Amelioration of Cd-Induced Oxidative Stress, MT Gene Expression, and Immune Damage by Vitamin C in Grass Carp Kidney Cells
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Cadmium (Cd) is the most common heavy metal and is easily detected in aquatic environments on a global scale. Vitamin C was a widely used vitamin in aquaculture. The aim of this study was to explore the potential of vitamin C in ameliorating Cd-induced toxicity in grass carp kidney (CIK) cells. Cell viability, oxidative response, metallothionein (MT), and immune-related gene expression was analyzed in the present study. The results show that cell viability was significantly reduced following Cd exposure, but the vitamin C supplementation attenuated the increased in cell viability. In addition, vitamin C supplementation can increase the antioxidation response and MT and immune-related gene expression. These results indicate that vitamin C has the potential to alleviate the effects of Cd toxicity in CIK cells.
KeywordsCd Vitamin C CIK cells Oxidative response
The work was supported by the National Natural Sciences Foundational of China (no. 30972191) and the 948 Program from Ministry of Agriculture of China (no. 2014Z34).
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Conflict of Interest
The authors declare that they have no conflict of interest.
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