Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli
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Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.
KeywordsAcidithiobacillus ferrooxidans Metal resistance Expression Bioinformatics afe_1862
The authors would like to give thanks to the participants, coordinators, and administrators for their supports during the study. This work was supported by Chinese National Natural Science Foundation (No. 31460032, 31760028 and 81660581).
Compliance with Ethical Standards
Conflict of Interest
All authors declare that they have no conflicts of interest.
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