Biological Trace Element Research

, Volume 185, Issue 1, pp 226–235 | Cite as

Determination of Heavy Metals in Almonds and Mistletoe as a Parasite Growing on the Almond Tree Using ICP-OES or ICP-MS

  • Veysi Kamar
  • Rukiye Dağalp
  • Mustafa TaştekinEmail author


In this study, the elements of Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Ni, Sr, Pb, Ti, and Zn were determined in the leaves, fruits, and branches of mistletoe, (Viscum albüm L.), used as a medicinal plant, and in the leaves, branches and barks of almond tree which mistletoe grows on. The aim of the study is to investigate whether the mistletoe are more absorbent than the almond tree in terms of the heavy metal contents and the determination of the amount of the elements penetrated into the mistletoe from the almond tree. ICP-MS (inductively coupled plasma-mass spectrometry) was used for the analysis of As, Cd, Mo, and Pb, whereas ICP-OES (inductively coupled plasma optical emission spectrometry) was used for the other elements. The results obtained were statistically evaluated at 95% confidence level. Within the results obtained in this study, it was determined whether there is a significant difference between metal elements in almond tree and mistletoe, or not. As a result, it was observed that there were higher contents of B, Ba, K, Mg, and Zn in the mistletoe than in the almond tree. K was found much higher than other elements in the mistletoe. On the other hand, Al, As, Ca, Cd, Cr, Cu, Fe, Mo, Ni, Sr, Pb, and Ti contents were determined to be more in almond tree than mistletoe.


Almond tree Mistletoe Heavy metals Determination ICP-OES and ICP-MS 


Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Obbard JB (2001) Ecotoxicological assessment of heavy metals in sewage sludge amended soils. Appl Geochem 16(11-12):1405–1411,58. CrossRefGoogle Scholar
  2. 2.
    Senesi GS, Baldassarre G, Senesi N, Radina B (1999) Trace element inputs into soils by anthropogenic activities and implications for human health. Chemosphere 39(2):343–337. CrossRefPubMedGoogle Scholar
  3. 3.
    Anonymous. 2002. European Commission DG ENV, E3 Project ENV.E.,3/ETU/2000/0058, “Heavy Metals in Waste” February, DenmarkGoogle Scholar
  4. 4.
    Gamalero, E., Lingua, G., Berta, G., Glick, B. R., 2009. Beneficial role of plant growth promoting bacteria and arbuscular mycorrhizal fungi on plant responses to heavy metal stress, Canadian Journal of Microbiology, 55(5) 501–514Google Scholar
  5. 5.
    Arain MB, Kazi TG (2008) Time saving modified BCR sequential extraction procedure for the fraction of Cd, Cr, Cu, Ni, Pb and Zn in sediment samples of polluted lake. J Hazard Mater 160(1):235–239. CrossRefPubMedGoogle Scholar
  6. 6.
    Yu L, Yan-bin W, Xin G, Yi-bing S, Gang W (2006) Risk assessment of heavy metals in soils and vegetables around non-ferrous metals mining and smelting sites, Baiyin, China. J Environ Sci 18:1124–1134CrossRefGoogle Scholar
  7. 7.
    Goldman P (2001) Herbal medicines today and the roots of modern pharmacology. Ann Intern Med 135(8_Part_1):594–600. CrossRefPubMedGoogle Scholar
  8. 8.
    Gomez RM, Cerutti S, Sombra LL, Silva M, Martinez LD (2007) Determination of heavy metals for the quality control in Argentinian herbal medicines by ETAAS and ICP-OES. Food Chem Toxicol 45(6):1060–1064. CrossRefPubMedGoogle Scholar
  9. 9.
    Ergun, F., Deliorman, D., Şener, B. 1994. Some studies on morphological characters of Viscum albüm L, (Loranthaceae) and its distribution in Turkey, The Herb Journal of Syst Bot, 1 (2): 47–62Google Scholar
  10. 10.
    Jamison J. R. 2003. Clinical guide to nutrition and dietary supplements in disease management. ISSN: 0443071934,Churchill Livingstone; 1 editionGoogle Scholar
  11. 11.
    Miller AG (1982) Loranthaceae. In: Davis PH (ed) Flora of Turkey and the East Aegean Islands, 7th edn. University Press, Edinburgh, pp 546–549Google Scholar
  12. 12.
    Mandacı, S. 1998. Balıkesir İÜ Tarım ve Orman Alanlarında Ökseotları, Zararları, Koruma ve Savaş Yöntemleri, "Yüksek Lisans Tezi" Uludağ Üniversitesi Fen Bilimleri Enstitüsü, BursaGoogle Scholar
  13. 13.
    Bowen ME, McAlpine CA, House APN, Smith GC (2009) Agricultural landscape modification increases the abundance of an important food resource: mistletoes, birds and brigalow. Biol Conserv 142(1):122–133. CrossRefGoogle Scholar
  14. 14.
    Leea JS, Chona HT, Kimb KW (1998) Migration and dispersion of trace elements in the rock–soil–plant system in areas underlain by black shales and slates of the Okchon Zone, Korea. J Geochem Explor 65(1):61–78. CrossRefGoogle Scholar
  15. 15.
    García D, Rodríguez‐Cabal MA, Amico GC (2009). Seed dispersal by a frugivorous marsupial shapes the spatial scale of a mistletoe population, Journal of Ecology, 97(2) 217–229Google Scholar
  16. 16.
    Khwaja TA, Varven JC, Pentecost S, Pande H (1980) Isolation of biologically active Alkoloids from Korean mistletoe Viscum album, coloratum. Experientia 36(5):599–600. CrossRefPubMedGoogle Scholar
  17. 17.
    Hegi G (1981) Illustrierte Flora von Mitteleuropa III, 309–321. Parey, BerlinGoogle Scholar
  18. 18.
    Büssing A, Raak C, Ostermann T (2012) Quality of life and related dimensions in cancer patients treated with mistletoe extract (iscador): a meta-analysis. Evid Based Complement Alternat Med 2012:219402–219408. PubMedCrossRefGoogle Scholar
  19. 19.
    Eisenbraun J, Scheer R, Kröz M, Schad F, Huber R (2011) Quality of life in breast cancer patients during chemotherapy and concurrent therapy with a mistletoe extract. Phytomedicine 18(2–3):151–157. CrossRefPubMedGoogle Scholar
  20. 20.
    Melzer J, Iten F, Hostanska K, Saller R (2009) Efficacy and safety of mistletoe preparations (Viscum album) for patients with cancer diseases. A systematic review. Forsch Komplementmed 16(4):217–226. PubMedCrossRefGoogle Scholar
  21. 21.
    Ostermann, T. and Büssing, A., 2012. Retrolective studies on the survival of cancer patients treated with mistletoe extracts: a meta-analysis. Explore (NY), 8(5), 277–281, DOI:
  22. 22.
    Son, G. S., Ryu, W. S., Kim, H. Y., Woo, S. U., Park, K. H. and Bae, J. W. 2010. Immunologic response to mistletoe extract (Viscum album L.) after conventional treatment in patients with operable breast cancer, J. Breast Cancer, 13(1) 14–18, DOI:
  23. 23.
    Rote L (1994) Editio Cantor. Aulendorflwürtt 1994Google Scholar
  24. 24.
    Gorter RW, Van Wely M, Reif M (1999) Tolerability of an extract of European mistletoe among immunocompromised and healthy individuals. Altern Ther Health Med 5(6):37–44 47-8PubMedGoogle Scholar
  25. 25.
    Bar-Sela G, Wollner M, Hammer L, Agbarya A, Dudnik E, Haim N (2013) Mistletoe as complementary treatment in patients with advanced non-small-cell lung cancer treated with carboplatin-based combinations: a randomised phase II study. Eur J Cancer 49(5):1058–1064. CrossRefPubMedGoogle Scholar
  26. 26.
    Gunver SK, Helmut K (2010) Review article: influence of Viscum album L (European mistletoe) extracts on quality of life in cancer patients: a systematic review of controlled clinical studies. Integ Cancer Ther 9(2):142–157CrossRefGoogle Scholar
  27. 27.
    Marvibaigi, M., Supriyanto,E.,Amini, N., Majid, F. A. A. and Jaganathan, S.K., 2014. Preclinical and clinical effects of mistletoe against breast cancer, BioMed Research International,:785479, 15 pages, doi:
  28. 28.
    Temür N (2006) Çam, kavak, söğüt ve armut ağaçları üzerinde yetişen ökse otu (Viscum album L,) bitkilerinin antioksidan aktivitelerinin incelenmesi. Gaziosmanpaşa Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, TokatGoogle Scholar
  29. 29.
    Jurin M, Zarkovic N, Hrzenjak M, Blic Z (1993) Antitumorous and immunomodulatory effects of the Viscum album L, preparation Isorel. Oncology 50(6):393–398. CrossRefPubMedGoogle Scholar
  30. 30.
    Bar-Sela G, Gershony A, Haim N (2006) Mistletoe (Viscum album) preparations: an optional drug for cancer patients? Harefuah 145(1):42–46 77. Review. HebrewPubMedGoogle Scholar
  31. 31.
    Semiglazov VF, Stepula VV, Dudov A, Schnitker J, Mengs U (2006) Quality of life is improved in breast cancer patients by Standardised Mistletoe Extract PS76A2 during chemotherapy and follow-up: a randomised, placebo-controlled, double-blind, multicentre clinical trial. Anticancer Res 26(2 B):1519–1529PubMedGoogle Scholar
  32. 32.
    Vester F, El-Fouly Bohnel M (1968) Zurkenntris der inhalt Staffe von Viscum album, IV Biologisches verhalten einzelner protein fraktionen, Hoppe-SeylerÕs, Z. Physiol Chem 349(1):495–511. CrossRefGoogle Scholar
  33. 33.
    Gray AM, Flatt PR (1999) Insulin-secreting activity of the traditional antidiabetic plant Viscum album (mistletoe). J Endocrinol 160(3):409–414. CrossRefPubMedGoogle Scholar
  34. 34.
    Büssing A, Schietzel M (1999) Apoptosis-inducing properties of Viscum album L. extracts from different host trees, correlate with their content of toxic mistletoe lectins. Anticancer Res 19(1A):23–28PubMedGoogle Scholar
  35. 35.
    Önay-Uçar E, Karagöz A, Arda N (2006) Antioxidant activity of viscum album ssp. album. Fitoterapia 77(7-8):556–560. CrossRefPubMedGoogle Scholar
  36. 36.
    Hamprecht K, Handgretinger R, Voetsch W, Anderer FA (1987) Mediation of human NK-activity by components in extracts of viscum album. Int J Immunopharmacol 9(2):199–209. CrossRefPubMedGoogle Scholar
  37. 37.
    Kovacs E, Hajto T, Hostanska K (1991) Improvement of DNA repair in lymphocytes of breast cancer patients treated with Viscum album extract (Iscador). Eur J Cancer 27(12):1672–1676CrossRefPubMedGoogle Scholar
  38. 38.
    Çelebioğlu, H. U. 2012. Effect of medicinal plants epilobium hirsutum and viscum album on rat liver microsomal flavin monooxygenase (fmo) activity and expression. Msc Thesisin Biology, Graduate School of Natural and Applied Sciences of Middle East Technical UniversityGoogle Scholar
  39. 39.
    Knöpfl-Sidler F, Viviani A, Rist L, Hensel A (2005) Human cancer cells exhibit in vitro individual receptiveness towards different mistletoe extracts. Pharmazie. Jun 60(6):448–454
  40. 40.
    Kienle GS, Glockmann A, Schink M, Kiene H (2009) Viscum album L. extracts in breast and gynaecological cancers: a systematic review of clinical and preclinical research. J Exp Clin Cancer Res 28:79. CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Kienle GS, Grugel R, Kiene H (2011) Safety of higher dosages of Viscum album L in animals and humans—systematic review of immune changes and safety parameters. BMC Complement Altern Med 11(1):72CrossRefPubMedPubMedCentralGoogle Scholar
  42. 42.
    Matthes H, Friedel WE, Bock PR, Zänker KS (2010) Molecular mistletoe therapy: friend or foe in established anti-tumor protocols? A multicenter, controlled, retrospective pharmacoepidemiological study in pancreas cancer. Curr Mol Med 10(4):430–439. CrossRefPubMedGoogle Scholar
  43. 43.
    Grossarth-Maticek R, Ziegler R (2007) Prospective controlled cohort studies on long-term therapy of ovairian cancer patients with mistletoe (Viscum album L.) extracts iscador. Arzneimittelforschung 57(10):665–678. PubMedCrossRefGoogle Scholar
  44. 44.
    Kunze E, Schulz H, Ahrens H, Gabıus H-J (1997) Lack of an antitumotral effect of immunmodulatory galactoside-spesific mistletoe lectin on N-methyl-N-nitrosourea-induced urinary bladder carcinogenesis in rats. Toxic Pathol 49(3-4):167–180. CrossRefGoogle Scholar
  45. 45.
    Molassiotis A, Scott JA, Kearney N, Pud D, Magri M, Selvekerova S, Bruyns I, Fernadez-Ortega P, Panteli V, Margulies A, Gudmundsdottir G, Milovics L, Ozden G, Platin N, Patiraki E (2006) Complementary and Alternative Medicine use in Breast Cancer Patients in Europe. Support Care Cancer 14(3):260–267CrossRefPubMedGoogle Scholar
  46. 46.
    Skoog D, Holler FJ, Nieman TA (1998) Principles of Instrumental, Çeviri Editörleri, Kılıç E, Köseoglu F, Yılmaz H. Bilim Yayıncılık, Ankara, 230-251Google Scholar
  47. 47.
    D’angelo JA, Martinez LD, Resnizky S, Perino E, Marchewsky EJ (2001) Determination of eight lanthanides in apatites by ICP-AES, XRF and NAA. J, Trace Microprobe Techn 19:79–90CrossRefGoogle Scholar
  48. 48.
    Lara, R.F, Wuilloud, R.G., Salonia, J.A., Olsina, R.A, ve Martinez, L.D. 2001. Determination of low cadmium concentrations in wine by on line preconcentration in a knotted reactor coupled to an inductively coupled plasma optical emission spectrometer with ultrasonic nebulization, Fresenius J,Analytical Chem., 371: 989–993Google Scholar
  49. 49.
    Montgomery DC (2004) Design and analysis of experiments, 6th edn. John Wiley & Sons Inc, NJ ISBN-10: 047148735XGoogle Scholar
  50. 50.
    Başgel S, Erdemoğlu SB (2006) Determination of mineral and trace elements in some medicinal herbs and their demlemes consumed in Turkey. Science of theTotal Environment 359(1-3):82–89. CrossRefGoogle Scholar
  51. 51.
    Malik, J., Frankova, A., Drabek, O., Szakova, J., Ash, C., Kokoska, L.2013. Aluminium and other elements in selected herbal tea plant species and their infusions Czech RepublicGoogle Scholar
  52. 52.
    Parthik P., Patel N.M., Patel P.M. 2011. WHO guidelines on quality control of herbal medicines, IJRAP, 2 (4) 1148–1154 Department of quality assurance BMCPER Modasa, IndiaGoogle Scholar
  53. 53.
    Tokalıoğlu, Ş. 2012. Determination of trace elements in commonly consumed medicinal herbs by ICP-MS and multivariate analysis, Food Chem 2012;134(4):2504–2508. doi:
  54. 54.
    Kabata-Pendias A, Mukherjee AB (2007) Trace element for soil to human, ISBN:3540327134. Springer, New York. CrossRefGoogle Scholar
  55. 55.
    Deveci T 2012Determination of trace elements (Cu, Co, Mn and Zn) and iron concentrations in soil and vegetables irrigated with domestic and industrial wastewaters of Gaziantep City, MSc. Thesis, Kilis 7 Aralık University Graduate School of Natural and Applied Sciences Department of Chemistry, TURKEYGoogle Scholar
  56. 56.
    Belitz HD, Grosch W, Schieberle P (2009) Food Chemıstry, 3rd edn. Springer, BerlinGoogle Scholar
  57. 57.
    Jabeen, S., Shah, M.T., Khan, S., Hayat M.K. 2010. Determination of major and trace elements in ten important folk therapeutic plants of Haripur basin, PakistanGoogle Scholar
  58. 58.
    Blicharska, E., Komsta, L., Kocjan, R., Gumieniczek, A., Robak, A. 2010. A preliminary study on the effect of mineralization parameters on determination of metals in Viscum Album species 20–081 Lublin-PolandGoogle Scholar
  59. 59.
    Garg AN, Kumar AGC, Reddy AVR (2007) Analysis of come Indian medicinal herbs by INAA. J Radioanal Nucl Chem 271(3):611–619. CrossRefGoogle Scholar
  60. 60.
    Fei, T., Dehong, L., Fengqun, Z., Junhua, L., Hua,T., Xiangzhong, K. 2010. Determination of trace elements in Chinesse medicinal plants by instrumental neutron activation analysıs. J Radioanal Nucl Chem, 284, 507–511, 3, DOI:
  61. 61.
    Street, R.A., Kulkarni, M.G., Stirk, W.A., Southway, C., Staden, J.V. 2008. Variation in heavy metals and microelements in South African medicinal plants obtained from street markets. Research Centre for plant growth and development, school of biological and conservation sciences, School of Chemistry University of Kwazulu-Natal pietemaritzburg, Scottsville 3201, South Africa, 25, 8, 953, 960, DOI:
  62. 62.
    Xie JH, Shen MY, Xie MY, Nie SP, Chen Y, Li C, Huang DF, Wang YX (2012) Ultrasonicassisted extraction, antimicrobial and antioxidant activity of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides. Carbohydr Polym 89(1):177–184. CrossRefPubMedGoogle Scholar
  63. 63.
    Walter, M., 2012. Trace elements in human and animal nutrition, Burlington Elsevier Science Volume 2Google Scholar
  64. 64.
    Chen T, Liu X, Zhu M, Zhao K, Wu J, Xu J, Huang P (2008) Identification of trace element sources and associated risk assessment in vegetable soils of the urban–rural transitional area of Hangzhou, China. Environ Pollut 151(1):67–78. CrossRefPubMedGoogle Scholar
  65. 65.
    Hamid, A., Riaz, H., Akhtar, S. and Ahmad, S. R 2016. Heavy metal contamination in vegetables, soil and water and potential health risk assessment, Am Eurasian J Agric and Environ Sci, 16 (4): 786–794Google Scholar
  66. 66.
    March WA, Watson DM (2010) The contribution of mistletoes to nutrient returns: evidence for a critical role in nutrient cycling. Ecol Soc Aust 35(7):713–721CrossRefGoogle Scholar
  67. 67.
    Vidal-Russell R, Nickrent DL (2008) The first mistletoes: origins of aerial parasitism in Santalales. Mol Phylogenet Evol 47(2):523–537. CrossRefPubMedGoogle Scholar
  68. 68.
    Bannister P, Strong GL (2001) Carbon and nitrogen isotope ratios, nitrogen content and heterotrophy in New Zealand mistletoes. Oecologia 126(1):10–20. CrossRefPubMedGoogle Scholar
  69. 69.
    Matsubara S, Morosinotto T, Bassi R, Christian AL, Fischer-Schliebs E, Lüttge U, Orthen B, Franco AC, Scarano FR, Förster B, Pogson BJ, Osmond CB (2003) Occurrence of the lutein-epoxide cycle in mistletoes of the Loranthaceae and Viscaceae. Planta 217(6):868–879. CrossRefPubMedGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Veysi Kamar
    • 1
  • Rukiye Dağalp
    • 2
  • Mustafa Taştekin
    • 1
    Email author
  1. 1.Department of Chemistry, Faculty of ScienceAnkara UniversityAnkaraTurkey
  2. 2.Department of Statistics, Faculty of ScienceAnkara UniversityAnkaraTurkey

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