Effect of Long-term Administration of Oral Magnesium Sulfate and Insulin to Reduce Streptozotocin-Induced Hyperglycemia in Rats: the Role of Akt2 and IRS1 Gene Expressions

  • Mitra Kamran
  • Fatemeh Kharazmi
  • Kianoosh Malekzadeh
  • Ardeshir Talebi
  • Fatemah Khosravi
  • Nepton SoltaniEmail author


The effects of long-term oral administration of magnesium sulfate and insulin on hyperglycemia were investigated using Akt2 and IRS1 gene expression methods in streptozotocin-induced diabetic rats. Fifty rats were randomly divided into five experimental groups: 1, non-diabetic control (NDC); 2, Mg2+-treated non-diabetic control (Mg-NDC); 3, chronic diabetic (CD); 4, Mg2+-treated chronic diabetic (Mg-CD); and 5, insulin-treated chronic diabetic (Ins-CD). Streptozotocin was used to induce diabetes. The Mg-CD and Mg-NDC groups received 10 g/l of MgSO4 added to drinking water. The Ins-CD group received 2.5 U/kg of insulin twice a day. Blood glucose level and body weight were measured every week. The intraperitoneal glucose tolerance test (IPGTT) was performed after 16 weeks. MgSO4 administration improved the blood glucose level and IPGTT. It also increased Akt2 and IRS1 genes as well as protein expression. Insulin lowered the blood glucose level and increased IRS1 gene and protein expression, but did not affect Akt2 gene and protein expression. Glucose reduction after Mg therapy may be mediated, at least partially, via IRS1 and Akt2 genes and protein stimulation. In insulin-treated rats, insulin resistance was not significant due to the absence of Akt2 gene expression.


Diabetes Magnesium Insulin Akt2 IRS1 



This study was supported by the Hormozgan University of Medical Science under grant number 9471.

Compliance with Ethical Standards

The animals were managed in agreement with the criteria defined in the NIH publication no. 85-23, revised in 1985, and the experimental protocol was approved by the Ethics Committee HUMS REC.1394.112 for Animal Care of Hormozgan University of Medical Sciences.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Physiology Department, Faculty of MedicineHormozgan University of Medical ScienceBandar AbbasIran
  2. 2.Endocrinology and Metabolism Research CenterHormozgan University of Medical SciencesBandar AbbasIran
  3. 3.Molecular Medicine Research CenterHormozgan University of Medical ScienceBandar AbbasIran
  4. 4.Clinical Pathology Department, Faculty of MedicineIsfahan University of Medical ScienceIsfahanIran
  5. 5.Physiology Department, School of MedicineIsfahan University of Medical ScienceIsfahanIran

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