Advertisement

Effects of Fungal Polysaccharide on Oxidative Damage and TLR4 Pathway to the Central Immune Organs in Cadmium Intoxication in Chickens

  • Ruyue Li
  • Linan Zhang
  • Zequn Tang
  • Tianqi Li
  • Guangxing Li
  • Ruili ZhangEmail author
  • Ming GeEmail author
Article
  • 31 Downloads

Abstract

Cadmium (Cd) can cause animal organism damage, but there have been few studies on the damage of cadmium to the immune organs of birds. Most fungal polysaccharide has antioxidant and immunomodulatory effects. The experimental study investigated the effects of fungal polysaccharide (Agaricus blazei Murill polysaccharide and Ganoderma luciduccharide) on the oxidative damage of central immune organs (thymus and bursa of Fabricius) and on the Toll-like receptor 4 (TLR4) pathway in cadmium-poisoned chickens. The results showed that Agaricus blazei polysaccharide and Ganoderma lucidum polysaccharide can reduce cadmium content, TLR4 expression, inflammatory factor (IL-1β, IL-6, TNF-α) content, and lipid peroxidation product MDA content and increase the activity of antioxidant enzymes SOD and GSH-Px in thymus and bursa of cadmium poisoning chickens. Ganoderma lucidum polysaccharide could decrease the expression of TLR4, IL-1β, and IL-6 in cadmium poisoning peripheral blood lymphocytes of chicken, and TLR4 inhibitor had the same effect. The results demonstrated the protective effects of Agaricus blazei Murill polysaccharide and Ganoderma lucidum polysaccharides on the damage of the central immune organs of chickens caused by cadmium poisoning were closely related to the TLR4 signaling pathway and oxidative stress.

Keywords

Cadmium Fungal polysaccharide Chicken TLR4 signaling pathway Antioxidants Immune organs 

Notes

Acknowledgements

We thank the members of the Traditional Chinese Veterinary Medicine Laboratory at the College of Veterinary Medicine, Northeast Agricultural University.

Funding Information

This work was supported by the National Science Foundation of China (Grant No. 31272533).

Compliance with Ethical Standards

Ethical treatment of animals used in this study was approved by the Animal Welfare Committee protocol (#NEAU-2013-02-0252-11) at Northeast Agricultural University (Harbin, China).

Conflict of Interest

The authors declare that they have no conflict of interest.

Disclaimer

All authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the journal.

References

  1. 1.
    Burger J (2008) Assessment and management of risk to wildlife from cadmium. Sci Total Environ 389(1):37–45CrossRefGoogle Scholar
  2. 2.
    Patar A, Giri A, Boro F, Bhuyan K, Singha U, Giri S (2016) Cadmium pollution and amphibians - studies in tadpoles of Rana limnocharis. Chemosphere 144:1043–1049CrossRefGoogle Scholar
  3. 3.
    Huo J, Dong A, Yan J, Wang L, Ma C, Lee S (2017) Cadmium toxicokinetics in the freshwater turtle, Chinemys reevesii. Chemosphere 182:392–398CrossRefGoogle Scholar
  4. 4.
    Brzóska MM, Majewska K, Moniuszkojakoniuk J (2004) Mineral status and mechanical properties of lumbar spine of female rats chronically exposed to various levels of cadmium. Bone 34(3):517–526CrossRefGoogle Scholar
  5. 5.
    Marth E, Barth S, Jelovcan S (2000) Influence of cadmium on the immune system. Description of stimulating reactions. Cent Eur J Public Health 8(1):40–44PubMedGoogle Scholar
  6. 6.
    Calabrese V, Cighetti R, Peri F (2015) Molecular simplification of lipid a structure: TLR4-modulating cationic and anionic amphiphiles. Mol Immunol 63(2):153–161CrossRefGoogle Scholar
  7. 7.
    Pascuallucas M, Fernandezlizarbe S, Montesinos J, Guerri C (2014) LPS or ethanol triggers clathrin- and rafts/caveolae-dependent endocytosis of TLR4 in cortical astrocytes. J Neurochem 129(3):448–462CrossRefGoogle Scholar
  8. 8.
    Oblak A, Pohar J, Jerala R (2015) MD-2 determinants of nickel and cobalt-mediated activation of human TLR4. 10(3):e0120583Google Scholar
  9. 9.
    Song SY, Bae CH, Choi YS, Kim YD (2016) Cadmium induces mucin 8 expression via Toll-like receptor 4-mediated extracellular signal related kinase 1/2 and p38 mitogen-activated protein kinase in human airway epithelial cells. Int Forum Allergy Rhinol 6(6):638–645CrossRefGoogle Scholar
  10. 10.
    Oshiman K, Fujimiya Y, Ebina T, Suzuki I, Noji M (2002) Orally administered beta-1,6-D-polyglucose extracted from Agaricus blazei results in tumor regression in tumor-bearing mice. Planta Med 68(07):610–614CrossRefGoogle Scholar
  11. 11.
    Liu Z, Ma X, Deng B, Huang Y, Bo R, Gao Z, Yu Y, Hu Y, Liu J, Wu Y (2015) Development of liposomal Ganoderma lucidum polysaccharide: formulation optimization and evaluation of its immunological activity. Carbohydr Polym 117:510–517CrossRefGoogle Scholar
  12. 12.
    Shi M, Zhang Z, Yang Y (2013) Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP). Carbohydr Polym 95(1):200–206CrossRefGoogle Scholar
  13. 13.
    Song Y, Zhang R, Wang H, Yan Y, Ming G (2017) Protective effect of Agaricus blazei polysaccharide against cadmium-induced damage on the testis of chicken. Biol Trace Elem Res:1–10Google Scholar
  14. 14.
    Hu X, Zhang R, Xie Y, Wang H, Ge M (2016) The protective effects of polysaccharides from Agaricus blazei Murill against cadmium-induced oxidant stress and inflammatory damage in chicken livers. Biol Trace Elem Res 178(1):1–10Google Scholar
  15. 15.
    Daley T, Omoregie SN, Wright V, Omoruyi FO (2013) Effects of phytic acid and exercise on some serum analytes in rats orally exposed to diets supplemented with cadmium. Biol Trace Elem Res 151(3):400–405CrossRefGoogle Scholar
  16. 16.
    Satarug S, Garrett SH, Sens MA, Sens DA (2011) Cadmium, environmental exposure, and health outcomes. Environ Health Perspect 16(5):182–190Google Scholar
  17. 17.
    Xie W, Lv A, Li R, Tang Z, Ma D, Huang X, Zhang R, Ge M (2017) Agaricus blazei Murill polysaccharides protect against cadmium-induced oxidative stress and inflammatory damage in chicken spleens. Biol Trace Elem Res (1):1–12Google Scholar
  18. 18.
    Zhang R, Yu Q, Shi G, Rui L, Zhang W, Xia Z, Li G, Ming G (2017) chTLR4 pathway activation by Astragalus polysaccharide in bursa of Fabricius. BMC Vet Res 13(1):119CrossRefGoogle Scholar
  19. 19.
    Shagirtha K, ., Muthumani M, ., S M Prabu (2011) Melatonin abrogates cadmium induced oxidative stress related neurotoxicity in rats. Eur Rev Med Pharmacol Sci 15 (9):1039, 1050PubMedGoogle Scholar
  20. 20.
    Zhao P, Guo Y, Zhang W, Chai H, Xing H, Xing M (2017) Neurotoxicity induced by arsenic in Gallus Gallus: regulation of oxidative stress and heat shock protein response. Chemosphere 166:238–245CrossRefGoogle Scholar
  21. 21.
    Jia S, Li F, Liu Y, Ren H, Gong G, Wang Y, Wu S (2013) Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill. Int J Biol Macromol 62(11):66–69CrossRefGoogle Scholar
  22. 22.
    Pan K, Jiang Q, Liu G, Miao X, Zhong D (2013) Optimization extraction of Ganoderma lucidum polysaccharides and its immunity and antioxidant activities. Int J Biol Macromol 55(2):301–306CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Veterinary MedicineNortheast Agricultural UniversityHarbinChina
  2. 2.Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and TreatmentNortheast Agricultural UniversityHarbinChina

Personalised recommendations