Potential Role of Selenium Against Hepatotoxicity Induced by 2,4-Dichlorophenoxyacetic Acid in Albino Wistar Rats

  • Lazhari Tichati
  • Fouzia Trea
  • Kheireddine OualiEmail author


The present study aims to investigate the hepatoprotective effects of selenium on toxicity induced by ‘Désormone Lourd’ based on 2,4-dichlorophenoxyacetic acid in Wistar rats. Male Wistar rats were divided into four groups and were treated orally. The (C) group was used as a control, while the test groups were treated with Se (0.2 mg/kg b.w.), 2,4-D (5 mg/kg b.w.) or both (2,4-D + Se) for 4 weeks. Our results showed that chronic treatment with 2,4-D resulted in hepatotoxicity, as revealed by an increase in liver function markers Aminotransferases (ALT, AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total bilirubin (TB), along with reduced total protein content and albumin. An overall pro-oxidant effect was associated with a decrease in the reduced glutathione (GSH) content and the enzymatic activity of glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), and an increase in malondialdehyde (MDA) and protein carbonyl levels (PCO). Microscopic observation of liver in 2,4-D-treated rats reveals lesions, which results in perivascular inflammatory infiltration around the vessel, sinusoidal dilatation and vacuolization of hepatocytes. However, selenium supplementation in 2,4-D-treated rats elicited a reduction in the toxic effects of the pesticide by improving the studied parameters, which was confirmed by the histological study of the liver. Selenium appears to have a promising prophylactic effect through its effective anti-radical action against the hepatotoxic effects of 2,4-D.


2,4-D Antioxidant enzymes Hepatotoxicity Oxidative stress Rat Selenium 


Funding Information

This research is supported by the General Direction of Scientific Research and Development of Technology and Ministry of Higher Education and Scientific Research, DGRSDT-MESRS Algeria.

Compliance with Ethical Standards

All experimental procedures were conducted according to the International Guidelines for Laboratory Animal Care and Use (Council of European Communities) [39] and were approved by the University Ethics Committee.


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Authors and Affiliations

  1. 1.Laboratory of Environmental Biosurveillance (LBSE), Department of Biology, Faculty of SciencesBadji Mokhtar UniversityAnnabaAlgeria

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