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Biological Trace Element Research

, Volume 189, Issue 1, pp 10–17 | Cite as

Determination of Trace Elements in Adipose Tissue of Obese People by Microwave-Assisted Digestion and Inductively Coupled Plasma Optical Emission Spectrometry

  • Agne Kizalaite
  • Vilma Brimiene
  • Gintautas Brimas
  • Jonas Kiuberis
  • Stasys Tautkus
  • Aleksej ZarkovEmail author
  • Aivaras Kareiva
Article
  • 310 Downloads

Abstract

In the present work, analytical method for the determination of trace elements in adipose tissue by means of inductively coupled plasma optical emission spectrometry (ICP-OES) was developed. Adipose tissue from two groups of the patients with obesity (with and without metabolic syndrome) was investigated. The main aim of this study was to reveal some differences and regularities in concentrations of trace elements in adipose tissue between these two groups of the individuals. Moreover, different types of adipose tissue (subcutaneous, preperitoneal, and visceral) were analyzed separately in order to investigate distribution of metals between these types in different groups of obese people. Al, Ba, Ca, Co, Cu, Cr, Fe, K, Li, Mg, Mn, Na, Ni, Sr, and Zn were selected for the quantitative determination. However, only 6 elements (Na, K, Ca, Fe, Mg, and Zn) were determined in all analyzed samples independently of the type of adipose tissue and presence of metabolic disorder of the patient. Concentrations of Ca, Fe, K, and Na were found to be strongly dependent on the type of adipose tissue. Other elements in terms of detection frequency in adipose tissue samples can be arranged in the following sequence Sr (94.7%), Cr (86.2%), Cu (24.6%), Li (18%), Ba (1.8%), Co (0.05%). The concentrations of Al, Mn, and Ni were found to be lower than limit of detection (LOD) in all analyzed samples. Comparison of metal distribution depending on the type of adipose tissue of people with and without metabolic syndrome is discussed in this work.

Keywords

Adipose tissue Obesity Metabolic syndrome Elemental analysis ICP-OES 

Notes

Funding Information

This work was supported by a grant SEMAT (No. SEN-02/2016) of National Research Programme “Healthy ageing” from the Research Council of Lithuania.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Agne Kizalaite
    • 1
  • Vilma Brimiene
    • 2
  • Gintautas Brimas
    • 3
  • Jonas Kiuberis
    • 1
  • Stasys Tautkus
    • 1
  • Aleksej Zarkov
    • 1
    Email author
  • Aivaras Kareiva
    • 1
  1. 1.Institute of ChemistryVilnius UniversityVilniusLithuania
  2. 2.Clinic of Gastroenterology, Nephrourology and Surgery, Faculty of Medicine, Center of Abdominal SurgeryVilnius UniversityVilniusLithuania
  3. 3.Clinic of Gastroenterology, Nephrourology and Surgery, Department of General SurgeryVilnius UniversityVilniusLithuania

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