Amelioration of Cd-Induced Oxidative Stress, MT Gene Expression, and Immune Damage by Vitamin C in Grass Carp Kidney Cells

  • Yulin Yin
  • Peijun Zhang
  • Jia Liu
  • Nan Wang
  • Xinchi Shang
  • Yilin Zhang
  • Yuehong LiEmail author


Cadmium (Cd) is the most common heavy metal and is easily detected in aquatic environments on a global scale. Vitamin C was a widely used vitamin in aquaculture. The aim of this study was to explore the potential of vitamin C in ameliorating Cd-induced toxicity in grass carp kidney (CIK) cells. Cell viability, oxidative response, metallothionein (MT), and immune-related gene expression was analyzed in the present study. The results show that cell viability was significantly reduced following Cd exposure, but the vitamin C supplementation attenuated the increased in cell viability. In addition, vitamin C supplementation can increase the antioxidation response and MT and immune-related gene expression. These results indicate that vitamin C has the potential to alleviate the effects of Cd toxicity in CIK cells.


Cd Vitamin C CIK cells Oxidative response 


Funding Information

The work was supported by the National Natural Sciences Foundational of China (no. 30972191) and the 948 Program from Ministry of Agriculture of China (no. 2014Z34).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yulin Yin
    • 1
    • 2
  • Peijun Zhang
    • 3
  • Jia Liu
    • 1
    • 2
  • Nan Wang
    • 1
    • 2
  • Xinchi Shang
    • 1
    • 2
  • Yilin Zhang
    • 1
    • 2
  • Yuehong Li
    • 1
    • 2
    Email author
  1. 1.College of Animal Science and TechnologyJilin Agricultural UniversityChangchunChina
  2. 2.Ministry of education laboratory of animal production and quality securityJilin Agricultural UniversityChangchunChina
  3. 3.Health Monitoring and Inspection Center of Jilin ProvinceChangchunChina

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