Contents of Chromium and Arsenic in Tea (Camellia sinensis L.): Extent of Transfer into Tea Infusion and Health Consequence

  • Tupu Barman
  • A. K. Barooah
  • B. C. Goswami
  • Nipom Sharma
  • Saumik Panja
  • Puja Khare
  • Tanmoy KarakEmail author


Tea (Camellia sinensis L.) is the most popular beverage in the world after water. Due to acidophilic nature of tea plant, it has inherent tendency to uptake metals/metalloids including the toxic ones from the soil which is of great concern worldwide. In this study, level of chromium (Cr) and arsenic (As) were assessed in four hundred ninety-seven (497) black tea samples collected from six tea growing regions of Assam and North Bengal, India. The average concentration of Cr and As in the tested black tea samples was 10.33 and 0.11 μg g−1, respectively. Since tea is consumed as a beverage, transfer of Cr and As from black tea to its hot water extract (also known as tea infusion) was also accessed. The amount of Cr and As determined in the tea infusion was much less (< 0.20 to 1.38 μg g−1 for Cr and < 3.60 to 34.79 μg kg−1 for As) than those in the black teas with the transfer rate up to 5.96% and 8.53%, respectively. The present study showed that values of hazard quotient were well below one suggesting that intake of Cr and As from consumption of five cups of tea equivalent to 10 g black tea would not impose any health hazard.


Arsenic Assam and North Bengal, India Black tea Chromium Infusion Hazard quotient 



The data of present work were collected as a part of Mr. Tupu Barman’s pre-PhD thesis at Tocklai Tea Research Institute, Jorhat, Assam, India. We acknowledge Tea Board of India, Ministry of Commerce, Govt. of India, under XI plan project on studies on heavy metals—Phase II—chromium and arsenic for the financial support in this work. The financial support provided by the programme entitled “DBT’s Overseas Associateship in respect of North Eastern Region (NER) through NER Biotechnology Programme Management Cell (NER-BPMC)” vide Department of Biotechnology (DBT) Sanction Order BT/20/NE/2011 dated 23.05.2018 for Dr. Tanmoy Karak is hereby duly acknowledged. Finally, we express our sincere thanks to two anonymous reviewers for their valuable suggestions to improve this manuscript.

Compliance with Ethical Standards

Conflict of Interest

There is no conflict of interest. The authors’ affiliations are shown on the cover page. The authors have sole responsibility for the writing and content of the paper.


  1. 1.
    Han WY, Shi YZ, Ma LF, Ruan YZ (2005) Arsenic, cadmium, chromium, cobalt, and copper in different types of Chinese tea. Bull Environ ContamToxicol 75:272–277CrossRefGoogle Scholar
  2. 2.
    Barua DN (2008) Science and practice in tea culture. Tea Research Association, Calcutta-JorhatGoogle Scholar
  3. 3.
    Karak T, Abollino O, Bhattacharyya P, Das KK, Paul RK (2011) Fractionation and speciation of arsenic in three tea gardens soil profiles and distribution of As in different parts of tea plant (Camellia sinensis L.). Chemosphere 85:948–960CrossRefGoogle Scholar
  4. 4.
    Karak T, Paul RK, Sonar I, Sanyal S, Ahmed KZ, Boruah RK, Das DK, Dutta AK (2014) Chromium in soil and tea (Camellia sinensis L.) infusion: does soil amendment with municipal solid waste compost make sense? Food Res Int 64:114–124CrossRefGoogle Scholar
  5. 5.
    Shen FM, Chen HW (2008) Element composition of tea leaves and tea infusions and its impact on health. Bull Environ Contam Toxicol 80:300–304CrossRefGoogle Scholar
  6. 6.
    Soomro MT, Zahir E, Mohiuddin S, Khan AN, Naqvi II (2008) Quantitative assessment of metals in local brands of tea in Pakistan. Pak J Biol Sci 11(2):285–289CrossRefGoogle Scholar
  7. 7.
    Gebretsadik DW, Chandravanshi BS (2010) Levels of metals in commercially available Ethiopian black teas and their infusions. Bull Chem Soc Ethiop 24:339–349CrossRefGoogle Scholar
  8. 8.
    Zheng H, Li JL, Li HH, Hu GC, Li HS (2014) Analysis of trace metals and perfluorinated compounds in 43 representative tea products from South China. J Food Sci 79(6):1123–1129CrossRefGoogle Scholar
  9. 9.
    Bıyık R, Tapramaz R (2010) Transition metal ions in black tea: an electron paramagnetic resonance study. Transition Met Chem 35:27–31CrossRefGoogle Scholar
  10. 10.
    Pękal A, Biesaga M, Pyrzynska K (2013) Trace metals and flavonoids in different types of tea. Food Sci Biotechnol l22(4):925–930CrossRefGoogle Scholar
  11. 11.
    Indian Council of Medical Research, New Delhi, ICMR bulletin (2003) Tea consumption on oxidative damage and cancer. In: Medappa N (ed), ICMR Offset Press, New Delhi, pp 33(1–2)Google Scholar
  12. 12.
    Ashraf W, Mian AA (2008) Levels of selected heavy metals in black tea varieties consumed in Saudi Arabia. Bull Environ Contam Toxicol 81:101–104CrossRefGoogle Scholar
  13. 13.
    Karak T, Bhagat RM (2010) Trace elements in tea leaves, made tea and tea infusion: a review. Food Res Int 43:2234–2252CrossRefGoogle Scholar
  14. 14.
    Salahinejad M, Aflaki F (2010) Toxic and essential mineral elements content of black tea leaves and their tea infusions consumed in Iran. Biol Trace Elem Res 134:109–117CrossRefGoogle Scholar
  15. 15.
    Nkansah MA, Opoku F, Ackumey AA (2016) Risk assessment of mineral and heavy metal content of selected tea products from the Ghanaian market. Environ Monit Assess 188(6):332CrossRefGoogle Scholar
  16. 16.
    Yaylali-Abanuz G, Tuysuz N (2009) Heavy metal contamination of soils and tea plants in the eastern Black Sea region, NE Turkey. Environ Earth Sci 59:131–144CrossRefGoogle Scholar
  17. 17.
    Li L, Fu QL, Achal V, Liu Y (2015) A comparison of the potential health risk of aluminium and heavy metals in tea leaves and tea infusion of commercially available green tea in Jiangxi, China. Environ Monit Assess 187(5):228CrossRefGoogle Scholar
  18. 18.
    Karak T, Paul RK, Kutu FR, Mehra A, Khare P, Dutta AK, Bora K, Boruah RK (2016) Comparative assessment of copper, iron, and zinc contents in selected Indian (Assam) and South African (Thohoyandou) tea (Camellia sinensis L.) samples and their infusion: a quest for health risks to consumer. Biol Trace Elem Res 175(2):475–487CrossRefGoogle Scholar
  19. 19.
    Food and Agriculture Organization -Intergovernmental Group on Tea (2018) 23rd session Current market situation and medium term outlook for tea to 2027 Document CCP:TE 18/CRS1 Accessed 30 Aug 2018
  20. 20.
    Tea board of India, Ministry of Commerce and Industry, Government of India Accessed 30 Aug 2018
  21. 21.
    Jin CW, Zheng SJ, He YF, Zhou GD, Zhou ZX (2005) Lead contamination in tea garden soils and factors affecting its bioavailability. Chemosphere 59:1151–1159CrossRefGoogle Scholar
  22. 22.
    Han WY, Zhao FZ, Shi YZ, Ma LF, Ruan JY (2006) Scale and causes of lead contamination in Chinese tea. Environ Pollut 139:125–132CrossRefGoogle Scholar
  23. 23.
    Memic M, Mahic D, Zero S, Muhic-Sarac T (2014) Comparison of different digestion methods of green and black tea at the Sarajevo market for the determination of the heavy metal content. Food Measure 8:149–154CrossRefGoogle Scholar
  24. 24.
    Habila M, Unsal YE, Alothman ZA, Shabaka A, Tuzen M, Soylak M (2015) Speciation of chromium in natural waters, tea, and soil with membrane filtration flame atomic absorption spectrometry. Anal Lett 48(14):2258–2271CrossRefGoogle Scholar
  25. 25.
    Karak T, Paul RK, Sonar I, Nath JR, Boruah RK, Dutta AK (2016) Nickel dynamics influenced by municipal solid waste compost application in tea (Camellia sinensis L.): a cup that cheers. Int J Environ Sci Technol 13(2):663–678CrossRefGoogle Scholar
  26. 26.
    Brzezicha-Cirocka J, Grembecka M, Szefer P (2016) Analytical assessment of bio- and toxic elements distribution in pu-erh and fruit teas in view of chemometric approach. Biol Trace Elem Res 174:240–250CrossRefGoogle Scholar
  27. 27.
    Mania M, Szynal T, Rebeniak M, Wojciechowska-Mazurek M, Starska K, Strzelecka A (2014) Human exposure assessment to different arsenic species in TEA. RoczPanstwZaklHig 65(4):281–286Google Scholar
  28. 28.
    Jin CW, He YF, Zhang K, Zhou GD, Shi JL, Zheng SJ (2005) Lead contamination in tea leaves and non-edaphic factors affecting it. Chemosphere 61:726–732CrossRefGoogle Scholar
  29. 29.
    Arpadjana S, Çelik G, Taskesen S, Güçer S (2008) Arsenic, cadmium and lead in medicinal herbs and their fractionation. Food Chem Toxicol 46:2871–2875CrossRefGoogle Scholar
  30. 30.
    Jin CW, Du ST, Zhang K, Lin XY (2008) Factors determining copper concentration in tea leaves produced at Yuyao county, China. Food Chem Toxicol 46:2054–2061CrossRefGoogle Scholar
  31. 31.
    Mandiwana KL, Panichev N, Panicheva S (2011) Determination of chromium(VI) in black, green and herbal teas. Food Chem 129:1839–1843CrossRefGoogle Scholar
  32. 32.
    Prkić A, Giljanović J, Petričević S, Brkljača M, Bralić M (2013) Determination of cadmium, chromium, copper, iron, lead, magnesium, manganese, potassium and zinc in mint tea leaves by electrothermal atomizer atomic absorption spectrometry in samples purchased at local supermarkets and marketplaces. Anal Lett 46:367–378CrossRefGoogle Scholar
  33. 33.
    Seenivasan S, Anderson TA, Muraleedharan N (2016) Heavy metal content in tea soils and their distribution in different parts of tea plants, Camellia sinensis (L).O.Kuntze. Environ Monit Assess 188(7):428CrossRefGoogle Scholar
  34. 34.
    Novotnik B, Zuliani T, Scancar J, Milacic R (2015) Content of trace elements and chromium speciation in neem powder and tea infusions. J Trace Elem Med Biol 31:98–106CrossRefGoogle Scholar
  35. 35.
    Novotnik B, Zuliani T, Scancar J, Milacic R (2013) Chromate in food samples : an artefact of wrongly applied analytical methodology? J Anal At Spectrom 28:558–566CrossRefGoogle Scholar
  36. 36.
    Milacic R, Scancar J (2017) Comment on recent article speciation of Cr in bread and breakfast cereals, published in food chemistry, 129, 1839-1843 by Mathebula, M. W., Mandiwana, K., & Panichev, N.: letter to the editor. Food Chem 254:78–79CrossRefGoogle Scholar
  37. 37.
    Han WY, Shi YZ, Ma LF, Ruan JY, Zhao FJ (2007) Effect of liming and seasonal variation on lead concentration of tea plant (Camellia sinensis (L.) O. Kuntze). Chemosphere 66:84–90CrossRefGoogle Scholar
  38. 38.
    El-Hadri F, Morales-Rubio A, Guardia MDL (2007) Determination of total arsenic in soft drinks by hydride generation atomic fluorescence spectrometry. Food Chem 105:1195–1200CrossRefGoogle Scholar
  39. 39.
    AOAC 18th edition, 2005, revision 2, 2007, Chapter 9, p. 22Google Scholar
  40. 40.
    Ferrara L, Montesanoa D, Senatore A (2001) The distribution of minerals and flavonoids in the tea plant (Camellia sinensis). II Farmaco 56(5–7):397–401CrossRefGoogle Scholar
  41. 41.
    Seenivasan S, Manikandan N, Muraleedharan NN (2008) Chromium contamination in black tea and its transfer into tea brew. Food Chem 106:1066–1069CrossRefGoogle Scholar
  42. 42.
    Li WH, Zhou HP, Li N, Wang SD, Liu XJ, Jin ZJ, Bu YZ, Liu ZX (2013) Chromium level and intake from Chinese made tea. Food Addit. Contam., Part B 6(4):289–293CrossRefGoogle Scholar
  43. 43.
    Narin CH, Turkoglu O, Soylak M, Dogan M (2004) Heavy metals in black tea samples produced in Turkey. Bull Environ ContamToxicol 72:844–849Google Scholar
  44. 44.
    Wróbel K, Wróbel K, Urbina EMC (2000) Determination of total aluminum, chromium, copper, iron, manganese and nickel and their fractions leached to the infusions of black tea, green tea, hibiscus sabdariffa, and ilexparaguariensis(mate) by ETA-AAS. Biol Trace Elem Res 78:271–280CrossRefGoogle Scholar
  45. 45.
    Zhong WS, Ren T, Zhao LJ (2016) Determination of Pb (Lead), cd (cadmium), Cr (chromium), Cu (copper), and Ni (nickel) in Chinese tea with high-resolution continuum source graphite furnace atomic absorption spectrometry. J Food Drug Anal 24:46–55CrossRefGoogle Scholar
  46. 46.
    Brzezicha-Cirocka J, Grembecka M, Szefer P (2016) Monitoring of essential and heavy metals in green tea from different geographical origins. Environ Monit Assess 188(3):183CrossRefGoogle Scholar
  47. 47.
    Al-Othman ZA, Yilmaz E, Sumayli HMT, Soylak M (2012) Evaluation of trace metals in tea samples from Jeddah and Jazan, Saudi Arabia by atomic absorption spectrometry. Bull Environ Contam Toxicol 89:1216–1219CrossRefGoogle Scholar
  48. 48.
    Lv HP, Lin Z, Tan JF, Guo L (2013) Contents of fluoride, lead, copper, chromium, arsenic and cadmium in Chinese Pu-erh tea. Food Res Int 53:938–944CrossRefGoogle Scholar
  49. 49.
    Shi YZ, Jin LM, Zhu YS (2007) The contents status quo and the cumulation characteristic of heavy metal in tea. China Tea 6:17–19Google Scholar
  50. 50.
    Yuan C, Gao E, He B, Jiang G (2007) Arsenic species and leaching characters in tea (Camellia sinensis). Food Chem Toxicol 45:2381–2389CrossRefGoogle Scholar
  51. 51.
    Karimi G, Hasanzadeh MK, Nili A, Khashayarmanesh Z, Samiei Z, Nazari F, Teimuri M (2008) Concentrations and health risk of heavy metals in tea samples marketed in IRAN. Pharmacologyonline 3:164–174Google Scholar
  52. 52.
    Rashid MH, Fardous Z, Chowdhury MAZ, Alam MK, Bari ML, Moniruzzaman M, Gan SH (2016) Determination of heavy metals in the soils of tea plantations and in fresh and processed tea leaves: an evaluation of six digestion methods. Chem Cent J 10:7CrossRefGoogle Scholar
  53. 53.
    Zhang L, Zhang J, Chen L, Liu T, Ma G, Liu X (2018) Influence of manufacturing process on the contents of iron, copper, chromium, nickel and manganese elements in crush, tear and curl black tea, their transfer rates and health risk assessment. Food Control 89:241–249CrossRefGoogle Scholar
  54. 54.
    Food and Nutrition Board, Institute of Medicine (2001) Dietary reference intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. National Academy Press, Washington, DC, pp 197–223Google Scholar
  55. 55.
    Evaluation of certain food additives and contaminants (Thirty-third report of the joint FAO/WHO) expert committee on food additives, 1989). WHO technical report series No:776Google Scholar

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Authors and Affiliations

  1. 1.Analytical Services DepartmentTocklai Tea Research InstituteJorhatIndia
  2. 2.Cotton UniversityGuwahatiIndia
  3. 3.Department of Civil, Environmental and Ocean EngineeringStevens Institute of TechnologyHobokenUSA
  4. 4.Agronomy-Soil DivisionCSIR-Central Institute of Medicinal and Aromatic Plants, P.O.–CIMAPLucknowIndia
  5. 5.Upper Assam Advisory CentreTea Research AssociationDikomIndia

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