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Nutraceutical Effect of Trace Elements as Additional Injectable Doses to Modulate Oxidant and Antioxidant Status, and Improves the Quality of Lamb Meat

  • Chrystian J. Cazarotto
  • Jhonatan P. Boito
  • Patrícia Glombowsky
  • Rafael A. Baggio
  • Gabriela M. Galli
  • Gustavo Machado
  • Nathieli B. Bottari
  • Marta L. R. Leal
  • Julcemar D. Kessler
  • Matheus D. Baldissera
  • Aleksandro S. da SilvaEmail author
Article
  • 13 Downloads

Abstract

Our study aimed to evaluate whether zinc, copper, selenium, and manganese subcutaneous mineral application (trace elements) reduced mortality, improved performance, and modulated oxidant and antioxidant balance in lamb meat, thereby improving its quality. We divided the 110 newborn Lacaune lambs into two groups: non-treated (control), and treated (application of minerals) with three doses of 0.33 mL/kg of body weight mineral complex on days of life 1, 30, and 60. All animals were weighed on day of life 1, 30, 60, 90, and 150. At the end of the experiment, 12 animals were slaughtered for physical and chemical analysis of meat, oxidant, and antioxidant status, and for allometric analysis. Mineral-application animals had greater live-weight (P < 0.05) on days of life 60 and 90. There was an increase in fat thickness (P = 0.004); pH levels (P = 0.002) were lower in mineral-application animal meat than in that of the control group. Meat was paler (according to lightness (L color)) in the control group (P = 0.04). Weight loss from cooking was greater in control animals (P = 0.004). Shear strength values were lower in the meat of treated lambs (P = 0.008) suggesting that mineral application was associated with increased meat tenderness. In addition, catalase and superoxide dismutase activities were higher (P = 0.01) in mineral-treated animals, associated with a reduction in reactive oxygen species levels (P < 0.01), and lipid peroxidation products (P = 0.02). These data suggest that mineral application modulated oxidant and antioxidant status, reflecting better meat quality.

Keywords

Performance Lipid oxidation Reactive oxygen species Antioxidant system Mineral 

Notes

Acknowledgments

We thank CAPES, CNPq, and UDESC (PROMOP) for the scholarship and research.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethics Committee

This study was approved by the Ethics Committee of Use of Animals (CEUA) of Universidade do Estado de Santa Catarina (UDESC), protocol number 7398301116, as well as with the rules issued by the National Council for Control of Animal Experimentation (CONCEA).

References

  1. 1.
    Andrade LS, Marreiro DN (2011) Aspectos sobre a relação entre exercício físico, estresse oxidativo e zinco. Rev Nutr 24:4–10CrossRefGoogle Scholar
  2. 2.
    Asad NR, Asad LMBO, Almeida CEBD, Felzenszwalb I, Cabral-Neto JB, Leitão AC (2004) Several pathways of hydrogen peroxide action that damage the E. coli genome. Genet Mol Biol 27:291–303CrossRefGoogle Scholar
  3. 3.
    Baldissera MD, Souza CF, Grando TH, Dolci GS, Cossetin LF, Moreira KLS, Da Veiga ML, Da Rocha MIUM, Boligon AA, De Campos MMA, Stefani LM, Da Silva AS, Monteiro SG (2017) Nerolidol-loaded nanospheres prevent hepatic oxidative stress of mice infected by Trypanosoma evansi. Parasitology 144:148–157CrossRefGoogle Scholar
  4. 4.
    Barreiros ALBS, David JM, David JP (2006) Oxidative stress: relative between the formation of reactive species and the organism’s defense. Quím Nova 29:113–123CrossRefGoogle Scholar
  5. 5.
    Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76CrossRefGoogle Scholar
  6. 6.
    Boleman SJ, Boleman SL, Miller RK, Taylor JF, Cross HR, Wheeler TL, Koohmaraie M, Shackelford SD, Miller MF, West RL, Johnson DD, Savell JW (1997) Consumer evaluation of beef of known categories of tenderness. J Anim Sci 75:1521–1524CrossRefGoogle Scholar
  7. 7.
    Bray AR, Graafhuis AE, Chrystall BB (1989) The cumulative effect of nutritional, shearing and pre slaughter washing stresses on the quality of lamb meat. Meat Sci 25:59–67CrossRefGoogle Scholar
  8. 8.
    Brodin O, Eksborg S, Wallenberg M, Asker-Hagelberg C, Larsen EH, Mohlkert D, Lenneby-Helleday C, Jacobsson H, Linder S, Misra S, Bjornstedt M (2015) Pharmacokinetics and toxicity of sodium selenite in the treatment of patients with carcinoma in a phase I clinical trial: the SECAR study. Nutrients 7:4978–4994CrossRefGoogle Scholar
  9. 9.
    Cazarotto CJ, Boito JP, Gebert RR, Reis JH, Machado G, Bottari NB, Morsch VM, Schetinger MRC, Doleski PH, Leal MLR, Baldissera MD, Da Silva AS (2018) Metaphylactic effect of minerals on immunological and antioxidant responses, weight gain and minimization of coccidiosis of newborn lambs. Res Vet Sci 121:46–52CrossRefGoogle Scholar
  10. 10.
    Colpo E, de Bem AF, Pieniz S, Schettert SD, dos Santos RM, Farias IL, Bertoncello I, Moreira CM, Barbosa NV, Moretto MB, Rocha JB (2008) A single high dose of ascorbic acid and iron is not correlated with oxidative stress in healthy volunteers. Ann Nutr Metab 53:79–85CrossRefGoogle Scholar
  11. 11.
    Contassot E, Beer HD, French LE (2012) Interleukin-1, inflammasomes, autoinflammation and the skin. Swiss Med Wkly 142:w13590PubMedGoogle Scholar
  12. 12.
    Devine CE, Graafhuis AE, Muir PD, Chrystall BB (1993) The effect of growth rate and ultimate pH on meat quality of lambs. Meat Sci 35:63–77CrossRefGoogle Scholar
  13. 13.
    Do Carmo TJ, Peripolli V, Costa JBG, Tanure CB, Fioravanti MCS, Restle J, Kindlein L, McManus C (2017) Carcass characteristics and meat evaluation of Nelore cattle subjected to different antioxidant treatments. Rev Bras Zootec 46:138–146CrossRefGoogle Scholar
  14. 14.
    Farinati F, Cardin R, D’Inca R, Naccarato R, Sturniolo GC (2003) Zinc treatment prevents lipid peroxidation and increases glutathione availability in Wilson’s disease. J Lab Clin Med 141:372–377CrossRefGoogle Scholar
  15. 15.
    Flores EMM, Saidelles APF, Barin JS, Mortari SR, Martins AF (2001) Hair sample decomposition using polypropylene vials for determination of arsenic by hydride generation atomic absorption spectrometry. J Anal At Spectrom 16:1419–1423CrossRefGoogle Scholar
  16. 16.
    Garcia A, Purchas RW, Kadim IT, Yamamoto SM (2005) Meat quality in lambs of different genotypes and ages at slaughter. Rev Bras Zootec 34:1070–1078CrossRefGoogle Scholar
  17. 17.
    Girotti AW, Thomas JP, Jordan JE (1985) Inhibitory effect of zinc (II) on free radical lipid peroxidation in erythrocyte membranes. J Free Radic Biol Med 1:395–401CrossRefGoogle Scholar
  18. 18.
    Hall JA, Vorachek WR, Stewart CW, Gorman ME, Mosher WD, Pirelli GJ, Bobe G (2013) Selenium supplementation restores innate and humoral immune responses in footrot-affected sheep. PlosOne 8:e82572CrossRefGoogle Scholar
  19. 19.
    Halliwell B, Gutteridge JMC (2007) Free radicals in biology and medicine. 4. Clarendon, OxfordGoogle Scholar
  20. 20.
    Halliwell B, Whiteman M (2004) Measuring reactive oxygen species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 142:231–255CrossRefGoogle Scholar
  21. 21.
    Holen JP, Rambo Z, Hilbrands AM, Johnston LJ (2018) Effects of dietary zinc source and concentration on performance of growing-finishing pigs reared with reduced floor space. Prof Anim Sci 34:133–143CrossRefGoogle Scholar
  22. 22.
    Kassahn KS, Crozier RH, Portner HO, Caley MJ (2009) Animal performance and stress: responses and tolerance limits at different levels of biological organization. Biol Rev Camb Philos Soc 84:277–292CrossRefGoogle Scholar
  23. 23.
    Khan AZ, Kumbhar S, Liu Y, Hamid M, Pan C, Nido SA, Parveen F, Huang K (2018) Dietary supplementation of selenium-enriched probiotics enhances meat quality of broiler chickens (Gallus gallus domesticus) raised under high ambient temperature. Biol Trace Elem Res 182:328–338CrossRefGoogle Scholar
  24. 24.
    Kralik G, Kralik Z (2017) Poultry products enriched with nutricines have beneficial effects on human health. Med Glas 14:1–7Google Scholar
  25. 25.
    Martin LCT (1993) Nutrição mineral de bovinos de corte. Nobel, São Paulo, p 173Google Scholar
  26. 26.
    McCord JM, Fridovich I (1969) Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049–6055PubMedGoogle Scholar
  27. 27.
    Morris ST (2009) Economics of sheep production. Small Rumin Res 86:59–62CrossRefGoogle Scholar
  28. 28.
    Murphy MP, Holmgren A, Larsson NG, Halliwell B, Chang CJ, Kalyanaraman B, Rhee SG, Thornalley PJ, Partridge L, Gems D, Nystrom T, Belousov V, Schumacker PT, Winterbourne CC (2011) Unraveling the biological roles of reactive oxygen species. Cell Metab 13:361–366CrossRefGoogle Scholar
  29. 29.
    Nelson DP, Kiesow LA (1972) Enthalpy of decomposition of hydrogen peroxide by catalase at 25°C (with molar extinction coefficients of H2O2 solutions in the UV). Anal Biochem 49:474–478CrossRefGoogle Scholar
  30. 30.
    NRC – National Research Council (2007) Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids. The National Academies Press, Washington, DC.  https://doi.org/10.17226/11654 CrossRefGoogle Scholar
  31. 31.
    Ohkawa H, Ohishi N, Yagi K (1978) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefGoogle Scholar
  32. 32.
    Paz Matias J, Costa e Silva DM, Clímaco Cruz KJ, Gomes da Silva K, Oliveira Medeiros LG, do Nascimento Marreiro D, do Nascimento Nogueira N (2014) Effect of zinc supplementation on superoxide dismutase activity in patients with ulcerative rectolitis. Nutr Hosp 31:1434–1437PubMedGoogle Scholar
  33. 33.
    Pethick DW, Ball AJ, Banks RG, Hocquette JF (2011) Current and future issues facing red meat quality in a competitive market and how to manage continuous improvement. Anim Prod Sci 51:13–18CrossRefGoogle Scholar
  34. 34.
    Salim HM, Lee H, Jo C, Lee SK, Lee BD (2011) Supplementation of graded levels of organic zinc in the diets of female broilers: effects on performance and carcase quality. Br Poult Sci 52:606–612CrossRefGoogle Scholar
  35. 35.
    Schneider CD, Oliveira AR (2004) Radicais livres de oxigênio e exercício: mecanismos de formação e adaptação ao treinamento físico. Rev Bras Med Esporte 10:308–313CrossRefGoogle Scholar
  36. 36.
    Seideman SC, Cross HR, Smith GC, Durland PR (1984) Factors associated with fresh meat color: a review discusses the various color forms of myoglobin in muscle, species, atmospheric conditions, antemortem factors and storage characteristics. J Food Qual 6:211–237CrossRefGoogle Scholar
  37. 37.
    Shankar V (2015) Field characterization by near infrared (NIR) mineral identifiers—a new prospecting approach. Procedia Earth Planet Sci 11:198–203CrossRefGoogle Scholar
  38. 38.
    Shenk JS, Westerhaus MO (1994) The aplication of Near infrared reflectance spectroscopy (NIRS) to forage analysis. In: Fahey GC Jr (ed) Forage quality evaluation and utilization. American Society of Agronomy, Madison, pp 406–449Google Scholar
  39. 39.
    Silva Sobrinho AG, Silva AMA (2000) Produção de carne ovina. Rev Nac Carne 285:32–44Google Scholar
  40. 40.
    Silva MA, Santin T, Maturana FM, Lemes KM, Gonçalves RL, Mattioli GA, Mendes Lollato JP, Persico JM, Celeghini ECC, Madureira EH (2016) Effect of mineral supplementation and vitamin injection (Kit Adaptador® MIN and Adaptador® VIT, Biogenesis Bagó), associated with vaccination against reproductive diseases (Bioleptogen® and Bioabortogen® H, Biogenesis Bagó) on pregnancy rates in beef cows. Anim Reprod 13:631Google Scholar
  41. 41.
    Simões JA, Ricardo R (2000) Avaliação da cor da carne tomando como referência o músculo rectus abdominis, em carcaças de cordeiros leves. Revista Portuguesa de Ciências Veterinárias 95:124–127Google Scholar
  42. 42.
    Siqueira ER, Simões CD, Fernandes S (2001) Efeito do sexo e do peso ao abate sobre a produção de carne de cordeiros. Morfometria da carcaça, peso dos cortes, composição tecidual e componentes não constituintes da carcaça. Rev Bras Zootec 30:1299–1307CrossRefGoogle Scholar
  43. 43.
    Smyth MJ, Johnstone RW (2000) Role of TNF in lymphocyte-mediated cytotoxicity. Microsc Res Tech 50:196–208CrossRefGoogle Scholar
  44. 44.
    Soldá NM, Glombowsky P, Campigotto G, Bottari NB, Schetinger MRC, Morsch VM, Favero JF, Baldissera MD, Schogor ALB, Barreta D, Machado G, da Silva AS (2017) Injectable mineral supplementation to transition period dairy cows and its effects on animal health. Comp Clin Pathol 26:335–342CrossRefGoogle Scholar
  45. 45.
    Suttle NF (2010) Mineral nutrition of livestock, 4th edn. CABI Publishing, New YorkCrossRefGoogle Scholar
  46. 46.
    Thompson JM, Hopkins DL, D’Souza DND, Walker PJ, Baud SR, Pethick DW (2005) The impact of processing on sensory and objective measurements of sheep meat eating quality. Aust J Exp Agric 45:561–573CrossRefGoogle Scholar
  47. 47.
    Truscott TG, Hudson JE, Anderson SK (1984) Differences between observers in assessment of meat colour. Proc Aust Soc Anim Prod 15:762–764Google Scholar
  48. 48.
    Underwood EJ, Suttle NF (1999) The mineral nutrition of livestock, 3rd edn. CABI Publishing, New YorkCrossRefGoogle Scholar
  49. 49.
    Wang R, Pan X, Peng Z (2009) Effects of heat exposure on muscle oxidation and protein functionalities of pectoralis majors in broilers. Poult Sci 88:1078–1084CrossRefGoogle Scholar
  50. 50.
    Xu X, Liu L, Long SF, Piao XS, Ward TL, Ji F (2017) Effects of chromium methionine supplementation with different sources of zinc and growth performance, carcass traits, meat quality, serum metabolites, endocrine parameters, and the antioxidant status in growing-finishing pigs. Biol Trace Elem Res 179:70–78CrossRefGoogle Scholar
  51. 51.
    Yamamoto SM, Garcia A, Silvio R, Pinheiro B, Leão AG (2013) Inclusion of sunflower seeds in the diet of lambs on carcass quantitative characteristics and meat quality. Semina: Ciências Agrarias 34:1925–1934Google Scholar
  52. 52.
    Yeo JE, Kang SK (2007) Selenium effectively inhibits ROS-mediated apoptotic neural precursor cell death in vitro and in vivo in traumatic brain injury. Biochim Biophys Acta 1772:1199–1210CrossRefGoogle Scholar
  53. 53.
    Zakaria HA, Jalal M, Al-Titi HH, Souad A (2017) Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers. Revista Brasileira de Ciência Avícola 19:519–526CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chrystian J. Cazarotto
    • 1
  • Jhonatan P. Boito
    • 1
  • Patrícia Glombowsky
    • 1
  • Rafael A. Baggio
    • 1
  • Gabriela M. Galli
    • 1
  • Gustavo Machado
    • 2
  • Nathieli B. Bottari
    • 3
  • Marta L. R. Leal
    • 4
  • Julcemar D. Kessler
    • 1
  • Matheus D. Baldissera
    • 5
  • Aleksandro S. da Silva
    • 1
    • 3
    • 6
    Email author
  1. 1.Graduate Program in Animal ScienceUniversidade do Estado de Santa Catarina (UDESC)ChapecóBrazil
  2. 2.Department of Population Health and Pathobiology, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  3. 3.Graduate Program in Biochemical ToxicologicologyUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  4. 4.Department of Large AnimalUFSMSanta MariaBrazil
  5. 5.Department of Microbiology and ParasitologyUFSMSanta MariaBrazil
  6. 6.Department of Animal ScienceUniversity of Santa Catarina StateChapecóBrazil

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