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Effects of Dietary Iron on Manganese Utilization in Broilers Fed with Corn-Soybean Meal Diet

Abstract

To investigate the effects of dietary iron (Fe) levels on manganese (Mn) utilization, 900 8-day-old broilers were randomly assigned to 1 of 6 treatments in a 3 (Fe level) × 2 (Mn level) factorial arrangement after feeding Mn- and Fe-unsupplemented diet for 7 days. The broilers were then fed with basal corn-soybean meal diets (approximately 28 mg Mn/kg and 60 mg Fe/kg) added with 0, 80, or 160 mg/kg Fe (L-Fe, M-Fe, or H-Fe), and 0 or 100 mg/kg Mn for 35 days. Body weight gain was lower for H-Fe broilers than that for L-Fe and M-Fe broilers. On day 42, H-Fe broilers had lower serum Mn concentration as compared with L-Fe and M-Fe broilers, and tibia Mn concentration decreased as dietary Fe increased. In Mn-supplemented broilers, liver Mn was lower in L-Fe and H-Fe treatments than that in M-Fe treatment. H-Fe treatment decreased Mn concentration and manganese-containing superoxide dismutase (MnSOD) activity in the heart when compared with L-Fe and M-Fe treatments. Dietary Fe did not significantly influence Mn concentrations in the liver and heart, and heart MnSOD activity in Mn-unsupplemented broilers. In the duodenum, L-Fe treatment decreased divalent metal transporter 1 (DMT1) mRNA abundance when compared with M-Fe and H-Fe treatments, and ferroportin 1 (FPN1) mRNA level was higher in M-Fe treatment than that in L-Fe and H-Fe treatments. These results suggested H-Fe diet decreased Mn status in broilers evaluated by Mn concentrations in serum and heart, and heart MnSOD activity. Dietary Fe influenced Mn absorption possibly through effects on duodenal DMT1 and FPN1 expression.

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Acknowledgments

The authors wish to thank Dr. Greg Fraley and Ms. Brooke Van Wyk (Hope College, Holland, MI, USA) for their critical comments during the preparation of this

manuscript.

Funding

This work was supported by the National Key Research and Development Program of China (2016YFD05005504), the National Natural Science Foundation of China (31001018), Sichuan International Cooperation Project (2017HH0051), and Chinese Chelota Group Research Project (2016LD0001).

Author information

Correspondence to Shiping Bai.

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All animal care and treatment procedures used in the current study were approved by the Animal Care and Use Committee of Sichuan Agricultural University.

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The authors declare that they have no conflict of interest.

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Bai, S., Peng, J., Zhang, K. et al. Effects of Dietary Iron on Manganese Utilization in Broilers Fed with Corn-Soybean Meal Diet. Biol Trace Elem Res 194, 514–524 (2020). https://doi.org/10.1007/s12011-019-01780-w

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Keywords

  • Iron
  • Manganese
  • Manganese-containing superoxide dismutase
  • Ferroportin 1
  • Broiler