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Biological Trace Element Research

, Volume 189, Issue 1, pp 291–300 | Cite as

Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli

  • Feifan LengEmail author
  • Yuanli Li
  • Wen Luo
  • Qingwei Wei
  • Yanjun Jing
  • Xiaoli Wang
  • Mingjun Yang
  • Yonggang WangEmail author
Article
  • 127 Downloads

Abstract

Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.

Keywords

Acidithiobacillus ferrooxidans Metal resistance Expression Bioinformatics afe_1862 

Notes

Acknowledgments

The authors would like to give thanks to the participants, coordinators, and administrators for their supports during the study. This work was supported by Chinese National Natural Science Foundation (No. 31460032, 31760028 and 81660581).

Compliance with Ethical Standards

Conflict of Interest

All authors declare that they have no conflicts of interest.

Supplementary material

12011_2018_1462_MOESM1_ESM.docx (2.6 mb)
ESM 1 (DOCX 2620 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Life Science and EngineeringLanzhou University of TechnologyLanzhouChina
  2. 2.Lanzhou Institute of Husbandry and Pharmaceutical Science of CAASLanzhouChina

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