Biological Trace Element Research

, Volume 186, Issue 1, pp 258–266 | Cite as

Effects of Supplemental Epigallocatechin Gallate in the Diet of Broilers Exposed to Fluoride Intoxication

  • İlkay AydoganEmail author
  • Mehmet Akif Karslı
  • Mehmet Başalan
  • Ebru Yıldırım
  • Miyase Çınar
  • Gökhan Şen
  • Tugce Sümer


We evaluated the effects of dietary epigallocatechin gallate (EGCG) on the performance, biochemical parameters, and liver histopathology of fluoride-intoxicated broiler chickens. In total, 160 1-day-old male broiler chicks (Ross PM3 strain) were collected and assigned to four groups (40 animals each), with four replicates. The control group received a basal diet; the F group received 800 mg/kg fluoride; the EGCG group received 400 mg/kg EGCG; and the EGCG+F group received 400 mg/kg EGCG and 800 mg/kg fluoride. The live weight (LW) of F-treated chicks was significantly lower than that of the controls. In the F-treated groups, feed intake (FI) and LW values were lower, but the feed conversion ratio (FCR) was higher than those of the controls. The ratio of heart weight to LW was found to be the highest in the F-treated groups. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), and total oxidant status (TOS) levels in the F-treated groups were significantly higher, whereas the increase in total cholesterol levels was insignificant than those in the control group. In the EGCG+F group, AST, total cholesterol, and TOS levels decreased to a level comparable to those in the control group. Histopathological evaluation revealed that there were mild changes in the portal region in the EGCG+F group; additionally, there was an improvement in liver morphology in the EGCG+F group compared to that in the F group. Thus, EGCG has potent antioxidant and regenerative effects that can ameliorate the detrimental effects of fluoride toxicity on blood parameters and the liver.


Antioxidant status Biochemical parameters Broiler performance Epigallocatechin Fluoride toxicity Liver histopathology 


Funding Information

This research was supported by the Scientific Research Fund in the University of Kirikkale (Project No: 2014/126).

Compliance with Ethical Standards

The study protocol was approved by the Kırıkkale University Ethics Committee (Protocol Number: 2014/41).


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • İlkay Aydogan
    • 1
    Email author
  • Mehmet Akif Karslı
    • 1
  • Mehmet Başalan
    • 1
  • Ebru Yıldırım
    • 2
  • Miyase Çınar
    • 3
  • Gökhan Şen
    • 1
  • Tugce Sümer
    • 4
  1. 1.Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary MedicineKırıkkale UniversityKırıkkaleTurkey
  2. 2.Department of Pharmacology and Toxicology, Faculty of Veterinary MedicineKırıkkale UniversityKırıkkaleTurkey
  3. 3.Department of Biochemistry, Faculty of Veterinary MedicineKırıkkale UniversityKırıkkaleTurkey
  4. 4.Department of Pathology, Faculty of Veterinary MedicineKırıkkale UniversityKırıkkaleTurkey

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