Biological Trace Element Research

, Volume 187, Issue 1, pp 74–79 | Cite as

Association Between Selenium and Malondialdehyde as an Efficient Biomarker of Oxidative Stress in Infantile Cardiac Surgery

  • Marlice Oliveira de Oliveira Ulbrecht
  • Daniel Araujo GonçalvesEmail author
  • Lourdes Zélia Garcia Zanoni
  • Valter Aragão do Nascimento


The present work describes a method to quantify the level of oxidative stress in infantile cardiac surgery. Fifteen patients, 6 girls and 9 boys, aged between 3 months and 16 years were divided into three groups. The first group sought to quantify the oxidative stress from differing concentrations of selenium. The second group used malondialdehyde as an indicator of oxidative stress. Finally, the third group quantified oxidative stress by normalizing the selenium concentration via malondialdehyde. Blood aliquots of 1.50 ml, drawn from the radial artery, were collected and centrifuged for quantification of Se and MDA in plasma. The statistical method ANOVA was used with a 95% confidence interval to indicate significant statistical differences between the post- and pre-operative stage for each group. The concentrations of malondialdehyde were measured by using UV-Vis following the thiobarbituric acid reaction method. For quantification of selenium, the samples were submitted to assisted microwave digestion and measured by ICP OES. In the first two groups, it was not possible to affirm that selenium and malondialdehyde could be biomarkers of oxidative stress, so a statistic test (ANOVA) was performed. However, the selenium/malondialdehyde ratios in the pre-operative and post-operative stage were 2.10 ± 0.70 and 3.20 ± 0.40, respectively. The ANOVA test confirmed a statistically significant difference between the pre- and post-operative stages with p value = 0.004. Here, the ratio of selenium concentration by malondialdehyde was confirmed to be an effective parameter for demonstration and quantification of oxidative stress activity at the post-operative stage.


Lipid peroxidation Infant cardiac surgery Blood ICP OES Selenium Malondialdehyde 



The authors would like to thank the School of Medicine, Federal University of Mato Grosso do Sul, for their support to this work. The fellowship provided to D.A.G. by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil, Grant No 1663746 and 1744738) is also greatly appreciated.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Sies H (2000) What is oxidative stress? In: Keaney JF (ed) Oxidative stress and vascular disease, Developments in cardiovascular medicine, vol vol 224. Springer, Boston, MACrossRefGoogle Scholar
  2. 2.
    Poprac P, Jomova K, Simunkova M, Kollar V, Rhodes CJ, Valko M (2017) Targeting free radicals in oxidative stress-related human diseases. Trends Pharmacol Sci 38(7):592–607CrossRefGoogle Scholar
  3. 3.
    Mihara M, Uchiyama M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86(1):271–278CrossRefGoogle Scholar
  4. 4.
    Del Rio D, Stewart AJ, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metb Cardiovas 15:316–328CrossRefGoogle Scholar
  5. 5.
    Tsikas D (2017) Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem 524(1):13–30CrossRefGoogle Scholar
  6. 6.
    Fedor M, Socha K, Urban B, Soroczy J, Matyskiela M, Borawska MH, Bakunowicz-Łazarczyk A (2017) Serum concentration of zinc, copper, selenium, manganese, and Cu/Zn ratio in children and adolescents with myopia. Biol Trace Elem Res 176(1):1–9CrossRefGoogle Scholar
  7. 7.
    Vijayasarathy K, Shanthi Naidu K, Sastry BK (2010) Melatonin metabolite 6-Sulfatoxymelatonin, Cu/Zn superoxide dismutase, oxidized LDL and malondialdehyde in unstable angina. Int J Cardiol 144(2):315–317CrossRefGoogle Scholar
  8. 8.
    Zhang SS, Noordin MM, Rahman SO, Haron J (2000) Effects of copper overload on hepatic lipid peroxidation and antioxidant defense in rats. Vet Hum Toxicol 42(5):261–264PubMedPubMedCentralGoogle Scholar
  9. 9.
    Meguid NA, Dardir AA, Abdel-Raouf ER, Hashish A (2011) Evaluation of oxidative stress in autism: defective antioxidant enzymes and increased lipid peroxidation. Biol Trace Elem Res 143(1):58–65CrossRefGoogle Scholar
  10. 10.
    Huang YL, Sheu JY, Lin TH (1999) Association between oxidative stress and changes of trace elements in patients with breast cancer. Clin Biochem 32(2):131–136CrossRefGoogle Scholar
  11. 11.
    Pocernich CB, Butterfield DA (2012) Elevation of glutathione as a therapeutic strategy in Alzheimer disease. Biochim Biophys Acta Mol basis Dis 1822(5):625–630CrossRefGoogle Scholar
  12. 12.
    Ozdemır G, Ozden M, Maral H, Kuskay S, Cetınalp P, Tarkun I (2005) Malondialdehyde, glutathione, glutathione peroxidase and homocysteine levels in type 2 diabetic patients with and without microalbuminuria. Ann Clin Biochem 42:99–104CrossRefGoogle Scholar
  13. 13.
    Machát J, Kanický V, Otruba V (2002) Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization. Anal Bioanal Chem 372:576–581CrossRefGoogle Scholar
  14. 14.
    Long GL, Winefordner JD (1983) Limit of detection a closer look at the IUPAC definition. Anal Chem 55(7):712–724Google Scholar
  15. 15.
    Safaralizadeh R, Kardar GA, Pourpak Z, Moin M, Zare A, Teimourian S (2005) Serum concentration of selenium in healthy individuals living in Tehran. Nutr J 32, 4(1)Google Scholar
  16. 16.
    Zanoni LZ, Melnikov P, Finotti TH, Cônsolo FZ, Silva AF, Nascimento VA (2016) Selenium in healthy individuals in Mato Grosso do Sul State, Brazil. European J Nutr Food Saf 6(1):36–40CrossRefGoogle Scholar
  17. 17.
    Brenneisen P, Steinbrenner H, Sies H (2005) Selenium, oxidative stress, and health aspects. Mol Asp Med 26:256–267CrossRefGoogle Scholar
  18. 18.
    Huang Z, Rose AH, Hoffmann PR (2012) The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 16(7):705–743CrossRefGoogle Scholar
  19. 19.
    Montes-Nieto R, Insenser M, Murri M, Fernández-Durán E, Ojeda-Ojeda M, Martínez-García MA, Luque-Ramírez M, Escobar-Morreale HF (2017) Plasma thiobarbituric acid reactive substances (TBARS) in young adults: obesity increases fasting levels only in men whereas glucose ingestion, and not protein or lipid intake, increases postprandial concentrations regardless of sex and obesity. Mol Nutr Food Res 61(11):1700425CrossRefGoogle Scholar
  20. 20.
    Uldebrecht MOO, Zanoni LZ, Gonçalves DA, Campos VP, Melo ESP, Guimarães RCA, Nascimento VA (2018) The importance of the study of plasma concentration of selenium and oxidative stress in cardiac surgery with children cardiopulmonary bypass. Int J Dev Res 8(1):18321–18325Google Scholar
  21. 21.
    Callejón-Leblic B, Rodríguez-Moro G, García-Barrera T, Gómez-Ariza JL (2018). Simultaneous speciation of selenoproteins and selenometabolites in plasma and serum. In: Chavatte L. (eds) Selenoproteins. Methods in molecular biology, Humana Press, New York, NY 661:163–175Google Scholar
  22. 22.
    Oldham KM, Eebowen P (1998) Oxidative stress in critical care: is antioxidant supplementation beneficial? J Am Diet Assoc 98(9):1001–1008CrossRefGoogle Scholar
  23. 23.
    McDonald CI, Fraser JF, Jeff S (2014). Coombes and Yoke Lin Fung. Oxidative stress during extracorporeal circulation. Eur J Cardiothorac Sur 46:937–943Google Scholar
  24. 24.
    Binder CJ (1984) Lipid modification and lipid peroxidation products in innate immunity and inflammation. Biochim Biophys Acta, Mol Cell Biol Lipids 4:369–370Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marlice Oliveira de Oliveira Ulbrecht
    • 1
  • Daniel Araujo Gonçalves
    • 1
    • 2
    Email author
  • Lourdes Zélia Garcia Zanoni
    • 1
  • Valter Aragão do Nascimento
    • 1
  1. 1.Group of Spectroscopy and Bioinformatics Applied Biodiversity and Health, School of MedicineFederal University of Mato Grosso do Sul—FAMED/UFMSCampo GrandeBrazil
  2. 2.Department of ChemistryMinas Gerais State University—UEMGItuiutabaBrazil

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