Status of Serum Copper, Magnesium, and Total Antioxidant Capacity in Patients with Polycystic Ovary Syndrome
This study evaluates serum copper and magnesium and total antioxidant capacity levels in PCOS patients. In this regard, the probable association of copper and magnesium with total antioxidant capacity (TAC) was investigated. In total, 150 women (60 PCOS patients and 90 healthy subjects) participated in this case–control study. PCOS was diagnosed according to the Rotterdam criteria (2003). Serum Cu, Mg, Ca, TAC, insulin levels, and insulin resistance indices were determined. Insulin was measured using ELISA methods. Serum Cu and Mg levels were measured by an atomic absorption spectrophotometer and the Xylidyl Blue method respectively. The correlations between the parameters were analyzed using the Spearman correlation test. Serum Cu level was significantly higher while TAC was significantly lower in the PCOS patients than those in the controls (p = 0.019 and p = 0.002 respectively). No significant difference was detected between the two groups in terms of serum Mg and Ca levels and Ca/Mg ratio. In insulin-resistant PCOS subjects, there was a negative correlation between Mg levels and homeostatic model assessment for insulin resistance (r = − 0.449, p = 0.006) but a positive correlation between Mg levels and quantitative insulin sensitivity check index (r = 0.480, p = 0.003). A negative correlation also existed between Mg levels and TAC in non-insulin-resistant PCOS patients (r = − 0.407, p = 0.04). According to the results, copper and magnesium seem to contribute to oxidative stress and insulin resistance in PCOS patients. Therefore, to prevent long-term metabolic complications in PCOS women, it is recommended that these elements be routinely monitored. Also, significantly lower levels of serum TAC in PCOS patients than in normal women may suggest increased oxidative stress in such patients.
KeywordsPolycystic ovary syndrome Insulin resistance Copper Magnesium Total antioxidant capacity
Polycystic ovary syndrome
Total antioxidant capacity
- Ca/Mg ratio
- G/I ratio
Body mass index
Fasting blood sugar
Homeostatic model assessment for insulin resistance
Quantitative insulin sensitivity check index
We would like to thank the participating patients as well as the staff of Fatemeh Zahra Infertility and Reproductive Health Research Center for their valuable cooperation with this project.
We are grateful for the financial support granted by Babol University of Medical Sciences, Iran.
Compliance with Ethical Standards
Approval was obtained from the ethics committee of Babol University of Medical Sciences (MUBABOL.REC.1394.65). All the participants were informed of the aims of the study, and informed consents were obtained from them. The biochemical tests had no cost for the participants, and the results of these tests were reported to them.
Conflict of Interest
The authors declare that they have no conflict of interest.
- 2.Rahsepar M, Mahjoub S, Esmaeilzadeh S, Kanafchian M, Ghasemi M (2017) Evaluation of vitamin D status and its correlation with oxidative stress markers in women with polycystic ovary syndrome. Int J Reprod Biomed 15:345–350Google Scholar
- 7.Kanafchian M, Mahjoub S, Esmaeilzadeh S, Rahsepar M, Mosapour A (2018) Status of serum selenium and zinc in patients with the polycystic ovary syndrome with and without insulin resistance. Middle East Fertil Soc J 23:241–245Google Scholar
- 11.Swetha N, Vyshnavi R, Modagan P, Rajagopalan B (2013) A correlative study of biochemical parameters in polycystic ovarian syndrome. Int J Biol Med Res 4:3148–3154Google Scholar
- 12.Maleedhu P, Vijayabhaskar M, Rao P, Kodumuri P (2014) Antioxidant status in women with polycystic ovary syndrome. Int J Med Health Sci 3:91–96Google Scholar
- 14.Azziz R (2006) Diagnosis of polycystic ovarian syndrome: the Rotterdam criteria arepremature. J Clin Endocrinol Metab 91:781–785Google Scholar
- 19.Chakraborty P, Ghosh S, Goswami S, Kabir SN, Chakravarty B, Jana K (2013) Altered trace mineral milieu might play an aetiological role in the pathogenesis of polycystic ovary syndrome. Biol Trace Elem Res 152:9–15Google Scholar
- 22.Kurdoglu Z, Ozkol H, Tuluce Y, Koyuncu I (2012) Oxidative status and its relation with insulin resistance in young nonobese women with polycystic ovary syndrome. J Endocrinol Investig 35:317–321Google Scholar
- 25.Mahjoub S, Masrour-Roudsari J (2012) Role of oxidative stress in pathogenesis of metabolic syndrome. Caspian J Intern Med 3:386–396Google Scholar
- 26.Rajeswari G, Gopal PS, Veerabhadrudu B, Suresh E (2016) Study of magnesium levels in polycystic ovarian syndrome. Int J Adv Sci Res 2:054–058Google Scholar
- 27.Usharani M, Santhanalakshmi L (2015) Study of magnesium level in polycystic ovarian syndrome. Int J Adv Res 3:1456–1461Google Scholar
- 31.Moti M, Amini L, Mirhoseini Ardakani SS, Kamalzadeh S, Masoomikarimi M, Jafarisani M (2015) Oxidative stress and anti-oxidant defense system in Iranian women with polycystic ovary syndrome. Iran J Reprod Med 13:373–378Google Scholar
- 32.Kandasamy S, Inmozhi Sivagamasundari R, Bupathy A, Sethubathy S, Gobal V (2010) Evaluation of insulin resistance and oxidative stress in obese patients with polycystic ovary syndrome. Int J Appl Biol Pharm Technol 1:391–398Google Scholar