Biological Trace Element Research

, Volume 185, Issue 1, pp 116–123 | Cite as

Protective Effects of Selenium on Cyclophosphamide-Induced Oxidative Stress and Kidney Injury

  • Sibel GunesEmail author
  • Varol Sahinturk
  • Sema Uslu
  • Adnan Ayhanci
  • Sedat Kacar
  • Ruhi Uyar


Cyclophosphamide (CP) is a common anticancer drug, but its use in cancer treatment is limited due to its severe toxicities induced mainly by oxidative stress in normal cells. Reactive oxygen species (ROS) lead to multiple organ injuries, including the kidneys. Selenium (Se) is a nutritionally essential trace element with antioxidant properties. In the present study, the possible protective effect of Se on CP-induced acute nephrotoxicity was investigated. Forty-two Sprague-Dawley rats were equally divided into six groups of seven rats in each. The control group received saline, and other groups were injected with CP (150 mg/kg), Se (0.5 or 1 mg/kg), or CP + Se intraperitoneally. Total antioxidant capacity (TAC), total oxidant state (TOS), oxidative stress index (OSI), creatinine, and cystatin C (Cys C) levels were measured in the sera. In addition, kidney tissues were examined histologically. In the CP alone treated rats, creatinine, Cys C, TOS, and OSI levels increased, while TAC level decreased. CP-induced histological damages were decreased by co-treatment of Se and biochemical results supported the microscopic observations. In conclusion, our study points to the therapeutic potential of Se and indicates a significant role of ROS in CP-induced kidney toxicity.


Cyclophosphamide Oxidative stress Nephrotoxicity Selenium Creatinine Cystatin C 



This work was supported by Eskisehir Osmangazi University Scientific Research Project Commission, Eskisehir, Turkey, with the project number 2014-254.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Faculty of Arts and Science Department of BiologyEskisehir Osmangazi UniversityEskisehirTurkey
  2. 2.Faculty of Medicine Department of Histology and EmbryologyEskisehir Osmangazi UniversityEskisehirTurkey
  3. 3.Faculty of Medicine Department of BiochemistryEskisehir Osmangazi UniversityEskisehirTurkey
  4. 4.Faculty of Medicine Department of PhysiologyEskisehir Osmangazi UniversityEskisehirTurkey

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