observation subjects (group A) and 67 healthy people with smoking but without betel-quid chewing as control subjects (group B) in Hunan province of Mainland China. Cd levels in calcified dental calculus samples from all participants were measured by inductively coupled plasma mass spectrometry (ICP-MS). The results of this study indicated that cadmium levels in dental calculus were significantly higher in male oral SCC patients with betel-quid chewing and smoking than that in healthy individuals without habit of betel-quid chewing and with smoking (p < 0.0001). This study gives some evidence to support that there may be a positive relationship between cadmium in dental calculus due to betel-quid chewing and risk of oral SCC.
KeywordsCadmium Dental calculus Betel quid Oral squamous cell carcinoma Carcinogens ICP-MS
The authors would like to thank Dr. and Prof. Tian-Ting Zhang, at The Third Xiangya Hospital, Changsha, Hunan, PR China, for encouragement and support for this study.
Dr. Bo Zhang, Dr. Xiaodan Tan, and Dr. Xifan He contributed equally to this work. Dr. Bo Zhang, Dr. Xiaodan Tan, and Dr. Kunlun Zhang participated in the study design. Dr. Bo Zhang, Dr. Xifan He, Dr. Hanlin Yang, and Dr. Yuehui Wang collected dental calculus samples. Dr. Bo Zhang, Dr. Xiaodan Tan, and Dr. Kunlun Zhang performed experiments. Dr. Bo Zhang analyzed the data. Dr. Bo Zhang and Dr. Xiaodan Tan wrote the paper. All authors read and approved the paper.
This study was supported by Hunan S & T Project of China Hunan provincial science and technology department (Grant No. 2014FJ3032).
Compliance with Ethical Standards
This study was approved by the Ethics Committee of Hunan Provincial People’s Hospital, and informed and signed consent was obtained from all participants
Conflict of Interest
The authors declare that they have no conflict of interest related to this study.
- 2.Dobrowolski R, Klatka J, Brodnjak-Voncina D, Trojanowska A, Mysliwiec D, Ostrowski J, Remer M (2014) Chemometric methods for studying the relationships between trace elements in laryngeal cancer and healthy tissues. Biol Trace Elem Res 159:107–114. https://doi.org/10.1007/s12011-014-0013-9 CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Jepsen S, Deschner J, Braun A, Schwarz F, Eberhard J (2011) Calculus removal and the prevention of its formation. Periodontol 2000 55:167–188. https://doi.org/10.1111/j.1600-0757.2010.00382.x
- 7.Akcali A, Lang NP (2018) Dental calculus: the calcified biofilm and its role in disease development. Periodontol 2000 76:109–115. https://doi.org/10.1111/prd.12151
- 9.Herman M, Golasik M, Piekoszewski W, Walas S, Napierala M, Wyganowska-Swiatkowska M, Kurhanska-Flisykowska A, Wozniak A, Florek E (2016) Essential and toxic metals in oral fluid-a potential role in the diagnosis of periodontal diseases. Biol Trace Elem Res 173:275–282. https://doi.org/10.1007/s12011-016-0660-0 CrossRefPubMedPubMedCentralGoogle Scholar
- 11.Afridi HI, Talpur FN, Kazi TG, Brabazon D (2015) Effect of trace and toxic elements of different brands of cigarettes on the essential elemental status of Irish referent and diabetic mellitus consumers. Biol Trace Elem Res 167:209–224. https://doi.org/10.1007/s12011-015-0308-5 CrossRefPubMedGoogle Scholar
- 12.Yaprak E, Yolcubal İ, Sinanoğlu A, Doğrul-Demiray A, Guzeldemir-Akcakanat E, Marakoğlu I (2017) High levels of heavy metal accumulation in dental calculus of smokers: a pilot inductively coupled plasma mass spectrometry study. J Periodontal Res 52:83–88. https://doi.org/10.1111/jre.12371 CrossRefPubMedGoogle Scholar
- 15.Costa de Almeida GR, Pereira Saraiva Mda C, Barbosa F Jr, Krug FJ, Cury JA, Rosário de Sousa Mda L, Rabelo Buzalaf MA, Gerlach RF (2007) Lead contents in the surface enamel of deciduous teeth sampled in vivo from children in uncontaminated and in lead-contaminated areas. Environ Res 104:337–345. https://doi.org/10.1016/j.envres.2007.03.007 CrossRefPubMedGoogle Scholar
- 16.Zhang X, Reichart PA (2007) A review of betel quid chewing, oral cancer and precancer in Mainland China. Oral Oncol 43:424–430. https://doi.org/10.1016/j.oraloncology.2007.01.020 CrossRefPubMedGoogle Scholar
- 20.Manoharan S, Nagaraja V, Eslick GD (2014) Ill-fitting dentures and oral cancer: a meta-analysis. Oral Oncol 50:1058–1061. https://doi.org/10.1016/j.oraloncology.2014.08.002 CrossRefPubMedGoogle Scholar
- 21.Anantharaman D, Marron M, Lagiou P, Samoli E, Ahrens W, Pohlabeln H, Slamova A, Schejbalova M, Merletti F, Richiardi L, Kjaerheim K, Castellsague X, Agudo A, Talamini R, Barzan L, Macfarlane TV, Tickle M, Simonato L, Canova C, Conway DI, McKinney PA, Thomson P, Znaor A, Healy CM, McCartan BE, Hashibe M, Brennan P, Macfarlane GJ (2011) Population attributable risk of tobacco and alcohol for upper aerodigestive tract cancer. Oral Oncol 47:725–731. https://doi.org/10.1016/j.oraloncology.2011.05.004 CrossRefPubMedGoogle Scholar
- 22.Gaur S, Agnihotri R (2018) Health effects of trace metals in electronic cigarette aerosols-a systematic review. Biol Trace Elem Res. https://doi.org/10.1007/s12011-018-1423-x
- 23.Ray JG, Ghosh R, Mallick D, Swain N, Gandhi P, Ram SS, Selvaraj S, Rathore A, Mathummal S, Chakraborty A (2011) Correlation of trace elemental profiles in blood samples of Indian patients with leukoplakia and oral submucous fibrosis. Biol Trace Elem Res 144:295–305. https://doi.org/10.1007/s12011-011-9091-0 CrossRefPubMedGoogle Scholar
- 28.Gašparík J, Binkowski ŁJ, Jahnátek A, Šmehýl P, Dobiaš M, Lukáč N, Błaszczyk M, Semla M, Massanyi P (2017) Levels of metals in kidney, liver, and muscle tissue and their influence on the fitness for the consumption of wild boar from western Slovakia. Biol Trace Elem Res 177:258–266. https://doi.org/10.1007/s12011-016-0884-z CrossRefPubMedGoogle Scholar
- 36.Warinner C, Rodrigues JF, Vyas R, Trachsel C, Shved N, Grossmann J, Radini A, Hancock Y, Tito RY, Fiddyment S, Speller C, Hendy J, Charlton S, Luder HU, Salazar-García DC, Eppler E, Seiler R, Hansen LH, Castruita JA, Barkow-Oesterreicher S, Teoh KY, Kelstrup CD, Olsen JV, Nanni P, Kawai T, Willerslev E, von Mering C, Lewis CM Jr, Collins MJ, Gilbert MT, Rühli F, Cappellini E (2014) Pathogens and host immunity in the ancient human oral cavity. Nat Genet 46:336–344. https://doi.org/10.1038/ng.2906 CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Velsko LM, Overmyer KA, Speller C, Klaus L, Collins MJ, Loe L, Frantz LAF, Sankaranarayanan K, Lewis CM Jr, Martinez JBR, Chaves E, Coon JJ, Larson G, Warinner C (2017) The dental calculus metabolome in modern and historic samples. Metabolomics 13(11):134. https://doi.org/10.1007/s11306-017-1270-3 CrossRefPubMedPubMedCentralGoogle Scholar
- 41.Warnakulasuriya S (2009) Global epidemiology of oral and oropharyngeal cancer. Oral Oncol 45:309–316. https://doi.org/10.1016/j.oraloncology.2008.06.002 CrossRefPubMedGoogle Scholar
- 42.Bagan J, Sarrion G, Jimenez Y (2010) Oral cancer: clinical features. Oral Oncol 46:414–417. https://doi.org/10.1016/j.oraloncology.2010.03.009 CrossRefPubMedGoogle Scholar
- 45.Mizrachi A, Migliacci JC, Montero PH, McBride S, Shah JP, Patel SG, Ganly I (2018) Neck recurrence in clinically node-negative oral cancer: 27-year experience at a single institution. Oral Oncol 78:94–101. https://doi.org/10.1016/j.oraloncology.2018.01.020 CrossRefPubMedGoogle Scholar
- 47.Klotz K, Weistenhöfer W, Drexler H (2013) Determination of cadmium in biological samples. Met Ions Life Sci 11:85–98. https://doi.org/10.1007/978-94-007-5179-84 Review
- 49.Kiziler AR, Aydemir B, Onaran I, Alici B, Ozkara H, Gulyasar T, Akyolcu MC (2007) High levels of cadmium and lead in seminal fluid and blood of smoking men are associated with high oxidative stress and damage in infertile subjects. Biol Trace Elem Res 120:82–91. https://doi.org/10.1007/s12011-007-8020-8 CrossRefPubMedGoogle Scholar
- 52.Alhasmi AM, Gondal MA, Nasr MM, Shafik S, Habibullah YB (2015) Detection of toxic elements using laser-induced breakdown spectroscopy in smokers’ and nonsmokers’ teeth and investigation of periodontal parameters. Appl Opt 54:7342–7349. https://doi.org/10.1364/AO.54.007342 CrossRefPubMedGoogle Scholar