Synergistic Effects of Selenium Nanoparticles and Vitamin E on Growth, Immune-Related Gene Expression, and Regulation of Antioxidant Status of Nile Tilapia (Oreochromis niloticus)

  • Mahmoud A. O. DawoodEmail author
  • Mohsen Zommara
  • Nabil M. Eweedah
  • Azmy I. Helal


The present study was conducted to investigate the effects of nano-selenium (Nano Se) or/and vitamin E (VE) on growth performance, blood health, intestinal histomorphology, oxidative status, and immune-related gene expression of Nile tilapia. Nano Se or/and VE at a rate of 0, 1 mg Nano Se/kg, 100 mg VE/kg, and 1 mg Nano Se/kg + 100 mg VE diet were fed to fish for 8 weeks. FBW was significantly (P < 0.05) increased in fish fed with Nano Se and VE, while fish fed with Nano Se or Nano Se and VE diets displayed significantly (P < 0.05) higher WG and SGR than the other groups. The lowest FCR was significantly (P < 0.05) detected in fish fed with Nano Se and VE, while the highest value was observed in fish VE diet. The intestinal morphometry (villi length and width) of fish fed with Nano Se or/and VE reported significantly (P < 0.05) the highest values with high number of goblet cells. Blood hematology and biochemistry parameters of fish fed with Nano Se or/and VE showed normal values with insignificant differences except for the blood total protein increased in fish fed with Nano Se or/and VE (P < 0.05). Dietary Nano Se or Nano Se and VE significantly (P < 0.05) increased the GPX, SOD, CAT, NBT, lysozyme, and phagocytosis values with decreased MDA. Liver and spleen TNF-α and IL-1β expressions were significantly (P < 0.05) upregulated in fish fed on Nano Se or Nano Se and VE. Thus, Nano Se or/and VE can be used effectively in tilapia diets for improving the growth, intestinal health, blood health, oxidative status, and immune-related gene expression.


Growth performance Histomorphology Immunity Nano-selenium Nile tilapia Oxidative status Vitamin E 


Funding Information

This work was financially supported in the framework of the project “Biological production of nano-selenium spheres and its application in livestock production” by the National Strategy for Genetic Engineering and Biotechnology, Academy of Scientific Research and Technology, Egypt.

Compliance with Ethical Standards

The experimental protocol was approved by the research animal care and use committee of the Faculty of Agriculture Kafrelsheikh University, Egypt.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Department of Animal Production, Faculty of AgricultureKafrelsheikh UniversityKafrelsheikhEgypt
  2. 2.Department of Dairy Science, Faculty of AgricultureKafrelsheikh UniversityKafrelsheikhEgypt

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