Treatment with Lead Chloride During Pregnancy and the Postnatal Period Alters Cell Proliferation and Immune Function in Swiss Albino Mice
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In the current study, we investigated the effect of lead chloride (PbCl2) administration (50 and 100 ppm) on organ and body weight as well as its bioaccumulation during pregnancy and the postnatal period in mice. We showed that lead has no effect on the body weight of mice. However, spleen weight is affected by the two doses of PbCl2 while liver and kidney weights are altered only by the 100-ppm dose. Inductively coupled plasma atomic emission spectrometry (ICP-AES) analysis showed that lead accumulates in the blood, spleen, and thymus. Both doses of PbCl2 significantly reduced splenocyte and thymocyte cell counts after stimulation with lipopolysaccharide (LPS) and phytohemagglutinin A (PHA), respectively. On the other hand, we showed that the levels of Th1 cytokines (interleukin-2 (IL-2), interferon gamma (IFN-γ)), and tumor necrosis factor alpha (TNF-α) were reduced in the serum of mice treated with PbCl2 in a dose-dependent manner, as measured by ELISA. The levels of interleukin-4 (IL-4) and interleukin-10 (IL-10) were very low in untreated mice and were also reduced by treatment with PbCl2. The levels of IL-2, IFN-γ, IL-4, IL-10, and TNF-α secretion differentially decreased in LPS-stimulated splenocytes in lead-treated mice. Using PHA-stimulated thymocytes, we observed a reduction in the levels of IL-2, IL-4, IL-10, and TNF-α in the PbCl2-treated groups. However, IFN-γ concentration in the supernatant of these cells was not decreased when mice were treated with 50 ppm of lead.
KeywordsPbCl2 Immunomodulation Cell proliferation Th1/Th2 balance Cytokines ELISA sandwich
Tumor necrosis factor alpha
The authors gratefully acknowledge the expert technical assistance of Pr. Mohamed EL-Baghdadi (Faculty of Sciences and Techniques, Sultan Moulay Slimane University, Beni Mellal, Morocco) and Dr. Amorette Barber (Associate Professor of Biology, Longwood University, Farmville, VA, USA), for reading and editing the manuscript.
This work was supported by the “Foundation Lalla Salma: Cancer Prevention and Treatment” Rabat. Morocco. Project 9/2013.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that there are no conflicts of interest.
- 2.World Health Organization (2010) Action is needed on chemicals of major public health concern. 20 Avenue Appia, CH-1211 Geneva-27, Switzerland. https://www.who.int/ipcs/features/10chemicals_en.pdf?ua=1
- 3.Lawrence DA (1981) In vivo and in vitro effects of lead on humoral and cell-mediated immunity. 31:9Google Scholar
- 6.Queiroz MLS, Almeida M, Gallão MI, Höehr NF (1993) Defective neutrophil function in workers occupationally exposed to lead. Pharmacol Toxicol 72:73–77. https://doi.org/10.1111/j.1600-0773.1993.tb00293.x CrossRefPubMedGoogle Scholar
- 7.McCabe MJ, Lawrence DA (1990) The heavy metal lead exhibits B cell-stimulatory factor activity by enhancing B cell Ia expression and differentiation. J Immunol 145:8Google Scholar
- 9.Ikenaka Y, Nakayama SMM, Muroya T et al (2012) Effects of environmental lead contamination on cattle in a lead/zinc mining area: changes in cattle immune systems on exposure to lead in vivo and in vitro. Environ Toxicol Chem 31:2300–2305. https://doi.org/10.1002/etc.1951 CrossRefPubMedGoogle Scholar
- 17.Sarasua SM, Vogt RF, Henderson LO et al (2010) Serum immunoglobulins and lymphocyte subset distributions in children and adults living in communities assessed for lead and cadmium exposure. J Toxicol Environ Health A 60:1–15Google Scholar
- 22.Aisemberg J, Vercelli CA, Bariani MV et al (2013) Progesterone is essential for protecting against LPS-induced pregnancy loss. LIF as a potential mediator of the anti-inflammatory effect of progesterone. PLoS ONE 8:e56161. https://doi.org/10.1371/journal.pone.0056161 CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Grara N, Boucenna M, Atailia A et al (2012) Etude expérimentale de la bioaccumulation des éléments traces métalliques Cd/Cu/Zn et Pb chez l’escargot Helix aspersa. Bull L’Institut Sci Rabat 2:183–187Google Scholar
- 27.Salman NM (2017) Accumulation of lead in the tissues and effects on growth rate of freshwater Cyprinus carpio. J Entomol Zool Stud 5:1499–1502Google Scholar
- 29.Raza B, Javed M, Ambreen F, Latif F (2016) Toxic effect of lead chloride on antioxidant enzyme in the liver and kidney of fish. J Bioresour Manag 3:9Google Scholar
- 39.Sharma R, Kantwa SM (2011) Effects of Vitamin C on Lead Induced Developing Thymus in Mice: A review. Univers J Environ Res Technol 1:91–102Google Scholar
- 43.Sen A, Heredia N, Senut M-C, et al (2015) Multigenerational epigenetic inheritance in humans: DNA methylation changes associated with maternal exposure to lead can be transmitted to the grandchildren. Sci Rep 5:1–10. https://doi.org/10.1038/srep14466
- 49.Kamińska T, Filar J, Madej E, et al (1998) Modification of bovine interferon and tumor necrosis factor production by lead in vivo and in vitro. Arch ImmunolTherExp (Warsz) 46:323–328Google Scholar
- 50.Flohé SB, Brüggemann J, Herder C, et al Enhanced proinflammatory response to endotoxin after priming of macrophages with lead ions. J LeukocBiol 3:417–424Google Scholar
- 58.Friberg D, Bryant J, Shannon W, Whiteside TL (1994) In vitro cytokine production by normal human peripheral blood mononuclear cells as a measure of immunocompetence or the state of activation. ClinDiagn Lab Immunol 1:261–268Google Scholar