Preparation and Optimization of a Biomimetic Triple-Layered Vascular Scaffold Based on Coaxial Electrospinning

  • Qingxi Hu
  • Chuang Wu
  • Haiguang ZhangEmail author


Electrospinning is a promising method for preparing bionic vascular scaffolds. In particular, coaxial electrospinning can encapsulate polymer materials in biological materials and provide vascular scaffolds with good biomechanical properties. However, it is difficult to produce a stable Taylor cone during the coaxial electrospinning process. Moreover, glutaraldehyde cross-linked natural biomaterials are cytotoxic. To address these issues, a novel electrospinning process is proposed in this report. A non-ionic surfactant (Tween 80) was added to poly(lactic-co-glycolic acid) electrospinning solution and gelatin-collagen electrospinning solution, which prevented the interfacial effect of coaxial electrospinning due to different core/shell solutions. The as-prepared materials were then cross-linked with the non-toxic coupling agents N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide/N-hydroxysuccinimide (EDC/NHS). By comparing the biomechanical properties of EDC/NHS cross-linked vascular scaffold with glutaraldehyde vapor–cross-linked vascular scaffold, it was found that the fracture strain and biological performance of EDC/NHS cross-linked vascular scaffold were better than those of the glutaraldehyde cross-linked scaffold. Finally, a three-layer bionic vascular scaffold was prepared by the proposed electrospinning process. Biomechanical performance tests were carried out and the prepared scaffold was found to meet the requirements of tissue-engineered blood vessels. The research in this paper provides a useful reference for the preparation and optimization of vascular scaffolds.


Tissue engineering Coaxial electrospinning EDC/NHS Vascular scaffold Tween 80 Endothelial cells 


Funding Information

This work was supported by the National Natural Science Foundation of China (grant no. 51775324).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


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Authors and Affiliations

  1. 1.Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and AutomationShanghai UniversityShanghaiChina
  2. 2.Shanghai Key Laboratory of Intelligent Manufacturing and RoboticsShanghai UniversityShanghaiChina
  3. 3.National Demonstration Center for Experimental Engineering Training EducationShanghai UniversityShanghaiChina

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