Biological Trace Element Research

, Volume 187, Issue 1, pp 120–127 | Cite as

Protective Effect of Rosa damascena Against Aluminum Chloride-Induced Oxidative Stress

  • Zeinab Zahedi-Amiri
  • Ali TaravatiEmail author
  • Leila Beigom Hejazian


Aluminum is considered an essential element endowed with toxicity potentials in human and animal. Thus, intoxication with aluminum can lead to oxidative stress, which is associated with oxidative damage to various macromolecules. Moreover, antioxidants from natural sources can play an important role in human health. Accordingly, the purpose of this study was to investigate the protective effect of Rosa damascena extract against aluminum-induced oxidative stress. In this study, 60 male rats were randomly divided into six groups and then they were given daily aluminum chloride and Rosa damascena extract. After 8 weeks of treatment, the levels of total antioxidant and malondialdehyde, as well as antioxidant enzymes including catalase, glutathione S-transferase, and myeloperoxidase, were measured in all experimental groups in this study. A significant increase was found in the total antioxidant level in the rats treated with aluminum, Rosa damascena extract, and aluminum plus Rosa damascena extract compared with those in the control group. Also, malondialdehyde levels were not significantly different in all the studied groups. Glutathione S-transferase activity levels in rats receiving the Rosa damascena extract as well as rats taking aluminum with Rosa damascena extract increased significantly compared with the ones in the control group. Catalase activity in the aluminum-treated group also increased significantly compared with the rates in the control group (31.34 ± 4.50 U/gHb vs. 14.04 ± 6.17 U/gHb, p = 0.014). Furthermore, myeloperoxidase activity in the aluminum-treated group increased significantly compared with the control group (49.47 ± 5.12 U/L vs. 25.28 ± 2.18 U/L, p < 0.001). The Rosa damascena extract could improve antioxidant capacity and reduce oxidative conditions in rats receiving aluminum chloride as evidenced by assays of the ferric reducing ability of plasma and activity of antioxidant enzymes. According to the findings of this study, it can be concluded that the Rosa damascena extract with its high antioxidant content is able to exert a protective effect against aluminum-induced oxidative stress.


Aluminum chloride Rosa damascene Antioxidants Oxidative stress 


  1. 1.
    Abdel-Wahab WM (2012) AlCl3-induced toxicity and oxidative stress in liver of male rats: protection by melatonin. Life Sci J 9(4):1173–1182Google Scholar
  2. 2.
    Xu F, Liu Y, Zhao H, Yu K, Song M, Zhu Y, Li Y (2017) Aluminum chloride caused liver dysfunction and mitochondrial energy metabolism disorder in rat. J Inorg Biochem 174:55–62CrossRefGoogle Scholar
  3. 3.
    Bondy SC, Campbell A (2017) Chapter five—aluminum and neurodegenerative diseases. In: Aschner M, Costa LG (eds) Advances in Neurotoxicology, vol 1. Academic Press, Cambridge, pp 131–156Google Scholar
  4. 4.
    Kalaiselvi A, Suganthy OM, Govindassamy P, Vasantharaja D, Gowri B, Ramalingam V (2014) Influence of aluminium chloride on antioxidant system in the testis and epididymis of rats. Iranian J Toxicol 8(24):991–997Google Scholar
  5. 5.
    Samet JM, Wages PA (2018) Oxidative stress from environmental exposures. Curr Opin Toxicol 7:60–66CrossRefGoogle Scholar
  6. 6.
    Esrefoglu M (2012) Oxidative stress and benefits of antioxidant agents in acute and chronic hepatitis. Hepat Mon 12(3):160–167CrossRefGoogle Scholar
  7. 7.
    Jan AT, Azam M, Siddiqui K, Ali A, Choi I, Haq QM (2015) Heavy metals and human health: mechanistic insight into toxicity and counter defense system of antioxidants. Int J Mol Sci 16(12):29592–29630CrossRefGoogle Scholar
  8. 8.
    Dehghan Kashani A, Rasooli I, Rezaee MB, Owlia P (2011) Antioxidative properties and toxicity of white rose extract. Iranian J Toxicol 5(12):415–425Google Scholar
  9. 9.
    Koksall N, Aslancan H, Sadighazadi S, Kafkas E (2015) Chemical investigation on Rose damascena Mill. volatiles; effects of storage and drying conditions. Acta Sci Pol Hortorum Cultus 14(1):105–114Google Scholar
  10. 10.
    Boskabady MH, Shafei MN, Saberi Z, Amini S (2011) Pharmacological effects of Rosa damascena. Iranian J Basic Med Sci 14(4):295–307Google Scholar
  11. 11.
    Memariani Z, Amin G, Moghaddam G, Hajimahmoodi M (2015) Comparative analysis of phenolic compounds in two samples of Rosa damascena by HPLC. Int J Biosci (IJB) 7(1):112–118CrossRefGoogle Scholar
  12. 12.
    Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76CrossRefGoogle Scholar
  13. 13.
    Buege JA, Aust SD (1978) [30] Microsomal lipid peroxidation. Methods Enzymol 52:302–310CrossRefGoogle Scholar
  14. 14.
    Aebi H (1984) [13] Catalase in vitro. Methods Enzymol 105:121–126CrossRefGoogle Scholar
  15. 15.
    Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases the first enzymatic step in mercapturic acid formation. J Biol Chem 249(22):7130–7139PubMedPubMedCentralGoogle Scholar
  16. 16.
    Bradley PP, Priebat DA, Christensen RD, Rothstein G (1982) Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Investig Dermatol 78(3):206–209CrossRefGoogle Scholar
  17. 17.
    Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4(8):118–126CrossRefGoogle Scholar
  18. 18.
    Zaidi SKR, Banu N (2004) Antioxidant potential of vitamins A, E and C in modulating oxidative stress in rat brain. Clin Chim Acta 340(1):229–233CrossRefGoogle Scholar
  19. 19.
    Kähkönen MP, Hopia AI, Vuorela HJ, Rauha J-P, Pihlaja K, Kujala TS, Heinonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47(10):3954–3962CrossRefGoogle Scholar
  20. 20.
    Flora S, Mittal M, Mehta A (2008) Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian J Med Res 128(4):501PubMedPubMedCentralGoogle Scholar
  21. 21.
    Exley C (2004) The pro-oxidant activity of aluminum. Free Radic Biol Med 36(3):380–387CrossRefGoogle Scholar
  22. 22.
    Mahmoud ME, Elsoadaa SS (2013) Protective effect of ascorbic acid, biopropolis and royal jelly against aluminum toxicity in rats. J Nat Sci Res 3(1):102–112Google Scholar
  23. 23.
    Guo-Ross S, Yang E, Bondy SC (1998) Elevation of cerebral proteases after systemic administration of aluminum. Neurochem Int 33(3):277–282CrossRefGoogle Scholar
  24. 24.
    Yuan C-Y, Hsu G-SW, Lee Y-J (2012) Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. J Biomed Sci 19(1):51CrossRefGoogle Scholar
  25. 25.
    Gutteridge JM, Quinlan GJ, Clark I, Halliwell B (1985) Aluminium salts accelerate peroxidation of membrane lipids stimulated by iron salts. Biochim Biophys Acta (BBA)-Lipids Lipid Metab 835(3):441–447CrossRefGoogle Scholar
  26. 26.
    Ali BH, Al-Salam S, Al Suleimani Y, Al Kalbani J, Al Bahlani S, Ashique M, Manoj P, Al Zhili B, Al Abri N, Naser HT (2018) Curcumin ameliorates kidney function and oxidative stress in experimental chronic kidney disease. Basic Clin Pharmacol Toxicol 122(1):65–73CrossRefGoogle Scholar
  27. 27.
    Maryam Sharafi S, Rasooli I, Dehghan Kashani A, Owlia P, Rezaee MB, Darvish Alipoor Astaneh S (2010) Cytobiochemical potentials of Rosa damascena Mill. extract. Iran J Pathol 5(4):184–193Google Scholar
  28. 28.
    Yang Y, Cheng J-Z, Singhal SS, Saini M, Pandya U, Awasthi S, Awasthi YC (2001) Role of glutathione S-transferases in protection against lipid peroxidation overexpression of HGSTA2-2 in K562 cells protects against hydrogen peroxide-induced apoptosis and inhibits JNK and caspase 3 activation. J Biol Chem 276(22):19220–19230CrossRefGoogle Scholar
  29. 29.
    Primiano T, Sutter TR, Kensler TW (1997) Antioxidant-inducible genes. Adv Pharmacol (San Diego, Calif) 38:293–328CrossRefGoogle Scholar
  30. 30.
    Palmer HJ, Paulson KE (1997) Reactive oxygen species and antioxidants in signal transduction and gene expression. Nutr Rev 55(10):353–361CrossRefGoogle Scholar
  31. 31.
    Nile SH, Keum YS, Nile AS, Jalde SS, Patel RV (2018) Antioxidant, anti-inflammatory, and enzyme inhibitory activity of natural plant flavonoids and their synthesized derivatives. J Biochem Mol Toxicol 32(1):e22002CrossRefGoogle Scholar
  32. 32.
    Pedraza-Chaverrí J, de los Ángeles Granados-Silvestre M, Medina-Campos ON, Maldonado PD, Olivares-Corichi IM, Ibarra-Rubio ME (2001) Post-transcriptional control of catalase expression in garlic-treated rats. Mol Cell Biochem 216(1):9–19CrossRefGoogle Scholar
  33. 33.
    Tang Q, Ji F, Wang J, Guo L, Li Y, Bao Y (2017) Quercetin exerts synergetic anti-cancer activity with 10-hydroxy camptothecin. Eur J Pharm Sci 109:223–232CrossRefGoogle Scholar
  34. 34.
    Lesjak M, Beara I, Simin N, Pintać D, Majkić T, Bekvalac K, Orčić D, Mimica-Dukić N (2018) Antioxidant and anti-inflammatory activities of quercetin and its derivatives. J Funct Foods 40:68–75CrossRefGoogle Scholar
  35. 35.
    Gao W, Pu L, Chen M, Wei J, Xin Z, Wang Y, Yao Z, Shi T, Guo C (2018) Glutathione homeostasis is significantly altered by quercetin via the Keap1/Nrf2 and MAPK signaling pathways in rats. J Clin Biochem Nutr 62(1):56–62CrossRefGoogle Scholar
  36. 36.
    Halder S, Kar R, Mehta AK, Bhattacharya SK, Mediratta PK, Banerjee BD (2016) Quercetin modulates the effects of chromium exposure on learning, memory and antioxidant enzyme activity in F1 generation mice. Biol Trace Elem Res 171(2):391–398CrossRefGoogle Scholar
  37. 37.
    Kumar A, Sehgal N, Kumar P, Padi S, Naidu P (2008) Protective effect of quercetin against ICV colchicine-induced cognitive dysfunctions and oxidative damage in rats. Phytother Res 22(12):1563–1569CrossRefGoogle Scholar
  38. 38.
    Mohammadi HS, Goudarzi I, Lashkarbolouki T, Abrari K, Salmani ME (2014) Chronic administration of quercetin prevent spatial learning and memory deficits provoked by chronic stress in rats. Behav Brain Res 270:196–205CrossRefGoogle Scholar
  39. 39.
    Surapaneni K, Jainu M (2014) Comparative effect of pioglitazone, quercetin and hydroxy citric acid on the status of lipid peroxidation and antioxidants in experimental non-alcoholic steatohepatitis. J Physiol Pharmacol 65(1):67–74PubMedPubMedCentralGoogle Scholar
  40. 40.
    Lai C-C, Peng M, Huang L, Huang W-H, Chiu TH (1996) Chronic exposure of neonatal cardiac myocytes to hydrogen peroxide enhances the expression of catalase. J Mol Cell Cardiol 28(5):1157–1163CrossRefGoogle Scholar
  41. 41.
    Aratani Y (2018) Myeloperoxidase: its role for host defense, inflammation, and neutrophil function. Arch Biochem Biophys 640:47–52CrossRefGoogle Scholar
  42. 42.
    Naskalski JW, Marcinkiewicz J, Drożdż R (2002) Myeloperoxidase-mediated protein oxidation: its possible biological functions. Clin Chem Lab Med 40(5):463–468CrossRefGoogle Scholar
  43. 43.
    Slavov A, Kiyohara H, Yamada H (2013) Immunomodulating pectic polysaccharides from waste rose petals of Rosa damascena Mill. Int J Biol Macromol 59:192–200CrossRefGoogle Scholar
  44. 44.
    Latifi G, Ghannadi A, Minaiyan M (2015) Anti-inflammatory effect of volatile oil and hydroalcoholic extract of Rosa damascena Mill. on acetic acid-induced colitis in rats. Res Pharm Sci 10(6):514–522PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Molecular and Cell Biology, Faculty of Basic SciencesUniversity of MazandaranBabolsarIran
  2. 2.Department of Anatomy, School of MedicineBabol University of Medical SciencesBabolIran

Personalised recommendations