Biological Trace Element Research

, Volume 187, Issue 2, pp 357–366 | Cite as

Investigations of Human Fascia Lata Elemental Composition—the Effect of Different Preservation and Mineralisation Methods

  • Anna LeśniewiczEmail author
  • Milena Furtak
  • Wiesław Żyrnicki
  • Joanna Dawidowicz
  • Krzysztof Maksymowicz
  • Sylwia Szotek


Influence of fixation medium and storage conditions as well as impact of sample mineralisation procedure on determination of minerals in human fascia lata was examined and discussed. Freezing and storage in 10% neutral buffered formalin solution and in 2.5% glutaraldehyde were used as the preservation methods of the samples. The concentrations of, both essential and toxic, elements were measured by ICP-OES method in fascia lata samples mineralised with concentrated nitric acid in a closed microwave system and in open vessels heated on a hot plate. Freezing was found as the best preserving method of fascia lata samples because of the number of elements determined and determination precision. The trace element (Cd, Cr, Cu, Fe, Ni, Sr, Zn) concentrations obtained in samples decomposed using the conventional hot plate were different from analogous measurements in solutions obtained after application of closed vessels and microwave energy assistance. Differences between the mineral compositions of fascia samples variously preserved and mineralised were statistically evaluated and discussed. Interelement correlations were analysed taking into account an impact of various methods of sample conservation. Strong, positive association between element content was discovered for Cr-Ba, Mn-Ba, P-Ba, Sr-Ba, Sr-Ca, Zn-Ca, Mn-Cr, Pb-Cr, Sr-Cr, Mg-Fe, P-Fe, Pb-Ni, Ti-Ni and Sr-P pairs of elements.


Human fascia lata Preservation and storage methods Mineralisation Elemental composition ICP-OES Interelelment correlations 



The work was financed by a statutory activity subsidy from the Polish Ministry of Science and Higher Education for the Faculty of Chemistry of Wrocław University of Science and Technology and by National Science Centre grant no. N N518 286540.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This study was approved by the Bioethics Committee of Wroclaw Medical University (KB-262/2010).

Statement of Human Rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Bioethics Committee of Wroclaw Medical University and with the 1964 Helsinki declaration and its later amendments.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Analytical Chemistry and Chemical Metallurgy Division, Faculty of ChemistryWrocław University of Science and TechnologyWrocławPoland
  2. 2.Veterinary Clinic “Brynów”KatowicePoland
  3. 3.Faculty of Medicine, Department of Forensic MedicineWrocław Medical UniversityWrocławPoland
  4. 4.Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Faculty of Mechanical EngineeringWrocław University of Science and TechnologyWrocławPoland

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