Enhancement in Iron Absorption on Intake of Chemometrically Optimized Ratio of Probiotic Strain Lactobacillus plantarum 299v with Iron Supplement Pearl Millet

  • Shanta Kumari AdikiEmail author
  • Chandra Kiran Perla
  • Gargi Saha
  • Prakash Katakam
  • Vinaykumar Theendra


This research article aims to establish the intake ratio of probiotic Lactobacillus plantarum 299v with iron supplement pearl millet by central composite design of response surface methodology so as to enhance iron absorption. In anemic rat models, the food intake pattern, body weight, hemoglobin content, and hematocrit values were found to be significantly increased on treatment with pearl millet:probiotic; however, incorporation of probiotics at lower dose (0.5 g) was significantly (p < 0.05) effective in enhancing iron absorption, and further increment in probiotic doses (1.0 g) did not produce significant increase in hemoglobin and hematocrit values as evidenced by the experimental findings.


Probiotic Iron absorption Pearl millet Anemia Central composite design 



We thank the Nirmala College of Pharmacy, Mangalagiri, Guntur, Andhra Pradesh, India, for providing research facilities.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Nirmala College of PharmacyGunturIndia
  2. 2.Research Officer, National Tea Research FoundationKolkataIndia
  3. 3.Indira College of PharmacyNandedIndia

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