The Role of Cell Growth-Related Gene Copy Number Variation in Autoimmune Thyroid Disease
Autoimmune thyroid disease (AITD) is a recurrent and refractory clinical endocrine disease. Some studies have shown that the incidence of AITD is not only related to iodine, a kind of environmental factor, but that susceptibility genes also play a crucial role in its pathogenesis. Since research on susceptibility genes is still underway, the aims of this study were to assess the association between copy number variations (CNVs) and AITD, to identify genes related to susceptibility to AITD, and to explore the risk factors in the occurrence of AITD. Blood samples from five AITD patients and five controls from each area were assessed by chromosome microarray to identify candidate genes. The copy number (CN) of the candidate genes and urinary iodine levels were determined in adults, including 158 AITD patients and 181 controls, from areas having different iodine statuses. The cell growth-related genes, glypican 5 (GPC5), B9 domain containing 2 (B9D2), and ankyrin repeat and suppressor of cytokine signaling [SOCS] box-containing protein family 11 (ASB11), were selected as the candidate genes. The distribution of GPC5, B9D2, and ASB11 CNVs in AITD patients and controls was significantly different, and high urinary iodine levels and GPC5 CNVs are risk factors for AITD. There was no significant association between urinary iodine level and CNVs of the candidate genes. High urinary iodine levels and GPC5 CNVs are risk factors for AITD, but an association with the occurrence of AITD was not found.
KeywordsCopy number variation GPC5 B9D2 ASB11 Autoimmune thyroid diseases Urinary iodine
We are grateful for the assistance by the Institute for Prevention and Treatment of Endemic Disease of Shanxi Province. And we also thank the colleagues who took part in the survey for their dedication and support.
The contribution of each author is as follows: Hongmei Shen and Yunfeng Guan designed the research; Yunfeng Guan, Hongmei Shen, Xing Jin, Yi Pang, Lixiang Liu, Qingzhen Jia, Fangang Meng, and Xiaoye Zhang conducted the research; Yunfeng Guan analyzed the data; Yunfeng Guan wrote the paper; Hongmei Shen had primary responsibility for final content. All the authors read and approved the final manuscript.
This work was supported by the National Natural Science Foundation of China (Grant No. 81573098).
Compliance with Ethical Standards
All the procedures used in this experiment were reviewed and approved by the biomedical research ethics committee of Harbin Medical University.
Conflict of Interest
The authors declare that they have no conflicts of interest.
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