Effect of Prepubertal Exposure to CdCl2 on the Liver, Hematological, and Biochemical Parameters in Female Rats; an Experimental Study

  • Saman Saedi
  • Mohammad Reza Jafarzadeh ShiraziEmail author
  • Mehdi Totonchi
  • Mohammad Javad Zamiri
  • Amin Derakhshanfar


The examination chemical factors including industrial toxins and heavy metals seem to be crucial during the prepubertal period. In order to investigate the effects of prepubertal exposure to toxic doses of Cd on liver, hematological, and biochemical parameters in the serum, 16 female rats weaned on postnatal day (PND) 21 were randomly divided into four groups with four rats in each (n = 4). The treatments were as follows: control (0.5 mL distilled water), 25, 50, and 75 mg/kg/day received cadmium chloride (CdCl2). The CdCl2 were administered orally from PND 21 days until observed first vaginal opening (VO). The result showed that the treatment of 75 mg/kg CdCl2 dramatically increased the serum level of LDL (P < 0.0001) and LDL/HDL ratio (P = 0.0004). Conversely, treatment of 75 mg/kg CdCl2 considerably decreased the serum level of HDL in comparison with control group (P = 0.0002). Nevertheless, the rats that received different doses of CdCl2 showed no significant differences in Glu, TG, and TC compared to control group. Number of RBC and Hb of rats treated with 75 mg/kg CdCl2 were significantly less than the other groups (P < 0.0001), whereas a number of WBCs in rats treated with 75 mg/kg CdCl2 (5.27 ± 0.13 103/μL) showed significant difference (P < 0.0001) compared to control group (4.23 ± 0.09 103/μL). Histopathological exams showed nodular accumulation of lymphocytes in the liver (lymphocytic hepatitis) of rats, treated with 75 mg/kg CdCl2. These results showed that CdCl2 could cause change in serum lipidome and hematological parameters. What is more, exposure to Cd triggers liver injury and cardiovascular disease during the prepubertal period.


Cadmium Liver Lipid profile Hematological parameters Lymphocytic hepatitis 



The authors are grateful to members of the Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz.

Funding Information

The Shiraz University provided financial support.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interests.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Authors and Affiliations

  1. 1.Department of Animal Science, College of AgricultureShiraz UniversityShirazIran
  2. 2.Department of GeneticsRoyan Institute for Reproductive Biomedicine, ACECRTehranIran
  3. 3.Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical SciencesShiraz University of Medical SciencesShirazIran
  4. 4.Center of Comparative and Experimental MedicineShiraz University of Medical SciencesShirazIran

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